FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2045556022> ?p ?o ?g. }

• W2045556022 endingPage "817" @default.
• W2045556022 startingPage "807" @default.
• W2045556022 abstract "Channelrhodopsins serve as photoreceptors that control the motility behavior of green flagellate algae and act as light-gated ion channels when heterologously expressed in animal cells. Here, we report direct measurements of proton transfer from the retinylidene Schiff base in several channelrhodopsin variants expressed in HEK293 cells. A fast outward-directed current precedes the passive channel current that has the opposite direction at physiological holding potentials. This rapid charge movement occurs on the timescale of the M intermediate formation in microbial rhodopsins, including that for channelrhodopsin from Chlamydomonas augustae and its mutants, reported in this study. Mutant analysis showed that the glutamate residue corresponding to Asp(85) in bacteriorhodopsin acts as the primary acceptor of the Schiff-base proton in low-efficiency channelrhodopsins. Another photoactive-site residue corresponding to Asp(212) in bacteriorhodopsin serves as an alternative proton acceptor and plays a more important role in channel opening than the primary acceptor. In more efficient channelrhodopsins from Chlamydomonas reinhardtii, Mesostigma viride, and Platymonas (Tetraselmis) subcordiformis, the fast current was apparently absent. The inverse correlation of the outward proton transfer and channel activity is consistent with channel function evolving in channelrhodopsins at the expense of their capacity for active proton transport." @default.
• W2045556022 created "2016-06-24" @default.
• W2045556022 creator A5007213781 @default.
• W2045556022 creator A5019629089 @default.
• W2045556022 creator A5025433608 @default.
• W2045556022 creator A5034249208 @default.
• W2045556022 creator A5059177152 @default.
• W2045556022 date "2013-02-01" @default.
• W2045556022 modified "2023-10-16" @default.
• W2045556022 title "Intramolecular Proton Transfer in Channelrhodopsins" @default.
• W2045556022 cites W1487361437 @default.
• W2045556022 cites W1513594391 @default.
• W2045556022 cites W1965562809 @default.
• W2045556022 cites W1991421059 @default.
• W2045556022 cites W1997537106 @default.
• W2045556022 cites W1998794621 @default.
• W2045556022 cites W1999116701 @default.
• W2045556022 cites W1999758383 @default.
• W2045556022 cites W2011284621 @default.
• W2045556022 cites W2015714781 @default.
• W2045556022 cites W2021291965 @default.
• W2045556022 cites W2024465628 @default.
• W2045556022 cites W2030339127 @default.
• W2045556022 cites W2031757246 @default.
• W2045556022 cites W2039347400 @default.
• W2045556022 cites W2043358402 @default.
• W2045556022 cites W2051535344 @default.
• W2045556022 cites W2052423933 @default.
• W2045556022 cites W2053811816 @default.
• W2045556022 cites W2058488568 @default.
• W2045556022 cites W2060716559 @default.
• W2045556022 cites W2060842872 @default.
• W2045556022 cites W2060847850 @default.
• W2045556022 cites W2062594525 @default.
• W2045556022 cites W2064692246 @default.
• W2045556022 cites W2066076741 @default.
• W2045556022 cites W2068522285 @default.
• W2045556022 cites W2070341971 @default.
• W2045556022 cites W2071851347 @default.
• W2045556022 cites W2073650799 @default.
• W2045556022 cites W2074490675 @default.
• W2045556022 cites W2078944513 @default.
• W2045556022 cites W2085493691 @default.
• W2045556022 cites W2088944569 @default.
• W2045556022 cites W2091875427 @default.
• W2045556022 cites W2094076632 @default.
• W2045556022 cites W2096146737 @default.
• W2045556022 cites W2097583793 @default.
• W2045556022 cites W2104579888 @default.
• W2045556022 cites W2128377068 @default.
• W2045556022 cites W2130195611 @default.
• W2045556022 cites W2132622695 @default.
• W2045556022 cites W2148136351 @default.
• W2045556022 cites W2152762124 @default.
• W2045556022 cites W2155072940 @default.
• W2045556022 cites W2158550713 @default.
• W2045556022 cites W2159423248 @default.
• W2045556022 cites W2165273112 @default.
• W2045556022 cites W2167495642 @default.
• W2045556022 cites W2370991708 @default.
• W2045556022 cites W4318373887 @default.
• W2045556022 doi "https://doi.org/10.1016/j.bpj.2013.01.002" @default.
• W2045556022 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3576534" @default.
• W2045556022 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23442959" @default.
• W2045556022 hasPublicationYear "2013" @default.
• W2045556022 type Work @default.
• W2045556022 sameAs 2045556022 @default.
• W2045556022 citedByCount "59" @default.
• W2045556022 countsByYear W20455560222013 @default.
• W2045556022 countsByYear W20455560222014 @default.
• W2045556022 countsByYear W20455560222015 @default.
• W2045556022 countsByYear W20455560222016 @default.
• W2045556022 countsByYear W20455560222017 @default.
• W2045556022 countsByYear W20455560222018 @default.
• W2045556022 countsByYear W20455560222019 @default.
• W2045556022 countsByYear W20455560222020 @default.
• W2045556022 countsByYear W20455560222021 @default.
• W2045556022 countsByYear W20455560222022 @default.
• W2045556022 countsByYear W20455560222023 @default.
• W2045556022 crossrefType "journal-article" @default.
• W2045556022 hasAuthorship W2045556022A5007213781 @default.
• W2045556022 hasAuthorship W2045556022A5019629089 @default.
• W2045556022 hasAuthorship W2045556022A5025433608 @default.
• W2045556022 hasAuthorship W2045556022A5034249208 @default.
• W2045556022 hasAuthorship W2045556022A5059177152 @default.
• W2045556022 hasBestOaLocation W20455560221 @default.
• W2045556022 hasConcept C104317684 @default.
• W2045556022 hasConcept C12554922 @default.
• W2045556022 hasConcept C143065580 @default.
• W2045556022 hasConcept C169760540 @default.
• W2045556022 hasConcept C185592680 @default.
• W2045556022 hasConcept C189542057 @default.
• W2045556022 hasConcept C200206546 @default.
• W2045556022 hasConcept C2776580309 @default.
• W2045556022 hasConcept C2777727519 @default.
• W2045556022 hasConcept C37218471 @default.
• W2045556022 hasConcept C41625074 @default.
• W2045556022 hasConcept C50738837 @default.

• next