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- W2045797847 abstract "A Polyomavirus mutant with a 504 base pair deletion downstream of the polyadenylation signal for the early genes was constructed in vitro. This mutant showed a reduced synthesis of viral DNA. In cotransfection experiments, this defect was complemented by the presence of a wild-type genome. To define the sequences involved in the determination of this phenotype, a set of viral mutants was constructed. The properties of these mutants suggested that the deletion of a short DNA segment located 35 base pairs downstream of the early polyadenylation site affected the stability of early mRNA. The boundaries of the deletion were within the late coding sequences. However, the truncated form of the major capsid protein VP1 expressed by the mutant, did not influence the formation of early mRNA and the synthesis of viral DNA." @default.
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- W2045797847 date "1991-07-01" @default.
- W2045797847 modified "2023-09-25" @default.
- W2045797847 title "Reduced Polyomavirus DNA replication as a consequence of a late-region deletion that results in early mRNA instability" @default.
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- W2045797847 doi "https://doi.org/10.1016/0168-1702(91)90106-6" @default.
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