Matches in SemOpenAlex for { <https://semopenalex.org/work/W2045773985> ?p ?o ?g. }
- W2045773985 endingPage "6602" @default.
- W2045773985 startingPage "6593" @default.
- W2045773985 abstract "This review takes a second look at a set of mRNAs that purportedly employ an alternative mechanism of initiation when cap-dependent translation is reduced during mitosis or stress conditions. A closer look is necessary because evidence cited in support of the internal initiation hypothesis is often flawed. When putative internal ribosome entry sequences (IRESs) are examined more carefully, they often turn out to harbor cryptic promoters or splice sites. This undermines the dicistronic assay, wherein IRES activity is measured by the ability to support translation of the 3′ cistron. Most putative IRESs still have not been checked carefully to determine whether the dicistronic vector produces only the intended dicistronic mRNA. The widespread use of the pRF vector is a major problem because this vector, which has Renilla luciferase as the 5′ cistron and firefly luciferase as the 3′ cistron, has been found to generate spliced transcripts. RNA transfection assays could theoretically circumvent these problems, but most candidate IRESs score very weakly in that test. The practice of calling even very weak results ‘positive’ is one of the problems discussed herein. The extremely low efficiency of putative IRESs is inconsistent with their postulated biological roles. ‘…if it is a Miracle, any sort of evidence will answer, but if it is a Fact, proof is necessary’ —Mark Twain, Letters from the Earth" @default.
- W2045773985 created "2016-06-24" @default.
- W2045773985 creator A5023781524 @default.
- W2045773985 date "2005-11-27" @default.
- W2045773985 modified "2023-10-10" @default.
- W2045773985 title "A second look at cellular mRNA sequences said to function as internal ribosome entry sites" @default.
- W2045773985 cites W1497600623 @default.
- W2045773985 cites W1578955626 @default.
- W2045773985 cites W1592629267 @default.
- W2045773985 cites W1631020643 @default.
- W2045773985 cites W1846338561 @default.
- W2045773985 cites W1966275499 @default.
- W2045773985 cites W1966734858 @default.
- W2045773985 cites W1967192674 @default.
- W2045773985 cites W1968648015 @default.
- W2045773985 cites W1971140237 @default.
- W2045773985 cites W1979168172 @default.
- W2045773985 cites W1981577767 @default.
- W2045773985 cites W1982763704 @default.
- W2045773985 cites W1982980697 @default.
- W2045773985 cites W1984002397 @default.
- W2045773985 cites W1993036672 @default.
- W2045773985 cites W1993738860 @default.
- W2045773985 cites W1996799677 @default.
- W2045773985 cites W1997874151 @default.
- W2045773985 cites W1999232838 @default.
- W2045773985 cites W2000757618 @default.
- W2045773985 cites W2003241548 @default.
- W2045773985 cites W2004014828 @default.
- W2045773985 cites W2007089586 @default.
- W2045773985 cites W2007793133 @default.
- W2045773985 cites W2008086014 @default.
- W2045773985 cites W2011844607 @default.
- W2045773985 cites W2012597322 @default.
- W2045773985 cites W2017356464 @default.
- W2045773985 cites W2019031253 @default.
- W2045773985 cites W2020659516 @default.
- W2045773985 cites W2021263359 @default.
- W2045773985 cites W2027147867 @default.
- W2045773985 cites W2032411168 @default.
- W2045773985 cites W2032481185 @default.
- W2045773985 cites W2039169535 @default.
- W2045773985 cites W2041122594 @default.
- W2045773985 cites W2042173490 @default.
- W2045773985 cites W2042974023 @default.
- W2045773985 cites W2043256815 @default.
- W2045773985 cites W2046741662 @default.
- W2045773985 cites W2047097142 @default.
- W2045773985 cites W2049661143 @default.
- W2045773985 cites W2052805423 @default.
- W2045773985 cites W2054050246 @default.
- W2045773985 cites W2058438178 @default.
- W2045773985 cites W2068994981 @default.
- W2045773985 cites W2078392491 @default.
- W2045773985 cites W2080515149 @default.
- W2045773985 cites W2081053429 @default.
- W2045773985 cites W2085918490 @default.
- W2045773985 cites W2086988244 @default.
- W2045773985 cites W2087693580 @default.
- W2045773985 cites W2091091730 @default.
- W2045773985 cites W2094740743 @default.
- W2045773985 cites W2096296961 @default.
- W2045773985 cites W2096434331 @default.
- W2045773985 cites W2099899478 @default.
- W2045773985 cites W2101338790 @default.
- W2045773985 cites W2102200294 @default.
- W2045773985 cites W2103917413 @default.
- W2045773985 cites W2106631130 @default.
- W2045773985 cites W2106730818 @default.
- W2045773985 cites W2112085782 @default.
- W2045773985 cites W2112264347 @default.
- W2045773985 cites W2113103309 @default.
- W2045773985 cites W2114261071 @default.
- W2045773985 cites W2114859361 @default.
- W2045773985 cites W2114878753 @default.
- W2045773985 cites W2115181164 @default.
- W2045773985 cites W2119336718 @default.
- W2045773985 cites W2121359567 @default.
- W2045773985 cites W2122439888 @default.
- W2045773985 cites W2126629855 @default.
- W2045773985 cites W2128555360 @default.
- W2045773985 cites W2128593976 @default.
- W2045773985 cites W2130391651 @default.
- W2045773985 cites W2133993745 @default.
- W2045773985 cites W2136634241 @default.
- W2045773985 cites W2138016026 @default.
- W2045773985 cites W2139863143 @default.
- W2045773985 cites W2140857141 @default.
- W2045773985 cites W2142374550 @default.
- W2045773985 cites W2142612221 @default.
- W2045773985 cites W2143226567 @default.
- W2045773985 cites W2145412955 @default.
- W2045773985 cites W2146299409 @default.
- W2045773985 cites W2148071606 @default.
- W2045773985 cites W2149777799 @default.
- W2045773985 cites W2150518523 @default.
- W2045773985 cites W2151845441 @default.
- W2045773985 cites W2152744284 @default.