FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2045561656> ?p ?o ?g. }

• W2045561656 endingPage "5488" @default.
• W2045561656 startingPage "5488" @default.
• W2045561656 abstract "To investigate the immune response of human conjunctival epithelium to hyperosmolar stress.Tear osmolarity was measured in 15 normal subjects and 25 dry eye (DE) patients; conjunctival imprint cytology samples were obtained at the nasal bulbar area. Subconfluent primary human conjunctival epithelial cells (pHCECs) and human conjunctival organ cultures (hCOCs), both cultured in iso-osmolar medium (305 mOsm/L), were exposed for 24 hours to media with progressively higher osmolarity, with or without the ion channel inhibitor ruthenium red (RuR). Human leukocyte antigen (HLA)-DR expression was evaluated by immunocytochemistry, on imprints from subjects, on primary human conjunctival epithelial cells, on formalin fixed-paraffin embedded hCOCs, and by RT-PCR. Statistical evaluation was performed by applying the unpaired Student's t test, as well as Spearman's rho and Pearson's r correlation coefficients (significance P < 0.05).HLA-DR expression increased in DE subjects with respect to control (% mean ± SD, respectively, 46.16 ± 7.2 vs. 7.48 ± 1.14, P < 0.0001) and exhibited significantly high correlations with tear osmolarity values (r = 0.614; P < 0.0001). In vitro experiments showed a progressive increase in HLA-DR expression as the osmolarity of the medium was increased from 6.75 ± 1.16 (% mean ± SD) in iso-osmolar-cultured cells to 9.96 ± 1.37 and 12.94 ± 4.04 in cells cultured in, respectively, 350 and 400 mOsm/L (P < 0.05). A stepwise progressive increase was also found in hCOCs. Results were confirmed by RT-PCR. Ruthenium red significantly reduced HLA-DR expression in hyperosmolar-cultured cells.Data from complementary techniques demonstrate that extracellular hyperosmolarity induces HLA-DR overexpression in human conjunctival epithelial cells in both DE patients and in vitro cell culture models." @default.
• W2045561656 created "2016-06-24" @default.
• W2045561656 creator A5006038965 @default.
• W2045561656 creator A5065530477 @default.
• W2045561656 creator A5074759909 @default.
• W2045561656 creator A5091399810 @default.
• W2045561656 date "2011-07-25" @default.
• W2045561656 modified "2023-10-16" @default.
• W2045561656 title "Hyperosmolar Stress Upregulates HLA-DR Expression in Human Conjunctival Epithelium in Dry Eye Patients and In Vitro Models" @default.
• W2045561656 cites W1542814291 @default.
• W2045561656 cites W1574301155 @default.
• W2045561656 cites W159017383 @default.
• W2045561656 cites W1594083439 @default.
• W2045561656 cites W168424518 @default.
• W2045561656 cites W1966308680 @default.
• W2045561656 cites W1967999929 @default.
• W2045561656 cites W1970531479 @default.
• W2045561656 cites W1974540299 @default.
• W2045561656 cites W1985634053 @default.
• W2045561656 cites W2005273232 @default.
• W2045561656 cites W2008878337 @default.
• W2045561656 cites W2009276059 @default.
• W2045561656 cites W2010233517 @default.
• W2045561656 cites W2012144205 @default.
• W2045561656 cites W2013268532 @default.
• W2045561656 cites W2015494251 @default.
• W2045561656 cites W2021968547 @default.
• W2045561656 cites W2024921065 @default.
• W2045561656 cites W2025829142 @default.
• W2045561656 cites W2029515932 @default.
• W2045561656 cites W2031036631 @default.
• W2045561656 cites W2031502502 @default.
• W2045561656 cites W2033650789 @default.
• W2045561656 cites W2039751095 @default.
• W2045561656 cites W2045533747 @default.
• W2045561656 cites W2054013327 @default.
• W2045561656 cites W2055038354 @default.
• W2045561656 cites W2073779619 @default.
• W2045561656 cites W2075995435 @default.
• W2045561656 cites W2083394710 @default.
• W2045561656 cites W2086358431 @default.
• W2045561656 cites W2088675292 @default.
• W2045561656 cites W2091630088 @default.
• W2045561656 cites W2091662896 @default.
• W2045561656 cites W2092613545 @default.
• W2045561656 cites W2095815384 @default.
• W2045561656 cites W2097824035 @default.
• W2045561656 cites W2098089241 @default.
• W2045561656 cites W2101285435 @default.
• W2045561656 cites W2108244474 @default.
• W2045561656 cites W2124600315 @default.
• W2045561656 cites W2129020034 @default.
• W2045561656 cites W2129830339 @default.
• W2045561656 cites W2136473116 @default.
• W2045561656 cites W2141860584 @default.
• W2045561656 cites W2159681472 @default.
• W2045561656 cites W2159927241 @default.
• W2045561656 cites W2596391620 @default.
• W2045561656 cites W2598546209 @default.
• W2045561656 cites W2888672724 @default.
• W2045561656 doi "https://doi.org/10.1167/iovs.11-7215" @default.
• W2045561656 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21498621" @default.
• W2045561656 hasPublicationYear "2011" @default.
• W2045561656 type Work @default.
• W2045561656 sameAs 2045561656 @default.
• W2045561656 citedByCount "53" @default.
• W2045561656 countsByYear W20455616562012 @default.
• W2045561656 countsByYear W20455616562013 @default.
• W2045561656 countsByYear W20455616562014 @default.
• W2045561656 countsByYear W20455616562015 @default.
• W2045561656 countsByYear W20455616562016 @default.
• W2045561656 countsByYear W20455616562017 @default.
• W2045561656 countsByYear W20455616562018 @default.
• W2045561656 countsByYear W20455616562019 @default.
• W2045561656 countsByYear W20455616562020 @default.
• W2045561656 countsByYear W20455616562021 @default.
• W2045561656 countsByYear W20455616562022 @default.
• W2045561656 countsByYear W20455616562023 @default.
• W2045561656 crossrefType "journal-article" @default.
• W2045561656 hasAuthorship W2045561656A5006038965 @default.
• W2045561656 hasAuthorship W2045561656A5065530477 @default.
• W2045561656 hasAuthorship W2045561656A5074759909 @default.
• W2045561656 hasAuthorship W2045561656A5091399810 @default.
• W2045561656 hasBestOaLocation W20455616561 @default.
• W2045561656 hasConcept C118487528 @default.
• W2045561656 hasConcept C126322002 @default.
• W2045561656 hasConcept C129900496 @default.
• W2045561656 hasConcept C142724271 @default.
• W2045561656 hasConcept C14705128 @default.
• W2045561656 hasConcept C147483822 @default.
• W2045561656 hasConcept C153911025 @default.
• W2045561656 hasConcept C16685009 @default.
• W2045561656 hasConcept C18823058 @default.
• W2045561656 hasConcept C188280979 @default.
• W2045561656 hasConcept C202751555 @default.
• W2045561656 hasConcept C203014093 @default.
• W2045561656 hasConcept C2775984611 @default.
• W2045561656 hasConcept C35866371 @default.

• next