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• W2045781131 abstract "A simple, high-throughput fluorescent assay was developed to measure the inhibition of membrane-bound CD45 from Jurkat cells. This assay is based on the fact that approximately 64% of PTP activity from Jurkat cell membrane is contributed by CD45. This has been proven by comparing the activity in membrane protein from wild-type Jurkat cells and CD45-negative mutant cells, and also by measuring the residual activity after depleting CD45 from Jurkat cell membrane. We have demonstrated that fluorescein diphosphate can be used as a substrate to monitor CD45 activity from Jurkat cell membrane, which allows us to easily follow CD45 activity in both fluorescent and absorbance modes in a 96-well format. Some common protein tyrosine phosphatase inhibitors have been evaluated with this assay." @default.
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• W2045781131 date "2000-10-01" @default.
• W2045781131 modified "2023-09-28" @default.
• W2045781131 title "Development of a Method for Evaluating Protein Tyrosine Phosphatase CD45 Inhibitors Using Jurkat Cell Membrane" @default.
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• W2045781131 doi "https://doi.org/10.1006/abio.2000.4732" @default.
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