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• W2045554934 abstract "We have studied the diffusion and aggregation of H-2Kk antigens labeled with a fluorescent anti-H-2Kk monoclonal antibody (IgG) on mouse splenic lymphocytes, employing fluorescence photobleaching recovery and fluorescence microscopy. The H-2Kk antigens were initially distributed homogeneously on all lymphocytes. Upon antibody binding, sub-micron patches were formed on 50-60% of the cells. A lateral diffusion coefficient, D, of 7.1 X 10(-10) cm2/s and a mobile fraction of 0.73 were found for H-2Kk antigens on diffusely-labeled cells, while these antigens were immobile (D less than or equal to 5 X 10(-12) cm2/s) on patched cells. The patched and nonpatched sub-populations did not correspond to B- and T-lymphocytes. Subjection to low temperature or treatment with NaN3 or cytoskeleton-disrupting drugs did not affect the diffusion or patching of H-2Kk, indicating no involvement of metabolic energy or drug-sensitive cytoskeletal components. These findings could be related to the interactions of H-2 antigens on the cell surface, and to the different susceptibilities of various cells to lysis by cytotoxic T-cells." @default.
• W2045554934 created "2016-06-24" @default.
• W2045554934 creator A5004637588 @default.
• W2045554934 creator A5032368032 @default.
• W2045554934 date "1983-04-01" @default.
• W2045554934 modified "2023-09-26" @default.
• W2045554934 title "Lateral diffusion and patch formation of H-2Kk antigens on mouse spleen lymphocytes" @default.
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• W2045554934 doi "https://doi.org/10.1016/0167-4889(83)90082-4" @default.
• W2045554934 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6600938" @default.
• W2045554934 hasPublicationYear "1983" @default.
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