Matches in SemOpenAlex for { <https://semopenalex.org/work/W2045787584> ?p ?o ?g. }
- W2045787584 endingPage "260" @default.
- W2045787584 startingPage "252" @default.
- W2045787584 abstract "The photoaffinity analog of ATP, 8-azidoATP, labels T7 RNA polymerase. Photoincorporation exhibits saturation behavior and is protected against by the substrate ATP. 8-AzidoATP is a competitive inhibitor of ATP incorporation with Ki ∼ 40 μM. The photolabeled T7 RNA polymerase, following cyanogen bromide digestion, was analyzed by phenylboronate agarose column chromatography followed by reverse-phase high pressure liquid chromatography. Sequencing of the peptides labeled with radioactive photoprobe allowed the identification of three peptides, P314-M362 (I), L550-M666 (II), and F751-M861 (III). These peptides are in the proximity of the photoprobe 8-azidoATP and, therefore, expected to contain functionally significant residues and define an active site domain. These peptides (I and II) contain residues previously implicated in T7 RNA polymerase activity or show homology to active site regions of the Klenow fragment of DNA polymerase I (II and III)." @default.
- W2045787584 created "2016-06-24" @default.
- W2045787584 creator A5003547895 @default.
- W2045787584 creator A5070377413 @default.
- W2045787584 creator A5070741598 @default.
- W2045787584 date "1992-06-01" @default.
- W2045787584 modified "2023-10-14" @default.
- W2045787584 title "Mapping of the active site of T7 RNA polymerase with 8-azidoATP" @default.
- W2045787584 cites W1232330100 @default.
- W2045787584 cites W1486403021 @default.
- W2045787584 cites W1487026581 @default.
- W2045787584 cites W1492300677 @default.
- W2045787584 cites W1494066298 @default.
- W2045787584 cites W1501105062 @default.
- W2045787584 cites W1524470368 @default.
- W2045787584 cites W1555625531 @default.
- W2045787584 cites W1576801374 @default.
- W2045787584 cites W1591184066 @default.
- W2045787584 cites W1673579390 @default.
- W2045787584 cites W1805448417 @default.
- W2045787584 cites W1932052686 @default.
- W2045787584 cites W1966890039 @default.
- W2045787584 cites W1970009965 @default.
- W2045787584 cites W1974306517 @default.
- W2045787584 cites W1975085160 @default.
- W2045787584 cites W1977661451 @default.
- W2045787584 cites W1979511146 @default.
- W2045787584 cites W1997398493 @default.
- W2045787584 cites W2002692958 @default.
- W2045787584 cites W2002841189 @default.
- W2045787584 cites W2013997494 @default.
- W2045787584 cites W2026237973 @default.
- W2045787584 cites W2033063625 @default.
- W2045787584 cites W2035650458 @default.
- W2045787584 cites W2043916998 @default.
- W2045787584 cites W2044831294 @default.
- W2045787584 cites W2046643787 @default.
- W2045787584 cites W2046780866 @default.
- W2045787584 cites W2057263492 @default.
- W2045787584 cites W2059367441 @default.
- W2045787584 cites W2063115551 @default.
- W2045787584 cites W2075583438 @default.
- W2045787584 cites W2092037376 @default.
- W2045787584 cites W2095450147 @default.
- W2045787584 cites W2098514662 @default.
- W2045787584 cites W2100837269 @default.
- W2045787584 cites W2103947911 @default.
- W2045787584 cites W2126719129 @default.
- W2045787584 cites W2166172853 @default.
- W2045787584 cites W2208102268 @default.
- W2045787584 cites W2328748793 @default.
- W2045787584 cites W986536428 @default.
- W2045787584 cites W989164380 @default.
- W2045787584 doi "https://doi.org/10.1016/0167-4838(92)90154-6" @default.
- W2045787584 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1627602" @default.
- W2045787584 hasPublicationYear "1992" @default.
- W2045787584 type Work @default.
- W2045787584 sameAs 2045787584 @default.
- W2045787584 citedByCount "14" @default.
- W2045787584 countsByYear W20457875842016 @default.
- W2045787584 crossrefType "journal-article" @default.
- W2045787584 hasAuthorship W2045787584A5003547895 @default.
- W2045787584 hasAuthorship W2045787584A5070377413 @default.
- W2045787584 hasAuthorship W2045787584A5070741598 @default.
- W2045787584 hasConcept C104317684 @default.
- W2045787584 hasConcept C107824862 @default.
- W2045787584 hasConcept C153911025 @default.
- W2045787584 hasConcept C156719811 @default.
- W2045787584 hasConcept C164965036 @default.
- W2045787584 hasConcept C167625842 @default.
- W2045787584 hasConcept C185592680 @default.
- W2045787584 hasConcept C2776441376 @default.
- W2045787584 hasConcept C2777093645 @default.
- W2045787584 hasConcept C2777501222 @default.
- W2045787584 hasConcept C2778581200 @default.
- W2045787584 hasConcept C2780048736 @default.
- W2045787584 hasConcept C54415193 @default.
- W2045787584 hasConcept C547475151 @default.
- W2045787584 hasConcept C552990157 @default.
- W2045787584 hasConcept C55493867 @default.
- W2045787584 hasConcept C67705224 @default.
- W2045787584 hasConcept C82381507 @default.
- W2045787584 hasConcept C86803240 @default.
- W2045787584 hasConceptScore W2045787584C104317684 @default.
- W2045787584 hasConceptScore W2045787584C107824862 @default.
- W2045787584 hasConceptScore W2045787584C153911025 @default.
- W2045787584 hasConceptScore W2045787584C156719811 @default.
- W2045787584 hasConceptScore W2045787584C164965036 @default.
- W2045787584 hasConceptScore W2045787584C167625842 @default.
- W2045787584 hasConceptScore W2045787584C185592680 @default.
- W2045787584 hasConceptScore W2045787584C2776441376 @default.
- W2045787584 hasConceptScore W2045787584C2777093645 @default.
- W2045787584 hasConceptScore W2045787584C2777501222 @default.
- W2045787584 hasConceptScore W2045787584C2778581200 @default.
- W2045787584 hasConceptScore W2045787584C2780048736 @default.
- W2045787584 hasConceptScore W2045787584C54415193 @default.
- W2045787584 hasConceptScore W2045787584C547475151 @default.
- W2045787584 hasConceptScore W2045787584C552990157 @default.