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• W2045602956 abstract "We have developed a highly accurate and sensitive real-time polymerase chain reaction (PCR) assay to detect and quantify Leishmania parasites. The assay targets GP63, a highly conserved gene in Leishmania. We demonstrate that, with a single assay, we are able to detect and quantify several species of Leishmania. Our assay system detects Leishmania donovani and Leishmania major down to 0.1 parasite. The dynamic range of the assay extends over 6 log cycles of target, with an average correlation coefficient >0.988. In addition, we utilize a simple approach to distinguish between Leishmania species causing diverse spectra of disease. We have also used this assay to follow the course of cutaneous disease in CBA/CaJ mice, known to be resistant to L. major. The assay is sensitive enough to quantify parasite load in the absence of overt lesions and reveals a systemic distribution of Leishmania, which has implications for our understanding of the disease." @default.
• W2045602956 created "2016-06-24" @default.
• W2045602956 creator A5011472628 @default.
• W2045602956 creator A5015959917 @default.
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• W2045602956 date "2008-05-01" @default.
• W2045602956 modified "2023-09-26" @default.
• W2045602956 title "Development of a real-time polymerase chain reaction assay for the quantification of Leishmania species and the monitoring of systemic distribution of the pathogen" @default.
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• W2045602956 doi "https://doi.org/10.1016/j.diagmicrobio.2007.12.013" @default.
• W2045602956 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18255247" @default.
• W2045602956 hasPublicationYear "2008" @default.
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