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- W2045603364 abstract "The geneticdiversity and the potential toxicity of bloom-forming cyanobacteria were studiedin four lagoons located in the state of Sao Paulo (Campinas, Limeira and Piracicabacities). Bloom samples were collected on the water surface and cyanobacterial communitieswere evaluated using DGGE fingerprinting and 16S rDNA clone library. The amplificationof genes encoding secondary metabolites such as microcystin (mcy), anatoxin (ana), cylindrospermopsin (cyr), saxitoxin (sxt), cyanopeptolin (mcn) and aeruginosin (aer) was performedand their production analyzed by LC-MS. The comparison of DGGE banding pattern amongthe different water samples suggested that some operational taxonomic units (OTUs)in these locations were predominant over others. The 16S rDNA clone libraries sequencesmatched with nine different known cyanobacterial genera available in NCBI, identifiedas Anabaena, Brasilonema, Cylindrospermopsis, Limnococcus, Microcystis, Nostoc, Pseudanabaena, Synechococcus and Woronichinia.The lagoons ESALQ2, Taquaral and Limeira had more than 80% of the cyanobacterialcommunity assigned to the genus Microcystis.Genes encoding aeruginosin, cyanopeptolin and microcystin synthetases and saxitoxinsynthase were amplified, and LC-MS/MS confirmed the production of aeruginosin, cyanopeptolinand microcystin. Rapid and sensitive methods for the detection of these secondarymetabolites, especially toxins, using chemical and molecular tools together, canbe used for a faster diagnostic of toxic cyanobacterial blooms." @default.
- W2045603364 created "2016-06-24" @default.
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- W2045603364 date "2015-01-01" @default.
- W2045603364 modified "2023-10-06" @default.
- W2045603364 title "Molecular and Chemical Analyses of Cyanobacterial Blooms in Tropical Lagoons from Southeast Brazil" @default.
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- W2045603364 doi "https://doi.org/10.4236/jwarp.2015.71004" @default.
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