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• W2045557931 endingPage "R1626" @default.
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• W2045557931 abstract "The mRNA expression of myogenic regulatory factors, including myoD1 (myoblast determination factor) gene paralogs, and their regulation by amino acids and insulin-like growth factors were investigated in primary cell cultures isolated from fast myotomal muscle of Atlantic salmon ( Salmo salar). The cell cycle and S phase were determined as 28.1 and 13.3 h, respectively, at 18°C. Expression of myoD1b and myoD1c peaked at 8 days of culture in the initial proliferation phase and then declined more than sixfold as cells differentiated and was correlated with PCNA (proliferating cell nuclear antigen) expression ( R = 0.88, P < 0.0001; R = 0.70, P < 0.0001). In contrast, myoD1a transcripts increased from 2 to 8 days and remained at elevated levels as myotubes were formed. mRNA levels of myoD1c were, on average, 3.1- and 5.7-fold higher than myoD1a and myoD1b, respectively. Depriving cells of amino acids and serum led to a rapid increase in pax7 and a decrease in myoD1c and PCNA expression, indicating a transition to a quiescent state. In contrast, amino acid replacement in starved cells produced significant increases in myoD1c (at 6 h), PCNA (at 12 h), and myoD1b (at 24 h) and decreases in pax7 expression as cells entered the cell cycle. Our results are consistent with temporally distinct patterns of myoD1c and myoD1b expression at the G 1 and S/G 2 phases of the cell cycle. Treatment of starved cells with insulin-like growth factor I or II did not alter expression of the myoD paralogs. It was concluded that, in vitro, amino acids alone are sufficient to stimulate expression of genes regulating myogenesis in myoblasts involving autocrine/paracrine pathways. The differential responses of myoD paralogs during myotube maturation and amino acid treatments suggest that myoD1b and myoD1c are primarily expressed in proliferating cells and myoD1a in differentiating cells, providing evidence for their subfunctionalization following whole genome and local duplications in the Atlantic salmon lineage." @default.
• W2045557931 created "2016-06-24" @default.
• W2045557931 creator A5023506504 @default.
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• W2045557931 date "2010-06-01" @default.
• W2045557931 modified "2023-10-01" @default.
• W2045557931 title "Paralogs of Atlantic salmon myoblast determination factor genes are distinctly regulated in proliferating and differentiating myogenic cells" @default.
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• W2045557931 cites W1533583970 @default.
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• W2045557931 cites W1837450065 @default.
• W2045557931 cites W1944717769 @default.
• W2045557931 cites W1963605665 @default.
• W2045557931 cites W1964733862 @default.
• W2045557931 cites W1966387132 @default.
• W2045557931 cites W1969650180 @default.
• W2045557931 cites W1969655939 @default.
• W2045557931 cites W1972527462 @default.
• W2045557931 cites W1973217256 @default.
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• W2045557931 cites W1986850329 @default.
• W2045557931 cites W1987030748 @default.
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• W2045557931 cites W1994206543 @default.
• W2045557931 cites W1996015314 @default.
• W2045557931 cites W2004751759 @default.
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• W2045557931 cites W2017429680 @default.
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• W2045557931 cites W2080592158 @default.
• W2045557931 cites W2087803244 @default.
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• W2045557931 cites W2123499039 @default.
• W2045557931 cites W2124479860 @default.
• W2045557931 cites W2124720925 @default.
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• W2045557931 cites W2128940002 @default.
• W2045557931 cites W2132189390 @default.
• W2045557931 cites W2134253526 @default.
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• W2045557931 cites W2139176638 @default.
• W2045557931 cites W2139352935 @default.
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• W2045557931 doi "https://doi.org/10.1152/ajpregu.00114.2010" @default.
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• W2045557931 hasPublicationYear "2010" @default.
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