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• W2045615087 abstract "Recent studies show that classical swine fever virus (CSFV) NS5A is an essential replicase component, but it is not known how NS5A participates in viral particle production. In this study, deletion and substitution mutations were introduced into the C-terminus of CSFV NS5A. The efficiency of Core protein release and extracellular and intracellular infectivity levels were assessed and NS5A–Core interaction was investigated. These results suggested that CSFV NS5A was a key factor for the assembly of infectious CSFV particles. The C-terminal sequence from amino acids 478 to 487 and amino acids S481 and T482 were necessary for CSFV assembly and production. The effect of NS5A on CSFV assembly and production might be related to NS5A–Core interaction. T482 was found to be conserved in the C-terminus of NS5A proteins of pestiviruses and hepatitis C virus (HCV), therefore suggesting that it might be important for these virus assembly and production." @default.
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• W2045615087 date "2014-10-01" @default.
• W2045615087 modified "2023-10-14" @default.
• W2045615087 title "Characterization of the C-terminal sequence of NS5A necessary for the assembly and production of classical swine fever virus infectious particles" @default.
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• W2045615087 doi "https://doi.org/10.1016/j.rvsc.2014.07.017" @default.
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