Matches in SemOpenAlex for { <https://semopenalex.org/work/W2045646223> ?p ?o ?g. }
- W2045646223 endingPage "e1002739" @default.
- W2045646223 startingPage "e1002739" @default.
- W2045646223 abstract "During cell division, segregation of sister chromatids to daughter cells is achieved by the poleward pulling force of microtubules, which attach to the chromatids by means of a multiprotein complex, the kinetochore. Kinetochores assemble at the centromeric DNA organized by specialized centromeric nucleosomes. In contrast to other eukaryotes, which typically have large repetitive centromeric regions, budding yeast CEN DNA is defined by a 125 bp sequence and assembles a single centromeric nucleosome. In budding yeast, as well as in other eukaryotes, the Cse4 histone variant (known in vertebrates as CENP-A) is believed to substitute for histone H3 at the centromeric nucleosome. However, the exact composition of the CEN nucleosome remains a subject of debate. We report the use of a novel ChIP approach to reveal the composition of the centromeric nucleosome and its localization on CEN DNA in budding yeast. Surprisingly, we observed a strong interaction of H3, as well as Cse4, H4, H2A, and H2B, but not histone chaperone Scm3 (HJURP in human) with the centromeric DNA. H3 localizes to centromeric DNA at all stages of the cell cycle. Using a sequential ChIP approach, we could demonstrate the co-occupancy of H3 and Cse4 at the CEN DNA. Our results favor a H3-Cse4 heterotypic octamer at the budding yeast centromere. Whether or not our model is correct, any future model will have to account for the stable association of histone H3 with the centromeric DNA." @default.
- W2045646223 created "2016-06-24" @default.
- W2045646223 creator A5038705084 @default.
- W2045646223 creator A5062608849 @default.
- W2045646223 date "2012-05-31" @default.
- W2045646223 modified "2023-09-25" @default.
- W2045646223 title "Histone H3 Localizes to the Centromeric DNA in Budding Yeast" @default.
- W2045646223 cites W1832254521 @default.
- W2045646223 cites W1909790672 @default.
- W2045646223 cites W1969978677 @default.
- W2045646223 cites W1970549330 @default.
- W2045646223 cites W1970668361 @default.
- W2045646223 cites W1971651847 @default.
- W2045646223 cites W1994181100 @default.
- W2045646223 cites W1994634104 @default.
- W2045646223 cites W1995275121 @default.
- W2045646223 cites W1997394805 @default.
- W2045646223 cites W1999215733 @default.
- W2045646223 cites W2003345607 @default.
- W2045646223 cites W2004910827 @default.
- W2045646223 cites W2005880821 @default.
- W2045646223 cites W2010595180 @default.
- W2045646223 cites W2013711216 @default.
- W2045646223 cites W2017165841 @default.
- W2045646223 cites W2019180618 @default.
- W2045646223 cites W2020599848 @default.
- W2045646223 cites W2026304022 @default.
- W2045646223 cites W2033328077 @default.
- W2045646223 cites W2038648745 @default.
- W2045646223 cites W2038870980 @default.
- W2045646223 cites W2040904248 @default.
- W2045646223 cites W2041870135 @default.
- W2045646223 cites W2042450235 @default.
- W2045646223 cites W2044219999 @default.
- W2045646223 cites W2045415591 @default.
- W2045646223 cites W2049912093 @default.
- W2045646223 cites W2053789874 @default.
- W2045646223 cites W2060073273 @default.
- W2045646223 cites W2061401851 @default.
- W2045646223 cites W2066173190 @default.
- W2045646223 cites W2068097279 @default.
- W2045646223 cites W2068608369 @default.
- W2045646223 cites W2071391879 @default.
- W2045646223 cites W2073903736 @default.
- W2045646223 cites W2074908472 @default.
- W2045646223 cites W2080457024 @default.
- W2045646223 cites W2087712265 @default.
- W2045646223 cites W2088929283 @default.
- W2045646223 cites W2092273279 @default.
- W2045646223 cites W2097363543 @default.
- W2045646223 cites W2104481217 @default.
- W2045646223 cites W2105178351 @default.
- W2045646223 cites W2110581419 @default.
- W2045646223 cites W2115031550 @default.
- W2045646223 cites W2117305444 @default.
- W2045646223 cites W2125415070 @default.
- W2045646223 cites W2129617454 @default.
- W2045646223 cites W2131633518 @default.
- W2045646223 cites W2137435947 @default.
- W2045646223 cites W2141097743 @default.
- W2045646223 cites W2144533535 @default.
- W2045646223 cites W2144792857 @default.
- W2045646223 cites W2146087376 @default.
- W2045646223 cites W2151177136 @default.
- W2045646223 cites W2155235554 @default.
- W2045646223 cites W2156773083 @default.
- W2045646223 cites W2161938609 @default.
- W2045646223 cites W2170123451 @default.
- W2045646223 doi "https://doi.org/10.1371/journal.pgen.1002739" @default.
- W2045646223 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3364953" @default.
- W2045646223 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22693454" @default.
- W2045646223 hasPublicationYear "2012" @default.
- W2045646223 type Work @default.
- W2045646223 sameAs 2045646223 @default.
- W2045646223 citedByCount "17" @default.
- W2045646223 countsByYear W20456462232012 @default.
- W2045646223 countsByYear W20456462232013 @default.
- W2045646223 countsByYear W20456462232014 @default.
- W2045646223 countsByYear W20456462232015 @default.
- W2045646223 countsByYear W20456462232016 @default.
- W2045646223 countsByYear W20456462232017 @default.
- W2045646223 countsByYear W20456462232018 @default.
- W2045646223 countsByYear W20456462232019 @default.
- W2045646223 countsByYear W20456462232020 @default.
- W2045646223 crossrefType "journal-article" @default.
- W2045646223 hasAuthorship W2045646223A5038705084 @default.
- W2045646223 hasAuthorship W2045646223A5062608849 @default.
- W2045646223 hasBestOaLocation W20456462231 @default.
- W2045646223 hasConcept C10058791 @default.
- W2045646223 hasConcept C104317684 @default.
- W2045646223 hasConcept C150194340 @default.
- W2045646223 hasConcept C173681060 @default.
- W2045646223 hasConcept C190727270 @default.
- W2045646223 hasConcept C192838845 @default.
- W2045646223 hasConcept C22709980 @default.
- W2045646223 hasConcept C2776745794 @default.
- W2045646223 hasConcept C30481170 @default.
- W2045646223 hasConcept C54355233 @default.