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- W2045784782 abstract "In the course of pulse-label studies on the axonal transport of the small, basic, actin-binding proteins – actin depolymerizing factor, cofilin and profilin – in chicken motor neurones, we observed a heavily labelled protein of Mr 18 kDa and pI 8.2 on fluorographs of two-dimensional polyacrylamide gels. On the basis of its Mr, pI and amino acid composition, we tentatively identified it by database searching as cyclophilin A and subsequently confirmed its identity by immunostaining. Like actin and its associated proteins, cyclophilin A was transported in slow component b of axonal transport, but unlike these proteins, cyclophilin A did not copurify with actin on DNase I. It was not found amongst labelled proteins transported by fast axonal transport. Immunostaining of chicken dorsal root ganglion cells revealed that it accumulated in neurites at points of branching, varicosities and growth cones. Our results raise the possibility that cyclophilin A is important in maintaining the native folding of actin and associated proteins during transit in axons and assembly in growth cones." @default.
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- W2045784782 date "1997-10-01" @default.
- W2045784782 modified "2023-09-29" @default.
- W2045784782 title "Axonal transport and distribution of cyclophilin A in chicken neurones" @default.
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- W2045784782 doi "https://doi.org/10.1016/s0006-8993(97)00766-x" @default.
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