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- W2045843679 abstract "An assay procedure for thyroid hormone receptor activity which used nitrocellulose membrane filters was developed. Receptor proteins, extracted from washed rat liver nuclei with a 0.4 m NaCl solution, were incubated with 125I-labeled thyroid hormone (T3), and filtered on the cellulose ester membranes under suction at 2°C. The filters were subsequently washed with cold buffer and counted for 125I radioactivity. The method allowed an accurate estimation of the receptor activity, satisfying a linear relationship between the activity and the receptor protein concentrations. The usefulness of this filter-binding method became evident when it was compared with the conventional procedure that employs Sephadex G-25 columns. For practical application to routine assays, various filtration conditions were examined, and a standard procedure was established. Using this technique, the isolated receptors were determined to possess an apparent Kd of 1.38 × 10−10m and a pH optimum of T3 binding at 8.2–8.4." @default.
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- W2045843679 date "1983-10-01" @default.
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- W2045843679 title "Filter-binding assay procedure for thyroid hormone receptors" @default.
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- W2045843679 doi "https://doi.org/10.1016/0003-2697(83)90280-4" @default.
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