FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2045783522> ?p ?o ?g. }

• W2045783522 endingPage "41668" @default.
• W2045783522 startingPage "41656" @default.
• W2045783522 abstract "The molecular architecture and composition of the outer membrane (OM) of Treponema pallidum (Tp), the noncultivable agent of venereal syphilis, differ considerably from those of typical Gram-negative bacteria. Several years ago we described TP0453, the only lipoprotein associated with the inner leaflet of the Tp OM. Whereas polypeptides of other treponemal lipoproteins are hydrophilic, non-lipidated TP0453 can integrate into membranes, a property attributed to its multiple amphipathic helices (AHs). Furthermore, membrane integration of the TP0453 polypeptide was found to increase membrane permeability, suggesting the molecule functions in a porin-like manner. To better understand the mechanism of membrane integration of TP0453 and its physiological role in Tp OM biogenesis, we solved its crystal structure and used mutagenesis to identify membrane insertion elements. The crystal structure of TP0453 consists of an α/β/α-fold and includes five stably folded AHs. In high concentrations of detergent, TP0453 transitions from a closed to open conformation by lateral movement of two groups of AHs, exposing a large hydrophobic cavity. Triton X-114 phase partitioning, liposome floatation assay, and bis-1-anilino-8-naphthalenesulfonate binding revealed that two adjacent AHs are critical for membrane sensing/integration. Using terbium-dipicolinic acid complex-loaded large unilamellar vesicles, we found that TP0453 increased efflux of fluorophore only at acidic pH. Gel filtration and cross-linking experiments demonstrated that one AH critical for membrane sensing/insertion also forms a dimeric interface. Based on structural dynamics and comparison with Mycobacterium tuberculosis lipoproteins LprG and LppX, we propose that TP0453 functions as a carrier of lipids, glycolipids, and/or derivatives during OM biogenesis." @default.
• W2045783522 created "2016-06-24" @default.
• W2045783522 creator A5002622514 @default.
• W2045783522 creator A5002868089 @default.
• W2045783522 creator A5008416064 @default.
• W2045783522 creator A5013263926 @default.
• W2045783522 creator A5031909097 @default.
• W2045783522 creator A5048445348 @default.
• W2045783522 creator A5056897795 @default.
• W2045783522 creator A5064035517 @default.
• W2045783522 creator A5066994898 @default.
• W2045783522 creator A5073503247 @default.
• W2045783522 creator A5086390634 @default.
• W2045783522 creator A5089998022 @default.
• W2045783522 date "2011-12-01" @default.
• W2045783522 modified "2023-09-28" @default.
• W2045783522 title "The Transition from Closed to Open Conformation of Treponema pallidum Outer Membrane-associated Lipoprotein TP0453 Involves Membrane Sensing and Integration by Two Amphipathic Helices" @default.
• W2045783522 cites W1504177403 @default.
• W2045783522 cites W1510483750 @default.
• W2045783522 cites W1533024834 @default.
• W2045783522 cites W1539796472 @default.
• W2045783522 cites W1582810658 @default.
• W2045783522 cites W159094262 @default.
• W2045783522 cites W1591680814 @default.
• W2045783522 cites W1626320416 @default.
• W2045783522 cites W1658195499 @default.
• W2045783522 cites W1956214045 @default.
• W2045783522 cites W1958594228 @default.
• W2045783522 cites W1966272186 @default.
• W2045783522 cites W1966893537 @default.
• W2045783522 cites W1967946321 @default.
• W2045783522 cites W1972640555 @default.
• W2045783522 cites W1983041459 @default.
• W2045783522 cites W1995584045 @default.
• W2045783522 cites W1995954548 @default.
• W2045783522 cites W2000992203 @default.
• W2045783522 cites W2007142983 @default.
• W2045783522 cites W2013067557 @default.
• W2045783522 cites W2017727500 @default.
• W2045783522 cites W2031977066 @default.
• W2045783522 cites W2035163170 @default.
• W2045783522 cites W2038840577 @default.
• W2045783522 cites W2047143958 @default.
• W2045783522 cites W2054572399 @default.
• W2045783522 cites W2060009917 @default.
• W2045783522 cites W2061681936 @default.
• W2045783522 cites W2068119233 @default.
• W2045783522 cites W2068972207 @default.
• W2045783522 cites W2070839892 @default.
• W2045783522 cites W2071700210 @default.
• W2045783522 cites W2075930146 @default.
• W2045783522 cites W2077244176 @default.
• W2045783522 cites W2082047232 @default.
• W2045783522 cites W2085101416 @default.
• W2045783522 cites W2098594736 @default.
• W2045783522 cites W2100641815 @default.
• W2045783522 cites W2104976332 @default.
• W2045783522 cites W2108921801 @default.
• W2045783522 cites W2112200653 @default.
• W2045783522 cites W2119069290 @default.
• W2045783522 cites W2127594598 @default.
• W2045783522 cites W2132065459 @default.
• W2045783522 cites W2137450588 @default.
• W2045783522 cites W2140260269 @default.
• W2045783522 cites W2144081223 @default.
• W2045783522 cites W2147220791 @default.
• W2045783522 cites W2160124608 @default.
• W2045783522 cites W2163341755 @default.
• W2045783522 cites W2163846478 @default.
• W2045783522 cites W2168995883 @default.
• W2045783522 cites W2201666950 @default.
• W2045783522 cites W2224089154 @default.
• W2045783522 cites W2316159845 @default.
• W2045783522 doi "https://doi.org/10.1074/jbc.m111.305284" @default.
• W2045783522 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3308875" @default.
• W2045783522 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21965687" @default.
• W2045783522 hasPublicationYear "2011" @default.
• W2045783522 type Work @default.
• W2045783522 sameAs 2045783522 @default.
• W2045783522 citedByCount "23" @default.
• W2045783522 countsByYear W20457835222012 @default.
• W2045783522 countsByYear W20457835222013 @default.
• W2045783522 countsByYear W20457835222014 @default.
• W2045783522 countsByYear W20457835222015 @default.
• W2045783522 countsByYear W20457835222016 @default.
• W2045783522 countsByYear W20457835222017 @default.
• W2045783522 countsByYear W20457835222018 @default.
• W2045783522 countsByYear W20457835222019 @default.
• W2045783522 countsByYear W20457835222020 @default.
• W2045783522 countsByYear W20457835222022 @default.
• W2045783522 countsByYear W20457835222023 @default.
• W2045783522 crossrefType "journal-article" @default.
• W2045783522 hasAuthorship W2045783522A5002622514 @default.
• W2045783522 hasAuthorship W2045783522A5002868089 @default.
• W2045783522 hasAuthorship W2045783522A5008416064 @default.
• W2045783522 hasAuthorship W2045783522A5013263926 @default.
• W2045783522 hasAuthorship W2045783522A5031909097 @default.
• W2045783522 hasAuthorship W2045783522A5048445348 @default.

• next