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- W2045799499 abstract "Human plasma samples of 1 ml were processed according to three different procedures prior to Radioimmunoassay (RIA) of Prostaglandin F2α (PGF2α). Serial dilutions of ethyl acetate extracts as such, or combined with either silicic acid of Sephadex G-25 chromatography were assessed for linearity, homogeneity and parallelism with the corresponding standard dose response line. For plasma extracts used as such, non-parallelism is observed. Subsequent chromatography on silicic acid of such extracts gave only a limited linear and parallel portion upon serial dilution. However, purification of the extracts by gelfiltration on Sephadex G-25 results in linear and parallel lines over the full range of the standard dose response line (B/Bo 0.9-0.2). Upon comparison of separation by polyethylene glycol (PEG) and Dextran coated charcoal (DCC) in these systems, PEG proved to give the best results. It was found that in the Sephadex G-25 procedure, separation by PEG is essential. The method of gelfiltration on Sephadex G-25 is simple and reliable. Intra- and inter-assay coefficients of variation are 6% and 12%, respectively. Accuracy, as measured by recovery of added known amounts of PGF2α is 97.6%." @default.
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- W2045799499 date "1978-05-01" @default.
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- W2045799499 title "Radioimmunoassays for prostaglandins. I. Technical validation of prostaglandin F2α measurements in human plasma using Sephadex G-25 gelfiltration" @default.
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- W2045799499 doi "https://doi.org/10.1016/0090-6980(78)90150-8" @default.
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