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- W2045796800 abstract "Abstract The microtubule plus-end tracking proteins (+TIPs) END BINDING1b (EB1b) and SPIRAL1 (SPR1) are required for normal cell expansion and organ growth. EB proteins are viewed as central regulators of +TIPs and cell polarity in animals; SPR1 homologs are specific to plants. To explore if EB1b and SPR1 fundamentally function together, we combined genetic, biochemical, and cell imaging approaches in Arabidopsis thaliana. We found that eb1b-2 spr1-6 double mutant roots exhibit substantially more severe polar expansion defects than either single mutant, undergoing right-looping growth and severe axial twisting instead of waving on tilted hard-agar surfaces. Protein interaction assays revealed that EB1b and SPR1 bind each other and tubulin heterodimers, which is suggestive of a microtubule loading mechanism. EB1b and SPR1 show antagonistic association with microtubules in vitro. Surprisingly, our combined analyses revealed that SPR1 can load onto microtubules and function independently of EB1 proteins, setting SPR1 apart from most studied +TIPs in animals and fungi. Moreover, we found that the severity of defects in microtubule dynamics in spr1 eb1b mutant hypocotyl cells correlated well with the severity of growth defects. These data indicate that SPR1 and EB1b have complex interactions as they load onto microtubule plus ends and direct polar cell expansion and organ growth in response to directional cues." @default.
- W2045796800 created "2016-06-24" @default.
- W2045796800 creator A5009883772 @default.
- W2045796800 creator A5017428056 @default.
- W2045796800 creator A5030286735 @default.
- W2045796800 creator A5032212672 @default.
- W2045796800 creator A5045629380 @default.
- W2045796800 creator A5055642199 @default.
- W2045796800 creator A5061101348 @default.
- W2045796800 creator A5065012016 @default.
- W2045796800 creator A5080907609 @default.
- W2045796800 date "2014-11-01" @default.
- W2045796800 modified "2023-09-30" @default.
- W2045796800 title "The Microtubule Plus-End Tracking Proteins SPR1 and EB1b Interact to Maintain Polar Cell Elongation and Directional Organ Growth in <i>Arabidopsis</i> " @default.
- W2045796800 cites W12220879 @default.
- W2045796800 cites W1518891633 @default.
- W2045796800 cites W1583509996 @default.
- W2045796800 cites W1606831709 @default.
- W2045796800 cites W1908201726 @default.
- W2045796800 cites W1977919339 @default.
- W2045796800 cites W1977926121 @default.
- W2045796800 cites W1980688357 @default.
- W2045796800 cites W1981919641 @default.
- W2045796800 cites W1982743797 @default.
- W2045796800 cites W1985501881 @default.
- W2045796800 cites W1987948344 @default.
- W2045796800 cites W1988129569 @default.
- W2045796800 cites W1988681627 @default.
- W2045796800 cites W1989307968 @default.
- W2045796800 cites W1990022480 @default.
- W2045796800 cites W1995462733 @default.
- W2045796800 cites W1995834512 @default.
- W2045796800 cites W1996535162 @default.
- W2045796800 cites W1997456815 @default.
- W2045796800 cites W2003293851 @default.
- W2045796800 cites W2008016162 @default.
- W2045796800 cites W2008921607 @default.
- W2045796800 cites W2009233191 @default.
- W2045796800 cites W2013447580 @default.
- W2045796800 cites W2015736998 @default.
- W2045796800 cites W2016121442 @default.
- W2045796800 cites W2018051595 @default.
- W2045796800 cites W2020042592 @default.
- W2045796800 cites W2020906481 @default.
- W2045796800 cites W2023181098 @default.
- W2045796800 cites W2027382153 @default.
- W2045796800 cites W2028377541 @default.
- W2045796800 cites W2035018334 @default.
- W2045796800 cites W2035390791 @default.
- W2045796800 cites W2038594751 @default.
- W2045796800 cites W2044780741 @default.
- W2045796800 cites W2055513381 @default.
- W2045796800 cites W2058411072 @default.
- W2045796800 cites W2058557578 @default.
- W2045796800 cites W2058761402 @default.
- W2045796800 cites W2059588721 @default.
- W2045796800 cites W2060259273 @default.
- W2045796800 cites W2060437620 @default.
- W2045796800 cites W2073138983 @default.
- W2045796800 cites W2077396975 @default.
- W2045796800 cites W2079119354 @default.
- W2045796800 cites W2082490153 @default.
- W2045796800 cites W2087160222 @default.
- W2045796800 cites W2093155812 @default.
- W2045796800 cites W2093884383 @default.
- W2045796800 cites W2095658523 @default.
- W2045796800 cites W2096217862 @default.
- W2045796800 cites W2096682961 @default.
- W2045796800 cites W2098747934 @default.
- W2045796800 cites W2109335417 @default.
- W2045796800 cites W2115561264 @default.
- W2045796800 cites W2116122104 @default.
- W2045796800 cites W2117798180 @default.
- W2045796800 cites W2119749663 @default.
- W2045796800 cites W2120389464 @default.
- W2045796800 cites W2121927846 @default.
- W2045796800 cites W2124534459 @default.
- W2045796800 cites W2125861134 @default.
- W2045796800 cites W2129158700 @default.
- W2045796800 cites W2130589938 @default.
- W2045796800 cites W2133090102 @default.
- W2045796800 cites W2133860019 @default.
- W2045796800 cites W2134118659 @default.
- W2045796800 cites W2137036197 @default.
- W2045796800 cites W2140995916 @default.
- W2045796800 cites W2141527229 @default.
- W2045796800 cites W2141734192 @default.
- W2045796800 cites W2144549134 @default.
- W2045796800 cites W2145669960 @default.
- W2045796800 cites W2146320461 @default.
- W2045796800 cites W2150402884 @default.
- W2045796800 cites W2151757423 @default.
- W2045796800 cites W2155289420 @default.
- W2045796800 cites W2157587925 @default.
- W2045796800 cites W2158395856 @default.
- W2045796800 cites W2159135766 @default.
- W2045796800 cites W2164825799 @default.
- W2045796800 cites W2164962038 @default.