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• W2045780917 abstract "ObjectiveTo evaluate the effect of induced blastocoele shrinkage before vitrification in a closed carrier device on clinical pregnancy and survival rates.DesignPrior to vitrification, blastocyst cavity was artificially reduced by laser pulse or not treated according to a randomized procedure. Clinical pregnancy rate and survival rate were assessed in 187 warming cycles.Materials and MethodsOn day 5 or 6 of early embryo development, full (3), expanded blastocyst (4AA, 4AB, 4BA, 4BB, 4CA or 4CB), partially hatched (5AA, 5AB, 5BA, 5BB, 5CA or 5CB) or fully hatched blastocysts (6) according to Gardner classification were cryopreserved. Clinical pregnancy rate was compared between blastocysts with induced shrinkage (n= 67 transfer cycles) and blastocysts without induced shrinkage used as control (n= 113 transfer cycles). Survival rate was also evaluated in each group (106 warmed blastocysts with artificial shrinkage vs 183 warmed blastocysts without artificial shrinkage). The mean number of replaced embryo was 1.5 and 1.46 in the shrinkage and the control group respectively. The mean (±SD) women age was 33.9 ±5 years for both groups.ResultsThe global clinical pregnancy rate per transfer after vitrification in our program was 44.4% (80/180). The pregnancy rate in the group with artificial reduction of the cavity was significantly higher (52.2% (35/67)) than in the control group (39.8% (45/113)) (ε=2.1, p=0.03). The overall survival rate after thawing was 94.1% (272/289). The survival rate in the artificial shrinkage group was higher compared with the control group but not significantly: 98.1% (104/106) and 91.8% (168/183) respectively (ε=0.80, p=0.42).ConclusionThis study reveals that artificial shrinkage of blastocoelic cavity by laser pulse before vitrification in a closed carrier device improves clinical pregnancy rate after warming. This is the first work assessing the benefit of induced collapse in a closed vitrification system. Further studies are needed to ensure the efficiency of this technique. ObjectiveTo evaluate the effect of induced blastocoele shrinkage before vitrification in a closed carrier device on clinical pregnancy and survival rates. To evaluate the effect of induced blastocoele shrinkage before vitrification in a closed carrier device on clinical pregnancy and survival rates. DesignPrior to vitrification, blastocyst cavity was artificially reduced by laser pulse or not treated according to a randomized procedure. Clinical pregnancy rate and survival rate were assessed in 187 warming cycles. Prior to vitrification, blastocyst cavity was artificially reduced by laser pulse or not treated according to a randomized procedure. Clinical pregnancy rate and survival rate were assessed in 187 warming cycles. Materials and MethodsOn day 5 or 6 of early embryo development, full (3), expanded blastocyst (4AA, 4AB, 4BA, 4BB, 4CA or 4CB), partially hatched (5AA, 5AB, 5BA, 5BB, 5CA or 5CB) or fully hatched blastocysts (6) according to Gardner classification were cryopreserved. Clinical pregnancy rate was compared between blastocysts with induced shrinkage (n= 67 transfer cycles) and blastocysts without induced shrinkage used as control (n= 113 transfer cycles). Survival rate was also evaluated in each group (106 warmed blastocysts with artificial shrinkage vs 183 warmed blastocysts without artificial shrinkage). The mean number of replaced embryo was 1.5 and 1.46 in the shrinkage and the control group respectively. The mean (±SD) women age was 33.9 ±5 years for both groups. On day 5 or 6 of early embryo development, full (3), expanded blastocyst (4AA, 4AB, 4BA, 4BB, 4CA or 4CB), partially hatched (5AA, 5AB, 5BA, 5BB, 5CA or 5CB) or fully hatched blastocysts (6) according to Gardner classification were cryopreserved. Clinical pregnancy rate was compared between blastocysts with induced shrinkage (n= 67 transfer cycles) and blastocysts without induced shrinkage used as control (n= 113 transfer cycles). Survival rate was also evaluated in each group (106 warmed blastocysts with artificial shrinkage vs 183 warmed blastocysts without artificial shrinkage). The mean number of replaced embryo was 1.5 and 1.46 in the shrinkage and the control group respectively. The mean (±SD) women age was 33.9 ±5 years for both groups. ResultsThe global clinical pregnancy rate per transfer after vitrification in our program was 44.4% (80/180). The pregnancy rate in the group with artificial reduction of the cavity was significantly higher (52.2% (35/67)) than in the control group (39.8% (45/113)) (ε=2.1, p=0.03). The overall survival rate after thawing was 94.1% (272/289). The survival rate in the artificial shrinkage group was higher compared with the control group but not significantly: 98.1% (104/106) and 91.8% (168/183) respectively (ε=0.80, p=0.42). The global clinical pregnancy rate per transfer after vitrification in our program was 44.4% (80/180). The pregnancy rate in the group with artificial reduction of the cavity was significantly higher (52.2% (35/67)) than in the control group (39.8% (45/113)) (ε=2.1, p=0.03). The overall survival rate after thawing was 94.1% (272/289). The survival rate in the artificial shrinkage group was higher compared with the control group but not significantly: 98.1% (104/106) and 91.8% (168/183) respectively (ε=0.80, p=0.42). ConclusionThis study reveals that artificial shrinkage of blastocoelic cavity by laser pulse before vitrification in a closed carrier device improves clinical pregnancy rate after warming. This is the first work assessing the benefit of induced collapse in a closed vitrification system. Further studies are needed to ensure the efficiency of this technique. This study reveals that artificial shrinkage of blastocoelic cavity by laser pulse before vitrification in a closed carrier device improves clinical pregnancy rate after warming. This is the first work assessing the benefit of induced collapse in a closed vitrification system. Further studies are needed to ensure the efficiency of this technique." @default.
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• W2045780917 date "2013-09-01" @default.
• W2045780917 modified "2023-10-14" @default.
• W2045780917 title "Effects of artificial shrinkage of human blastocysts prior to vitrification: a randomized controlled trial on 180 transfer cycles" @default.
• W2045780917 doi "https://doi.org/10.1016/j.fertnstert.2013.07.1830" @default.
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