FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2045647323> ?p ?o ?g. }

• W2045647323 endingPage "79" @default.
• W2045647323 startingPage "74" @default.
• W2045647323 abstract "Macrobrachium rosenbergii nodavirus (MrNv) infects giant freshwater prawns and causes white tail disease (WTD). The coding region of the capsid protein of MrNv was amplified with RT-PCR and cloned into the pTrcHis2-TOPO vector. The recombinant plasmid was introduced into Escherichia coli and protein expression was induced with IPTG. SDS-PAGE showed that the recombinant protein containing the His-tag and myc epitope has a molecular mass of about 46 kDa and it was detected by the anti-His antibody in Western blotting. The protein was purified using immobilized metal affinity chromatography (IMAC) and transmission electron microscopic analysis revealed that the recombinant protein assembled into virus-like particles (VLPs) with a diameter of about 30±3 nm. The size of the particles was confirmed by dynamic light scattering. Nucleic acids were extracted from the VLPs and treatment with nucleases showed that they were mainly RNA molecules. This is the first report describing the production of MrNv capsid protein in bacteria and its assembly into VLPs." @default.
• W2045647323 created "2016-06-24" @default.
• W2045647323 creator A5042948091 @default.
• W2045647323 creator A5070989264 @default.
• W2045647323 creator A5088463194 @default.
• W2045647323 date "2011-07-01" @default.
• W2045647323 modified "2023-09-26" @default.
• W2045647323 title "Virus-like particles of Macrobrachium rosenbergii nodavirus produced in bacteria" @default.
• W2045647323 cites W1569161839 @default.
• W2045647323 cites W1834124020 @default.
• W2045647323 cites W1964742954 @default.
• W2045647323 cites W1972964821 @default.
• W2045647323 cites W2009144084 @default.
• W2045647323 cites W2016679429 @default.
• W2045647323 cites W2041649294 @default.
• W2045647323 cites W2058081782 @default.
• W2045647323 cites W2074902438 @default.
• W2045647323 cites W2089338153 @default.
• W2045647323 cites W2101044923 @default.
• W2045647323 cites W2116557030 @default.
• W2045647323 cites W2138022492 @default.
• W2045647323 cites W2140748317 @default.
• W2045647323 cites W2153731352 @default.
• W2045647323 cites W4293247451 @default.
• W2045647323 cites W2001662797 @default.
• W2045647323 doi "https://doi.org/10.1016/j.jviromet.2011.04.021" @default.
• W2045647323 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21536072" @default.
• W2045647323 hasPublicationYear "2011" @default.
• W2045647323 type Work @default.
• W2045647323 sameAs 2045647323 @default.
• W2045647323 citedByCount "38" @default.
• W2045647323 countsByYear W20456473232014 @default.
• W2045647323 countsByYear W20456473232015 @default.
• W2045647323 countsByYear W20456473232016 @default.
• W2045647323 countsByYear W20456473232017 @default.
• W2045647323 countsByYear W20456473232018 @default.
• W2045647323 countsByYear W20456473232019 @default.
• W2045647323 countsByYear W20456473232020 @default.
• W2045647323 countsByYear W20456473232021 @default.
• W2045647323 countsByYear W20456473232022 @default.
• W2045647323 countsByYear W20456473232023 @default.
• W2045647323 crossrefType "journal-article" @default.
• W2045647323 hasAuthorship W2045647323A5042948091 @default.
• W2045647323 hasAuthorship W2045647323A5070989264 @default.
• W2045647323 hasAuthorship W2045647323A5088463194 @default.
• W2045647323 hasBestOaLocation W20456473232 @default.
• W2045647323 hasConcept C104317684 @default.
• W2045647323 hasConcept C153911025 @default.
• W2045647323 hasConcept C159047783 @default.
• W2045647323 hasConcept C170344550 @default.
• W2045647323 hasConcept C181199279 @default.
• W2045647323 hasConcept C202878990 @default.
• W2045647323 hasConcept C22744801 @default.
• W2045647323 hasConcept C24107716 @default.
• W2045647323 hasConcept C2522874641 @default.
• W2045647323 hasConcept C2775995008 @default.
• W2045647323 hasConcept C2776115401 @default.
• W2045647323 hasConcept C2776387124 @default.
• W2045647323 hasConcept C2779771762 @default.
• W2045647323 hasConcept C33161422 @default.
• W2045647323 hasConcept C40767141 @default.
• W2045647323 hasConcept C505870484 @default.
• W2045647323 hasConcept C547475151 @default.
• W2045647323 hasConcept C55493867 @default.
• W2045647323 hasConcept C58121356 @default.
• W2045647323 hasConcept C86803240 @default.
• W2045647323 hasConceptScore W2045647323C104317684 @default.
• W2045647323 hasConceptScore W2045647323C153911025 @default.
• W2045647323 hasConceptScore W2045647323C159047783 @default.
• W2045647323 hasConceptScore W2045647323C170344550 @default.
• W2045647323 hasConceptScore W2045647323C181199279 @default.
• W2045647323 hasConceptScore W2045647323C202878990 @default.
• W2045647323 hasConceptScore W2045647323C22744801 @default.
• W2045647323 hasConceptScore W2045647323C24107716 @default.
• W2045647323 hasConceptScore W2045647323C2522874641 @default.
• W2045647323 hasConceptScore W2045647323C2775995008 @default.
• W2045647323 hasConceptScore W2045647323C2776115401 @default.
• W2045647323 hasConceptScore W2045647323C2776387124 @default.
• W2045647323 hasConceptScore W2045647323C2779771762 @default.
• W2045647323 hasConceptScore W2045647323C33161422 @default.
• W2045647323 hasConceptScore W2045647323C40767141 @default.
• W2045647323 hasConceptScore W2045647323C505870484 @default.
• W2045647323 hasConceptScore W2045647323C547475151 @default.
• W2045647323 hasConceptScore W2045647323C55493867 @default.
• W2045647323 hasConceptScore W2045647323C58121356 @default.
• W2045647323 hasConceptScore W2045647323C86803240 @default.
• W2045647323 hasIssue "1" @default.
• W2045647323 hasLocation W20456473231 @default.
• W2045647323 hasLocation W20456473232 @default.
• W2045647323 hasLocation W20456473233 @default.
• W2045647323 hasOpenAccess W2045647323 @default.
• W2045647323 hasPrimaryLocation W20456473231 @default.
• W2045647323 hasRelatedWork W2045647323 @default.
• W2045647323 hasRelatedWork W2083386540 @default.
• W2045647323 hasRelatedWork W2351989966 @default.
• W2045647323 hasRelatedWork W2355058011 @default.
• W2045647323 hasRelatedWork W2356201388 @default.
• W2045647323 hasRelatedWork W2373646174 @default.

• next