FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2045579951> ?p ?o ?g. }

• W2045579951 endingPage "4710" @default.
• W2045579951 startingPage "4703" @default.
• W2045579951 abstract "Evidencing subtle conformational transitions in proteins occurring upon small modulator binding usually requires atomic resolution techniques (X-ray crystallography or NMR). Recently, hyphenation of ion mobility and mass spectrometry (IM-MS) has greatly enlarged the potentials for biomolecular assembly structural characterization. Using the well 3D-characterized Bcl-xL/ABT-737 protein model, we explored in the present report whether IM-MS can be used to differentiate close conformers and monitor collision cross section (CCS) differences correlating with ligand-induced conformational changes. Because comparing CCS derived from IM-MS data with 3D-computed CCS is critical for thorough data interpretation, discussing pitfalls related to protein construct similarity and missing sequence sections in PDB files was of primary importance to avoid misinterpretation. The methodic exploration of instrument parameters showed enhanced IM separation of Bcl-xL conformers by combining high wave heights and velocities with low helium and nitrogen flow rates while keeping a high He/N2 flow rate ratio (>3). The robustness of CCS measurements was eventually improved with a modified IM calibration method providing constant CCS values regardless of instrument settings. Altogether, optimized IM-MS settings allowed a 0.4 nm2 increase (i.e., 2%) of Bcl-xL CCS to be evidenced upon ABT-737 binding." @default.
• W2045579951 created "2016-06-24" @default.
• W2045579951 creator A5010920048 @default.
• W2045579951 creator A5023284045 @default.
• W2045579951 creator A5041974331 @default.
• W2045579951 creator A5060217867 @default.
• W2045579951 creator A5067605957 @default.
• W2045579951 creator A5073068916 @default.
• W2045579951 date "2012-06-05" @default.
• W2045579951 modified "2023-09-30" @default.
• W2045579951 title "Exploring Key Parameters to Detect Subtle Ligand-Induced Protein Conformational Changes Using Traveling Wave Ion Mobility Mass Spectrometry" @default.
• W2045579951 cites W1964651116 @default.
• W2045579951 cites W1964918396 @default.
• W2045579951 cites W1965217295 @default.
• W2045579951 cites W1969242164 @default.
• W2045579951 cites W1972539512 @default.
• W2045579951 cites W1974959933 @default.
• W2045579951 cites W1988308498 @default.
• W2045579951 cites W1991437941 @default.
• W2045579951 cites W1994494141 @default.
• W2045579951 cites W2009840402 @default.
• W2045579951 cites W2011736771 @default.
• W2045579951 cites W2019812098 @default.
• W2045579951 cites W2023993152 @default.
• W2045579951 cites W2025403616 @default.
• W2045579951 cites W2030175020 @default.
• W2045579951 cites W2035123840 @default.
• W2045579951 cites W2042085007 @default.
• W2045579951 cites W2042119692 @default.
• W2045579951 cites W2049172807 @default.
• W2045579951 cites W2054083856 @default.
• W2045579951 cites W2055106056 @default.
• W2045579951 cites W2057020790 @default.
• W2045579951 cites W2071884490 @default.
• W2045579951 cites W2076451815 @default.
• W2045579951 cites W2091474459 @default.
• W2045579951 cites W2108112365 @default.
• W2045579951 cites W2115569106 @default.
• W2045579951 cites W2115588498 @default.
• W2045579951 cites W2137836801 @default.
• W2045579951 cites W2143694555 @default.
• W2045579951 cites W2153946781 @default.
• W2045579951 cites W2230658567 @default.
• W2045579951 cites W2317233863 @default.
• W2045579951 cites W2951997582 @default.
• W2045579951 doi "https://doi.org/10.1021/ac203223h" @default.
• W2045579951 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22533353" @default.
• W2045579951 hasPublicationYear "2012" @default.
• W2045579951 type Work @default.
• W2045579951 sameAs 2045579951 @default.
• W2045579951 citedByCount "26" @default.
• W2045579951 countsByYear W20455799512012 @default.
• W2045579951 countsByYear W20455799512013 @default.
• W2045579951 countsByYear W20455799512014 @default.
• W2045579951 countsByYear W20455799512015 @default.
• W2045579951 countsByYear W20455799512016 @default.
• W2045579951 countsByYear W20455799512017 @default.
• W2045579951 countsByYear W20455799512018 @default.
• W2045579951 countsByYear W20455799512019 @default.
• W2045579951 countsByYear W20455799512020 @default.
• W2045579951 countsByYear W20455799512021 @default.
• W2045579951 countsByYear W20455799512022 @default.
• W2045579951 countsByYear W20455799512023 @default.
• W2045579951 crossrefType "journal-article" @default.
• W2045579951 hasAuthorship W2045579951A5010920048 @default.
• W2045579951 hasAuthorship W2045579951A5023284045 @default.
• W2045579951 hasAuthorship W2045579951A5041974331 @default.
• W2045579951 hasAuthorship W2045579951A5060217867 @default.
• W2045579951 hasAuthorship W2045579951A5067605957 @default.
• W2045579951 hasAuthorship W2045579951A5073068916 @default.
• W2045579951 hasConcept C113196181 @default.
• W2045579951 hasConcept C127313418 @default.
• W2045579951 hasConcept C15213710 @default.
• W2045579951 hasConcept C162356407 @default.
• W2045579951 hasConcept C178790620 @default.
• W2045579951 hasConcept C185592680 @default.
• W2045579951 hasConcept C18705241 @default.
• W2045579951 hasConcept C3020199158 @default.
• W2045579951 hasConcept C32909587 @default.
• W2045579951 hasConcept C43617362 @default.
• W2045579951 hasConcept C62649853 @default.
• W2045579951 hasConcept C65556437 @default.
• W2045579951 hasConcept C71240020 @default.
• W2045579951 hasConcept C8010536 @default.
• W2045579951 hasConceptScore W2045579951C113196181 @default.
• W2045579951 hasConceptScore W2045579951C127313418 @default.
• W2045579951 hasConceptScore W2045579951C15213710 @default.
• W2045579951 hasConceptScore W2045579951C162356407 @default.
• W2045579951 hasConceptScore W2045579951C178790620 @default.
• W2045579951 hasConceptScore W2045579951C185592680 @default.
• W2045579951 hasConceptScore W2045579951C18705241 @default.
• W2045579951 hasConceptScore W2045579951C3020199158 @default.
• W2045579951 hasConceptScore W2045579951C32909587 @default.
• W2045579951 hasConceptScore W2045579951C43617362 @default.
• W2045579951 hasConceptScore W2045579951C62649853 @default.
• W2045579951 hasConceptScore W2045579951C65556437 @default.
• W2045579951 hasConceptScore W2045579951C71240020 @default.
• W2045579951 hasConceptScore W2045579951C8010536 @default.

• next