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- W204567168 abstract "Publisher SummaryThis chapter describes the effect of nutritional status and growth hormone (GH) treatment on the in vitro mitogenic responses of mammary gland tissue in lambs. Both restricted rates of liveweight gain (LWG) and exogenous GH administration stimulate mammary gland development in prepubertal female lambs. The effects of nutritional status and GH treatment in vivo on the subsequent response of mammary gland expiants to mitogenic stimuli in vitro are studied. Sixteen lambs were randomly allocated to four dietary treatments which includes 12 or 20% crude protein offered at either ad libitum or restricted rates. Within each diet lambs were given daily injections of buffered saline or GH. Treatments were imposed from 9 weeks of age for a period of 10 week. The in vitro mitogenic responses were measured by determining the incorporation of [3H]thymidine into the expiant DNA over the first 24 h of culture in serum-free medium, supplemented with no hormone, insulin or insulin-like growth factor-I. In all treatments, apart from ad libitum feeding, there was a dose-dependent response of DNA synthesis to both I and IGF-I. Basal and stimulated response was greatest in mammary tissue from restricted lambs. Growth hormone treatment and high dietary protein were also associated with enhanced DNA synthesis." @default.
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- W204567168 date "1989-01-01" @default.
- W204567168 modified "2023-09-27" @default.
- W204567168 title "THE EFFECT OF NUTRITIONAL STATUS AND GROWTH HORMONE (GH) TREATMENT ON THE IN VITRO MITOGENIC RESPONSES OF MAMMARY GLAND TISSUE IN LAMBS" @default.
- W204567168 doi "https://doi.org/10.1016/b978-0-407-01473-2.50059-x" @default.
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