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- W2045608399 abstract "Strains of Verticillium albo-atrum Reinke & Berth, from tomato and hop were shown to produce a chitinase in pure culture capable of degrading chitin to N-acetylglucosamine. Shake and fermentor culture filtrates showed pronounced chitinase activity during the log phase which paralleled changes in dry weight. The enzyme was detected in sonicated mycelium preparations and produced N-acetylglucosamine when incubated with autoclaved, washed Verticillium hyphae. The enzyme was active between pH 4·5 and pH 6·0 with a maximum at pH 5·1, and was active between 12° and 60° with an optimum at 45°. Inhibitor studies showed identical responses between the chitinases from Verticillium and Streptomyces. Potassium cyanide was strongly inhibitory to both enzymes but the respiratory inhibitor sodium azide at 0·5 m concentration was non-inhibitory. The results are discussed briefly in relation to the lysis of mycelium in infected tomato plants." @default.
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- W2045608399 date "1973-02-01" @default.
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- W2045608399 title "Autolysis and chitinase production in cultures of Verticillium albo-atrum" @default.
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- W2045608399 doi "https://doi.org/10.1016/s0007-1536(73)80068-3" @default.
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