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• W2045642792 abstract "A novel label-free fluorescence recovery assay for the sensing of a DNA binding protein has been developed. A transcription factor c-Jun protein, and a 21 base pair duplex DNA containing the c-Jun protein binding site (J-DNA) were selected. J-DNA was mixed with a cationic fluorescent perylene probe (compound 1), and induced aggregation of the probe. Quenching of the probe's fluorescence was observed. However, when c-Jun protein was mixed with the J-DNA, c-Jun bound to the duplex DNA, which reduced the degree of the induced perylene probe aggregation, and a turn on fluorescence signal was observed. The recovered fluorescence intensity was directly related to the amount of c-Jun added. The method is highly selective, six non-DNA binding proteins and one randomly selected 21 base pair duplex DNA (con-1) were tested. No noticeable compound 1 fluorescence recovery was observed. Mutations were also introduced to the c-Jun recognition sequence and much reduced fluorescence recovery was observed. Our assay is label-free, convenient, inexpensive, and fast. It can be used in biomedical research such as high throughput screening of drugs targeted at DNA-binding proteins." @default.
• W2045642792 created "2016-06-24" @default.
• W2045642792 creator A5026928688 @default.
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• W2045642792 date "2013-10-01" @default.
• W2045642792 modified "2023-09-27" @default.
• W2045642792 title "Sensing of a nucleic acid binding protein via a label-free perylene probe fluorescence recovery assay" @default.
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• W2045642792 doi "https://doi.org/10.1016/j.aca.2013.08.022" @default.
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