Matches in SemOpenAlex for { <https://semopenalex.org/work/W2045796821> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2045796821 endingPage "592" @default.
- W2045796821 startingPage "585" @default.
- W2045796821 abstract "The enantioselectivity of lipase from Burkhorderia cepacia KWI-56 has been inverted using a novel in vitro technique for construction and screening of a protein library by single-molecule DNA amplification by PCR followed by in vitro coupled transcription/translation system termed single-molecule-PCR-linked in vitro expression (SIMPLEX). Four amino acid residues (L17, F119, L167, and L266) in the hydrophobic substrate-binding pocket of the lipase were selected for mutation based on a structural model of a substrate-enzyme complex, and a combinatorial mutation library was constructed by SIMPLEX and screened for (R) and (S)-configurations of p-nitrophenyl 3-phenylbutyrate. Some combinations of amino acid substitutions in the four positions of the lipase were found as effective for changing the enantiopreference from the (S)-form substrate to the (R)-form. Two variants were expressed in the original host cells and purified to homogeneity, showing completely reversed enantioselectivity for the (R)-form of ethyl 3-phenylbutyrate (selectivity factor E(R)=38 or 33), whereas the wild-type lipase was (S)-selective (selectivity factor E(S)=33). Thus the semi-rational and semi-random combinatorial design of a mutant library followed by a high-throughput screening based on their enzymatic activity should be a powerful tool to engineer the enantioselectivity of enzymes." @default.
- W2045796821 created "2016-06-24" @default.
- W2045796821 creator A5032616275 @default.
- W2045796821 creator A5070778902 @default.
- W2045796821 creator A5080277117 @default.
- W2045796821 creator A5081978092 @default.
- W2045796821 date "2003-08-01" @default.
- W2045796821 modified "2023-10-17" @default.
- W2045796821 title "Inverting Enantioselectivity of Burkholderia cepacia KWI-56 Lipase by Combinatorial Mutation and High-throughput Screening Using Single-molecule PCR and In Vitro Expression" @default.
- W2045796821 cites W1970441849 @default.
- W2045796821 cites W1974274392 @default.
- W2045796821 cites W1980563461 @default.
- W2045796821 cites W1989581832 @default.
- W2045796821 cites W1998008547 @default.
- W2045796821 cites W2004315636 @default.
- W2045796821 cites W2055709527 @default.
- W2045796821 cites W2058237375 @default.
- W2045796821 cites W2062281244 @default.
- W2045796821 cites W2077180437 @default.
- W2045796821 cites W2078303301 @default.
- W2045796821 cites W2081291309 @default.
- W2045796821 cites W2085814338 @default.
- W2045796821 cites W2105795692 @default.
- W2045796821 cites W2124265937 @default.
- W2045796821 cites W2137473984 @default.
- W2045796821 cites W2156972574 @default.
- W2045796821 cites W2158495980 @default.
- W2045796821 cites W2161090571 @default.
- W2045796821 cites W4231715140 @default.
- W2045796821 doi "https://doi.org/10.1016/s0022-2836(03)00782-4" @default.
- W2045796821 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12899830" @default.
- W2045796821 hasPublicationYear "2003" @default.
- W2045796821 type Work @default.
- W2045796821 sameAs 2045796821 @default.
- W2045796821 citedByCount "100" @default.
- W2045796821 countsByYear W20457968212012 @default.
- W2045796821 countsByYear W20457968212013 @default.
- W2045796821 countsByYear W20457968212014 @default.
- W2045796821 countsByYear W20457968212016 @default.
- W2045796821 countsByYear W20457968212017 @default.
- W2045796821 countsByYear W20457968212021 @default.
- W2045796821 countsByYear W20457968212022 @default.
- W2045796821 crossrefType "journal-article" @default.
- W2045796821 hasAuthorship W2045796821A5032616275 @default.
- W2045796821 hasAuthorship W2045796821A5070778902 @default.
- W2045796821 hasAuthorship W2045796821A5080277117 @default.
- W2045796821 hasAuthorship W2045796821A5081978092 @default.
- W2045796821 hasConcept C104317684 @default.
- W2045796821 hasConcept C143065580 @default.
- W2045796821 hasConcept C181199279 @default.
- W2045796821 hasConcept C185592680 @default.
- W2045796821 hasConcept C202751555 @default.
- W2045796821 hasConcept C2779697368 @default.
- W2045796821 hasConcept C2780120296 @default.
- W2045796821 hasConcept C2780855054 @default.
- W2045796821 hasConcept C51323132 @default.
- W2045796821 hasConcept C523546767 @default.
- W2045796821 hasConcept C54355233 @default.
- W2045796821 hasConcept C55493867 @default.
- W2045796821 hasConcept C71240020 @default.
- W2045796821 hasConcept C86803240 @default.
- W2045796821 hasConceptScore W2045796821C104317684 @default.
- W2045796821 hasConceptScore W2045796821C143065580 @default.
- W2045796821 hasConceptScore W2045796821C181199279 @default.
- W2045796821 hasConceptScore W2045796821C185592680 @default.
- W2045796821 hasConceptScore W2045796821C202751555 @default.
- W2045796821 hasConceptScore W2045796821C2779697368 @default.
- W2045796821 hasConceptScore W2045796821C2780120296 @default.
- W2045796821 hasConceptScore W2045796821C2780855054 @default.
- W2045796821 hasConceptScore W2045796821C51323132 @default.
- W2045796821 hasConceptScore W2045796821C523546767 @default.
- W2045796821 hasConceptScore W2045796821C54355233 @default.
- W2045796821 hasConceptScore W2045796821C55493867 @default.
- W2045796821 hasConceptScore W2045796821C71240020 @default.
- W2045796821 hasConceptScore W2045796821C86803240 @default.
- W2045796821 hasIssue "3" @default.
- W2045796821 hasLocation W20457968211 @default.
- W2045796821 hasLocation W20457968212 @default.
- W2045796821 hasOpenAccess W2045796821 @default.
- W2045796821 hasPrimaryLocation W20457968211 @default.
- W2045796821 hasRelatedWork W1972136774 @default.
- W2045796821 hasRelatedWork W2001076025 @default.
- W2045796821 hasRelatedWork W2002230344 @default.
- W2045796821 hasRelatedWork W2027018170 @default.
- W2045796821 hasRelatedWork W2072967036 @default.
- W2045796821 hasRelatedWork W2094836748 @default.
- W2045796821 hasRelatedWork W2155835698 @default.
- W2045796821 hasRelatedWork W2359434416 @default.
- W2045796821 hasRelatedWork W2780993610 @default.
- W2045796821 hasRelatedWork W1899174466 @default.
- W2045796821 hasVolume "331" @default.
- W2045796821 isParatext "false" @default.
- W2045796821 isRetracted "false" @default.
- W2045796821 magId "2045796821" @default.
- W2045796821 workType "article" @default.