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• W2045878597 abstract "The distribution and morphology of the endoplasmic reticulum (ER) in mammalian cells depend on both dynamic and static interactions of ER membrane proteins with microtubules (MTs). Cytoskeleton-linking membrane protein (CLIMP)-63 is exclusively localized in sheet-like ER membranes, typical structures of the rough ER, and plays a pivotal role in the static interaction with MTs. Our previous study showed that the 42-kDa ER-residing form of syntaxin 5 (Syn5L) regulates ER structure through the interactions with both CLIMP-63 and MTs. Here, we extend our previous study and show that the valosin-containing protein/p97-interacting membrane protein (VIMP)/SelS is also a member of the family of proteins that shape the ER by interacting with MTs. Depletion of VIMP causes the spreading of the ER to the cell periphery and affects an MT-dependent process on the ER. Although VIMP can interact with CLIMP-63 and Syn5L, it does not interact with MT-binding ER proteins (such as Reep1) that shape the tubular smooth ER, suggesting that different sets of MT-binding ER proteins are used to organize different ER subdomains. The distribution and morphology of the endoplasmic reticulum (ER) in mammalian cells depend on both dynamic and static interactions of ER membrane proteins with microtubules (MTs). Cytoskeleton-linking membrane protein (CLIMP)-63 is exclusively localized in sheet-like ER membranes, typical structures of the rough ER, and plays a pivotal role in the static interaction with MTs. Our previous study showed that the 42-kDa ER-residing form of syntaxin 5 (Syn5L) regulates ER structure through the interactions with both CLIMP-63 and MTs. Here, we extend our previous study and show that the valosin-containing protein/p97-interacting membrane protein (VIMP)/SelS is also a member of the family of proteins that shape the ER by interacting with MTs. Depletion of VIMP causes the spreading of the ER to the cell periphery and affects an MT-dependent process on the ER. Although VIMP can interact with CLIMP-63 and Syn5L, it does not interact with MT-binding ER proteins (such as Reep1) that shape the tubular smooth ER, suggesting that different sets of MT-binding ER proteins are used to organize different ER subdomains. The endoplasmic reticulum (ER) 3The abbreviations used are: ERendoplasmic reticulumMTmicrotubuleSynsyntaxinCLIMPcytoskeleton-linking membrane proteinRTNreticulonATLatlastinVCPvalosin-containing proteinVIMPVCP/p97-interacting membrane proteinBFAbrefeldin AmRFPmonomeric red fluorescent proteinPFAparaformaldehydeVSVGvesicular stomatitis virus-encoded glycoprotein. is a continuous membrane system but consists of a variety of subdomains that are responsible for executing diverse functions of the ER, such as protein synthesis and export, lipid synthesis, calcium homeostasis, and protein degradation (which is known as ER-associated degradation) (1.Lynes E.M. Simmen T. Urban planning of the endoplasmic reticulum (ER): how diverse mechanisms segregate the many functions of the ER.Biochim. Biophys. Acta. 2011; 1813: 1893-1905Crossref PubMed Scopus (101) Google Scholar). Morphologically, the ER structure can be classified into two general groups: tubular and sheet-like structures that represent typical smooth and rough ER membrane structures, respectively (2.Shibata Y. Hu J. Kozlov M.M. Rapoport T.A. Mechanisms shaping the membranes of cellular organelles.Annu. Rev. Cell Dev. Biol. 2009; 25: 329-354Crossref PubMed Scopus (315) Google Scholar, 3.Friedman J.R. Voeltz G.K. The ER in 3D: a multifunctional dynamic membrane network.Trends Cell Biol. 2011; 21: 709-717Abstract Full Text Full Text PDF PubMed Scopus (212) Google Scholar, 4.Chen S. Novick P. Ferro-Novick S. ER structure and function.Curr. Opin. Cell Biol. 2013; 25: 428-433Crossref PubMed Scopus (121) Google Scholar, 5.Goyal U. Blackstone C. Untangling the web: mechanisms underlying ER network formation.Biochim. Biophys. Acta. 2013; 1833: 2492-2498Crossref PubMed Scopus (112) Google Scholar). endoplasmic reticulum microtubule syntaxin cytoskeleton-linking membrane protein reticulon atlastin valosin-containing protein VCP/p97-interacting membrane protein brefeldin A monomeric red fluorescent protein paraformaldehyde vesicular stomatitis virus-encoded glycoprotein. Microtubules (MTs) play important roles in the organization and dynamics of ER membranes in mammalian cells via their dynamic and static interactions with ER-residing proteins (6.Vedrenne C. Hauri H.-P. Morphogenesis of the endoplasmic reticulum: beyond active membrane expansion.Traffic. 2006; 7: 639-646Crossref PubMed Scopus (129) Google Scholar). For example, ER membranes bind to the growing tips of MTs through an ER membrane protein, STIM1, and an MT plus end-binding protein, EB1 (7.Grigoriev I. Gouveia S.M. van der Vaart B. Demmers J. Smyth J.T. Honnappa S. Splinter D. Steinmetz M.O. Putney Jr., J.W. Hoogenraad C.C. Akhmanova A. STIM1 is a MT-plus-end-tracking protein involved in remodeling of the ER.Curr. Biol. 2008; 18: 177-182Abstract Full Text Full Text PDF PubMed Scopus (332) Google Scholar, 8.Waterman-Storer C.M. Salmon E.D. Endoplasmic reticulum membrane tubules are distributed by microtubules in living cells using three distinct mechanisms.Curr. Biol. 1998; 8: 798-806Abstract Full Text Full Text PDF PubMed Google Scholar). ER tubules bind to the shaft of an existing MT and slide along MTs (8.Waterman-Storer C.M. Salmon E.D. Endoplasmic reticulum membrane tubules are distributed by microtubules in living cells using three distinct mechanisms.Curr. Biol. 1998; 8: 798-806Abstract Full Text Full Text PDF PubMed Google Scholar, 9.Lee C. Ferguson M. Chen L.B. Construction of the endoplasmic reticulum.J. Cell Biol. 1989; 109: 2045-2055Crossref PubMed Scopus (119) Google Scholar), in particular, on curved acetylated MTs (10.Friedman J.R. Webster B.M. Mastronarde D.N. Verhey K.J. Voeltz G.K. ER sliding dynamics and ER-mitochondrial contacts occur on acetylated microtubules.J. Cell Biol. 2010; 190: 363-375Crossref PubMed Scopus (271) Google Scholar). Sliding is driven by MT motors, kinesin and cytoplasmic dynein (11.Woźniak M.J. Allan V.J. Cargo selection by specific kinesin light chain 1 isoforms.EMBO J. 2006; 25: 5457-5468Crossref PubMed Scopus (71) Google Scholar, 12.Woźniak M.J. Bola B. Brownhill K. Yang Y.C. Levakova V. Allan V.J. Role of kinesin-1 and cytoplasmic dynein in endoplasmic reticulum movement in VERO cells.J. Cell Sci. 2009; 122: 1979-1989Crossref PubMed Scopus (98) Google Scholar). The static interaction between ER membranes and MTs is mediated by several ER-residing proteins (6.Vedrenne C. Hauri H.-P. Morphogenesis of the endoplasmic reticulum: beyond active membrane expansion.Traffic. 2006; 7: 639-646Crossref PubMed Scopus (129) Google Scholar). We recently reported that the 42-kDa form of syntaxin 5 (Syn5L), which is generated by alternative initiation of translation and resides in the ER as well as the Golgi apparatus (13.Hui N. Nakamura N. Sönnichsen B. Shima D.T. Nilsson T. Warren G. An isoform of the Golgi t-SNARE, syntaxin 5, with an endoplasmic reticulum retrieval signal.Mol. Biol. Cell. 1997; 8: 1777-1787Crossref PubMed Scopus (80) Google Scholar), contributes to the organization of the ER structure by interacting with both cytoskeleton-linking membrane protein (CLIMP)-63/CKAP4 and MTs (14.Miyazaki K. Wakana Y. Noda C. Arasaki K. Furuno A. Tagaya M. Contribution of the long form of syntaxin 5 to the organization of the endoplasmic reticulum.J. Cell Sci. 2012; 125: 5658-5666Crossref PubMed Scopus (19) Google Scholar). CLIMP-63 is localized exclusively in the rough ER and mediates a static interaction with MTs (15.Klopfenstein D.R. Kappeler F. Hauri H.-P. A novel direct interaction of endoplasmic reticulum with microtubules.EMBO J. 1998; 17: 6168-6177Crossref PubMed Scopus (151) Google Scholar, 16.Klopfenstein D.R. Klumperman J. Lustig A. Kammerer R.A. Oorschot V. Hauri H.-P. Subdomain-specific localization of CLIMP-63 (p63) in the endoplasmic reticulum is mediated by its luminal α-helical segment.J. Cell Biol. 2001; 153: 1287-1300Crossref PubMed Scopus (102) Google Scholar, 17.Shibata Y. Shemesh T. Prinz W.A. Palazzo A.F. Kozlov M.M. Rapoport T.A. Mechanisms determining the morphology of the peripheral ER.Cell. 2010; 143: 774-788Abstract Full Text Full Text PDF PubMed Scopus (350) Google Scholar). Overexpression of Syn5L, as well as that of CLIMP-63 (15.Klopfenstein D.R. Kappeler F. Hauri H.-P. A novel direct interaction of endoplasmic reticulum with microtubules.EMBO J. 1998; 17: 6168-6177Crossref PubMed Scopus (151) Google Scholar), induces rearrangement and co-alignment of the ER membrane with MTs (14.Miyazaki K. Wakana Y. Noda C. Arasaki K. Furuno A. Tagaya M. Contribution of the long form of syntaxin 5 to the organization of the endoplasmic reticulum.J. Cell Sci. 2012; 125: 5658-5666Crossref PubMed Scopus (19) Google Scholar). This ER-MT bundling has been reported to be induced by overexpression of several MT-binding ER proteins (6.Vedrenne C. Hauri H.-P. Morphogenesis of the endoplasmic reticulum: beyond active membrane expansion.Traffic. 2006; 7: 639-646Crossref PubMed Scopus (129) Google Scholar, 18.Gao H. Sellin L.K. Pütz M. Nickel C. Imgrund M. Gerke P. Nitschke R. Walz G. Kramer-Zucker A.G. A short carboxy-terminal domain of polycystin-1 reorganizes the microtubular network and the endoplasmic reticulum.Exp. Cell Res. 2009; 315: 1157-1170Crossref PubMed Scopus (7) Google Scholar, 19.Ogawa-Goto K. Tanaka K. Ueno T. Tanaka K. Kurata T. Sata T. Irie S. p180 is involved in the interaction between the endoplasmic reticulum and microtubules through a novel microtubule-binding and bundling domain.Mol. Biol. Cell. 2007; 18: 3741-3751Crossref PubMed Scopus (55) Google Scholar), including Reep1 (20.Park S.H. Zhu P.P. Parker R.L. Blackstone C. Hereditary spastic paraplegia proteins REEP1, spastin, and atlastin-1 coordinate microtubule interactions with the tubular ER network.J. Clin. Invest. 2010; 120: 1097-1110Crossref PubMed Scopus (275) Google Scholar). Reep1, a member of the DP1/Yop1 superfamily, is predominantly localized in tubular smooth ER membranes (20.Park S.H. Zhu P.P. Parker R.L. Blackstone C. Hereditary spastic paraplegia proteins REEP1, spastin, and atlastin-1 coordinate microtubule interactions with the tubular ER network.J. Clin. Invest. 2010; 120: 1097-1110Crossref PubMed Scopus (275) Google Scholar). The DP1/Yop1, reticulon (RTN), and atlastin (ATL) families of proteins (6.Vedrenne C. Hauri H.-P. Morphogenesis of the endoplasmic reticulum: beyond active membrane expansion.Traffic. 2006; 7: 639-646Crossref PubMed Scopus (129) Google Scholar, 21.Hu J. Shibata Y. Zhu P.P. Voss C. Rismanchi N. Prinz W.A. Rapoport T.A. Blackstone C. A class of dynamin-like GTPases involved in the generation of the tubular ER network.Cell. 2009; 138: 549-561Abstract Full Text Full Text PDF PubMed Scopus (410) Google Scholar, 22.Orso G. Pendin D. Liu S. Tosetto J. Moss T.J. Faust J.E. Micaroni M. Egorova A. Martinuzzi A. McNew J.A. Daga A. Homotypic fusion of ER membranes requires the dynamin-like GTPase atlastin.Nature. 2009; 460: 978-983Crossref PubMed Scopus (341) Google Scholar) and the MT-severing protein spastin (23.Errico A. Ballabio A. Rugarli E.I. Spastin, the protein mutated in autosomal dominant hereditary spastic paraplegia, is involved in microtubule dynamics.Hum. Mol. Genet. 2002; 11: 153-163Crossref PubMed Scopus (284) Google Scholar) interact with one another and coordinate MT interactions with the tubular ER network (20.Park S.H. Zhu P.P. Parker R.L. Blackstone C. Hereditary spastic paraplegia proteins REEP1, spastin, and atlastin-1 coordinate microtubule interactions with the tubular ER network.J. Clin. Invest. 2010; 120: 1097-1110Crossref PubMed Scopus (275) Google Scholar). Valosin-containing protein (VCP)-interacting membrane protein (VIMP) was discovered as an ER-localized VCP/p97-interacting protein (24.Ye Y. Shibata Y. Yun C. Ron D. Rapoport T.A. A membrane protein complex mediates retro-translocation from the ER lumen into the cytosol.Nature. 2004; 429: 841-847Crossref PubMed Scopus (800) Google Scholar) and is alternatively called SelS because of its possession of selenocysteine at the penultimate amino acid residue (25.Shchedrina V.A. Zhang Y. Labunskyy V.M. Hatfield D.L. Gladyshev V.N. Structure-function relations, physiological roles, and evolution of mammalian ER-resident selenoproteins.Antioxid. Redox Signal. 2010; 12: 839-849Crossref PubMed Scopus (154) Google Scholar). The previous finding that overexpressed VIMP exhibits filamentous structures including ER membrane proteins (24.Ye Y. Shibata Y. Yun C. Ron D. Rapoport T.A. A membrane protein complex mediates retro-translocation from the ER lumen into the cytosol.Nature. 2004; 429: 841-847Crossref PubMed Scopus (800) Google Scholar) encouraged us to explore whether this protein, like CLIMP-63 (15.Klopfenstein D.R. Kappeler F. Hauri H.-P. A novel direct interaction of endoplasmic reticulum with microtubules.EMBO J. 1998; 17: 6168-6177Crossref PubMed Scopus (151) Google Scholar) and Syn5L (14.Miyazaki K. Wakana Y. Noda C. Arasaki K. Furuno A. Tagaya M. Contribution of the long form of syntaxin 5 to the organization of the endoplasmic reticulum.J. Cell Sci. 2012; 125: 5658-5666Crossref PubMed Scopus (19) Google Scholar), regulates ER structure by interacting with MTs and MT-binding proteins. In this study, we show that VIMP interacts with MTs and the rough ER protein CLIMP-63, but not with the smooth ER proteins, and regulates ER structure. Bacterially expressed GST-VIMP lacking the transmembrane domain was purified using glutathione beads and injected to rabbits. The antibody was affinity-purified using Sepharose 4B (GE Healthcare) coupled with His6-tagged VIMP lacking the transmembrane domain. A polyclonal anti-Bap31 antibody was prepared as reported previously (26.Wakana Y. Takai S. Nakajima K. Tani K. Yamamoto A. Watson P. Stephens D.J. Hauri H.-P. Tagaya M. Bap31 is an itinerant protein that moves between the peripheral endoplasmic reticulum (ER) and a juxtanuclear compartment related to ER-associated degradation.Mol. Biol. Cell. 2008; 19: 1825-1836Crossref PubMed Scopus (81) Google Scholar). A monoclonal antibody against CLIMP-63 was obtained from Enzo Life Science. Monoclonal antibodies against GM130 and calnexin were obtained from Pharmingen. Monoclonal and polyclonal antibodies against FLAG and α-tubulin were obtained from Sigma-Aldrich. An antiserum against Sec61β was purchased from Millipore Corp. A goat anti-RTN4 antibody and a rabbit anti-GST antibody were purchased from Santa Cruz Biotechnology. A polyclonal anti-VCP antibody was from Thermo Fisher Scientific. Brefeldin A (BFA), nocodazole, and Taxol were from Sigma-Aldrich. 293T cells were grown in Dulbecco's modified Eagle's medium supplemented with 50 IU/ml penicillin, 50 μg/ml streptomycin, and 10% fetal calf serum. HeLa cells were cultured in Eagle's minimum essential medium supplemented with the same materials. HeLa cells stably expressing monomeric red fluorescent protein (mRFP)-Sec61β were established previously (14.Miyazaki K. Wakana Y. Noda C. Arasaki K. Furuno A. Tagaya M. Contribution of the long form of syntaxin 5 to the organization of the endoplasmic reticulum.J. Cell Sci. 2012; 125: 5658-5666Crossref PubMed Scopus (19) Google Scholar). The cDNAs encoding VIMP and its truncation mutants were inserted into pFLAG-CMV-6c (Sigma-Aldrich). Construction of a Syn5L mutant and RTN family proteins was as reported previously (14.Miyazaki K. Wakana Y. Noda C. Arasaki K. Furuno A. Tagaya M. Contribution of the long form of syntaxin 5 to the organization of the endoplasmic reticulum.J. Cell Sci. 2012; 125: 5658-5666Crossref PubMed Scopus (19) Google Scholar, 27.Wakana Y. Koyama S. Nakajima K. Hatsuzawa K. Nagahama M. Tani K. Hauri H.-P. Melançon P. Tagaya M. Reticulon 3 is involved in membrane trafficking between the endoplasmic reticulum and Golgi.Biochem. Biophys. Res. Commun. 2005; 334: 1198-1205Crossref PubMed Scopus (73) Google Scholar). The cDNA encoding VIMP(48–187) was inserted into pGEX-4T-3. The cDNAs encoding Reep1/Reep5 and ATL1 (gifts from Dr. Craig Blackstone, NINDS, Bethesda, MD) were inserted into pFLAG-CMV-5a and pFLAG-CMV-6c, respectively, so as to express Reep1 and Reep5 each with a C-terminal FLAG tag and ATL1 with an N-terminal FLAG tag. Immunoprecipitation was performed as described previously (14.Miyazaki K. Wakana Y. Noda C. Arasaki K. Furuno A. Tagaya M. Contribution of the long form of syntaxin 5 to the organization of the endoplasmic reticulum.J. Cell Sci. 2012; 125: 5658-5666Crossref PubMed Scopus (19) Google Scholar). For immunoprecipitation of endogenous VIMP, 293T cells were homogenized in homogenization buffer (20 mm Hepes-KOH (pH 7.2), 150 mm KCl, 2 mm EDTA, 1 mm dithiothreitol, 10 μg/ml leupeptin, 1 μm pepstatin A, 2 μg/ml aprotinin, and 1 mm phenylmethylsulfonyl fluoride), and the membrane pellet was solubilized in homogenization buffer containing 1% Triton X-100. For immunoprecipitation of expressed proteins, 293T cells expressing FLAG-tagged proteins were lysed in homogenization buffer containing 1% Triton X-100. For immunofluorescence microscopy, cells were fixed with 4% paraformaldehyde (PFA) for 20 min at room temperature and observed with an Olympus FluoView 300 or 1000 laser scanning microscope as described (28.Tagaya M. Furuno A. Mizushima S. SNAP prevents Mg2+-ATP-induced release of N-ethylmaleimide-sensitive factor from the Golgi apparatus in digitonin-permeabilized PC12 cells.J. Biol. Chem. 1996; 271: 466-470Abstract Full Text Full Text PDF PubMed Scopus (44) Google Scholar). Immunoelectron microscopy was performed as described previously (29.Iinuma T. Aoki T. Arasaki K. Hirose H. Yamamoto A. Samata R. Hauri H.-P. Arimitsu N. Tagaya M. Tani K. Role of syntaxin 18 in the organization of endoplasmic reticulum subdomains.J. Cell Sci. 2009; 122: 1680-1690Crossref PubMed Scopus (43) Google Scholar), except that cells were fixed with 4% PFA for 30 min to retain immunoreactivity to VIMP. Subcellular fractionation was performed as described previously (30.Gilady S.Y. Bui M. Lynes E.M. Benson M.D. Watts R. Vance J.E. Simmen T. Ero1α requires oxidizing and normoxic conditions to localize to the mitochondria-associated membrane (MAM).Cell Stress Chaperones. 2010; 15: 619-629Crossref PubMed Scopus (134) Google Scholar). MT sedimentation assay was conducted as described previously (14.Miyazaki K. Wakana Y. Noda C. Arasaki K. Furuno A. Tagaya M. Contribution of the long form of syntaxin 5 to the organization of the endoplasmic reticulum.J. Cell Sci. 2012; 125: 5658-5666Crossref PubMed Scopus (19) Google Scholar) using porcine brain tubulin (Cytoskeleton, Inc.) After centrifugation, equal protein amounts of the supernatant and pellet fractions were subjected to SDS-PAGE and analyzed by immunoblotting with antibodies against GST and α-tubulin. The RNA duplexes used for targeting VIMP were VIMP(210) (5′-ggaaccugauguuguuguuaa-3′), VIMP(247) (5′-gcagcugcucgacugaaaau-3′), CLIMP-63(748) (5′-gacaacaucgccaucuuca-3′), Syn18(390) (5-caggaccgcuguuuuggauuu-3), and Syn5(390) (5′-ggaaauugaagagcuaaca-3′). The number in parentheses after each protein name indicates the position of the first nucleotide of siRNA relative to the start codon. All siRNAs were purchased from Japan Bioservice. Transfection was performed using Oligofectamine (Invitrogen) according to the manufacturer's protocol. The plasmid encoding ts045 VSVG-GFP was kindly supplied by Dr. Jennifer Lippincott-Schwartz (National Institutes of Health, Bethesda, MD). Assay was conducted as described previously (31.Presley J.F. Cole N.B. Schroer T.A. Hirschberg K. Zaal K.J. Lippincott-Schwartz J. ER-to-Golgi transport visualized in living cells.Nature. 1997; 389: 81-85Crossref PubMed Scopus (3) Google Scholar). VIMP is a 189-amino acid protein with one N-terminal transmembrane domain (amino acids 26–49) and luminal N-terminal and cytoplasmic C-terminal domains (Fig. 1A). A previous study showed that overexpression of VIMP altered ER morphology to filamentous structures (24.Ye Y. Shibata Y. Yun C. Ron D. Rapoport T.A. A membrane protein complex mediates retro-translocation from the ER lumen into the cytosol.Nature. 2004; 429: 841-847Crossref PubMed Scopus (800) Google Scholar). To examine whether MTs are involved in formation of the filamentous structure, we first overexpressed FLAG-VIMP(1–187) and compared the distribution of FLAG-VIMP-positive filaments with that of MTs. As shown in Fig. 1B, FLAG-VIMP caused the rearrangement of the ER, as seen by calnexin staining (upper panels), and MTs, as seen by α-tubulin staining (lower panels). This ER-MT-bundling pattern is almost the same as that observed in cells overexpressing CLIMP-63 (15.Klopfenstein D.R. Kappeler F. Hauri H.-P. A novel direct interaction of endoplasmic reticulum with microtubules.EMBO J. 1998; 17: 6168-6177Crossref PubMed Scopus (151) Google Scholar) and Syn5L (14.Miyazaki K. Wakana Y. Noda C. Arasaki K. Furuno A. Tagaya M. Contribution of the long form of syntaxin 5 to the organization of the endoplasmic reticulum.J. Cell Sci. 2012; 125: 5658-5666Crossref PubMed Scopus (19) Google Scholar). A similar ER-MT-bundling pattern was observed in cells expressing FLAG-tagged full-length VIMP (amino acids 1–189) (data not shown). Given that VIMP has an ER-MT-bundling activity, we next examined whether it interacts with CLIMP-63. As shown in Fig. 2A, CLIMP-63 coprecipitated with an anti-VIMP antibody (lane 2), whereas no coprecipitation was observed with a control IgG (lane 3). Of note is that α-tubulin did not coprecipitate with VIMP, excluding the possibility that the link between VIMP and CLIMP-63 is mediated through tubulins. To assess the importance of the link between VIMP and CLIMP-63, we knocked down CLIMP-63 by siRNA (Fig. 2B) and examined the formation of ER-MT bundling induced by VIMP overexpression. As shown in Fig. 2C, no clear bundling was detected in the absence of CLIMP-63 (middle panels), whereas bundling occurred in the absence of Syn5 (lower panels), which has been shown to bind to CLIMP-63 (14.Miyazaki K. Wakana Y. Noda C. Arasaki K. Furuno A. Tagaya M. Contribution of the long form of syntaxin 5 to the organization of the endoplasmic reticulum.J. Cell Sci. 2012; 125: 5658-5666Crossref PubMed Scopus (19) Google Scholar). We then examined the interaction of VIMP with polymerized MTs. To this end, a VIMP construct (amino acids 48–187) lacking the transmembrane domain was expressed as a GST fusion protein in Escherichia coli and purified. The purified protein was mixed with Taxol-stabilized MTs and then subjected to sedimentation as described under “Experimental Procedures.” As shown in Fig. 2D, almost all of GST-VIMP(48–187) (left, lane 6), but not GST (lane 3), coprecipitated with polymerized MTs. This coprecipitation did not occur in the absence of MTs (Fig. 2D, right, lane 6), suggesting the specific binding between VIMP and polymerized MTs. To confirm the interaction between VIMP and CLIMP-63, we expressed VIMP as a FLAG-tagged protein in 293T cells and performed immunoprecipitation. As shown in Fig. 3A (lane 12), CLIMP-63 (second panel), in addition to VCP (upper panel) and the long form of Syn5 (Syn5L) (third panel), coprecipitated with FLAG-VIMP(1–187). A similar precipitation pattern was observed for FLAG-tagged full-length VIMP (data not shown), suggesting that selenocysteine is not involved in the interaction with these proteins. Because Syn5L coprecipitated with FLAG-VIMP, we examined the endogenous interaction between Syn5 and VIMP. We found little coprecipitation of Syn5 with an anti-VIMP antibody (data not shown), suggesting that the interaction of VIMP with Syn5L is weak compared with that with CLIMP-63. To define the regions of VIMP responsible for the interactions with CLIMP-63 and Syn5, we produced a series of C-terminally truncated mutants. VIMP(1–175) showed a markedly decreased ability to bind to CLIMP-63, but retained Syn5L-binding ability (Fig. 3A, lane 11). VIMP(1–166) (lane 10), but not VIMP(1–146) (lane 9), was found to have Syn5L-binding activity. Essentially no binding to Syn5L or CLIMP-63 was observed for VIMP(1–73) (Fig. 3A, lane 8). The bundle-forming activity of VIMP appeared to be correlated with its CLIMP-63- and Syn5L-binding abilities. VIMP(1–175) and VIMP(1–166), both of which bound Syn5L but little or no CLIMP-63 (Fig. 3A, lanes 10 and 11), exhibited a bundling-staining pattern, but with a substantial background of diffuse staining (Fig. 3B, second and third rows). Bundling was not obvious for VIMP(1–146) (Fig. 3B, fourth row), which bound little or no CLIMP-63 or Syn5L (Fig. 3A, lane 9), and an ER- and/or plasma membrane-staining pattern was observed for VIMP(1–73) (Fig. 3B, lower row). Intuitively, the data in Fig. 3B (second and third rows) appear to be contradictory to the finding that CLIMP-63 is essential for ER-MT bundling (Fig. 2C). The VIMP constructs with very low CLIMP-63-binding activities could induce ER-MT bundling. This can be reconciled with the idea that CLIMP-63 is a principal ER-MT anchor protein, whereas VIMP and Syn5L are secondary ones. In the presence of endogenous CLIMP-63, the ER is fully attached to MTs; therefore, the interaction of overexpressed VIMP with endogenous Syn5L might be enough to form ER-MT bundles (Fig. 3B, second and third rows). In contrast, in the absence of CLIMP-63, the association of overexpressed VIMP with endogenous Syn5L might be insufficient for the formation of ER-MT bundles (Fig. 2C). To investigate whether VIMP is involved in the organization of the ER, we knocked down VIMP expression and examined the morphology of the ER. Both siRNA(210) and siRNA(247) effectively knocked down VIMP expression (Fig. 4A). Of note, no dispersal of a Golgi marker (GM130) was observed, suggesting that ER-to-Golgi transport is not impaired in VIMP-depleted cells. Consistent with this idea, the transport of VSVG-GFP from the ER to the plasma membrane through the Golgi apparatus was not significantly affected by VIMP depletion (Fig. 4B). In contrast, both siRNAs caused spreading of the ER membrane proteins Sec61β and CLIMP-63 to the cell periphery (Fig. 5A). These spreading patterns were similar to those in cells depleted of CLIMP-63 or Syn5 (14.Miyazaki K. Wakana Y. Noda C. Arasaki K. Furuno A. Tagaya M. Contribution of the long form of syntaxin 5 to the organization of the endoplasmic reticulum.J. Cell Sci. 2012; 125: 5658-5666Crossref PubMed Scopus (19) Google Scholar), suggesting the involvement of VIMP in shaping the ER.FIGURE 5.Knockdown of VIMP causes ER spreading. HeLa cells (A) or those stably expressing mRFP-Sec61β (B) were transfected without (Mock) or with one of the siRNAs targeting VIMP (siRNA(210) or siRNA(247)). A, after 72 h, the HeLa cells were fixed and stained with an antibody against Sec61β (left) or CLIMP-63 (right). Sec61β or CLIMP-63 fluorescence along white lines from the nucleus to the cell periphery was analyzed using ImageJ software. The average values of fluorescence brightness in five cells are shown. Scale bar = 5 μm. B, alternatively, HeLa cells stably expressing mRFP-Sec61β were incubated with 5 μg/ml nocodazole, and live cell imaging was performed. Representative images at the indicated times are shown. Arrows indicate peripheral regions, where ER structures were rarely seen before nocodazole treatment. Scale bar = 5 μm.View Large Image Figure ViewerDownload Hi-res image Download (PPT) In cells depleted of Syn5, the distribution of spread ER membranes was not affected by the MT-depolymerizing reagent nocodazole (14.Miyazaki K. Wakana Y. Noda C. Arasaki K. Furuno A. Tagaya M. Contribution of the long form of syntaxin 5 to the organization of the endoplasmic reticulum.J. Cell Sci. 2012; 125: 5658-5666Crossref PubMed Scopus (19) Google Scholar). To show that depletion of VIMP specifically affects ER structure, not cell shape, we treated mRFP-Sec61β-expressing cells with nocodazole and performed live cell imaging. As shown in Fig. 5B, nocodazole treatment of VIMP-depleted cells did not cause further mRFP-Sec61β spreading, whereas the centrally accumulated mRFP-Sec61β in mock-treated cells was redistributed to more peripheral regions upon nocodazole treatment. Our previous study showed that an ER membrane protein, Bap31, undergoes one-time cycling between the peripheral and perinuclear ER regions in an MT-dependent manner after treatment with BFA, an inhibitor of the ARF1 (ADP-ribosylation factor 1) guanine nucleotide exchange factor (32.Donaldson J.G. Finazzi D. Klausner R.D. Brefeldin A inhibits Golgi membrane-catalysed exchange of guanine nucleotide onto ARF protein.Nature. 1992; 360: 350-352Crossref PubMed Scopus (595) Google Scholar), followed by its washout (26.Wakana Y. Takai S. Nakajima K. Tani K. Yamamoto A. Watson P. Stephens D.J. Hauri H.-P. Tagaya M. Bap31 is an itinerant protein that moves between the peripheral endoplasmic reticulum (ER) and a juxtanuclear compartment related to ER-associated degradation.Mol. Biol. Cell. 2008; 19: 1825-1836Crossref PubMed Scopus (81) Google Scholar). This may reflect the fact that the movement of Bap31 to the perinuclear region within the ER is ARF1-dependent (26.Wakana Y. Takai S. Nakajima K. Tani K. Yamamoto A. Watson P. Stephens D.J. Hauri H.-P. Tagaya M. Bap31 is an itinerant protein that moves between the peripheral endoplasmic reticulum (ER) and a juxtanuclear compartment related to ER-associated degradation.Mol. Biol. Cell. 2008; 19: 1825-1836Crossref PubMed Scopus (81) Google Scholar). To explore the link between VIMP and an MT-dependent process, we investigated the effect of VIMP depletion on the cycling of Bap31 within the ER. In mock-treated cells, BFA treatment/washout caused the movement of Bap31 to the perinuclear region at 120–240 min after BFA washout (Fig. 6, left), and the accumulated Bap31 returned to the cell periphery by 360 min in a considerable fraction of cells (lower row). In contrast, in VIMP-depleted cells, Bap31 exhibited large punctate peripheral staining at 120 min after BFA washout and did not accumulate at the perinuclear region (Fig. 6, right). At 360 min after BFA washout, Bap31 remained as punctuate structures in many cells (Fig. 6, lower row). These results suggest that the MT- and dynein-dependent movement of Bap31 to the perinuclear region is inhibited by VIMP depletion. Our previous study showed that Lys-82 of Syn5L is important for binding to CLIMP-63 and MTs (14.Miyazaki K. Wakana Y. Noda C. Arasaki K. Furuno A. Tagaya M. Contribution of the long form of syntaxin 5 to the organization of the endoplasmic reticulum.J. Cell Sci. 2012; 125: 5658-5666Crossref PubMed Scopus (19) Google Scholar). As shown in Fig. 7A, this residue is also responsible for the interaction with VIMP. Next, we examined whether VIMP interacts with proteins (such as the RTN, DP1/Yop1, and ATL families) that are responsible for shaping the smooth ER (20.Park S.H. Zhu P.P. Parker R.L. Blackstone C. Hereditary spastic paraplegia proteins REEP1, spastin, and atlastin-1 coordinate microtubule interactions with the tubular ER network.J. Clin. Invest. 2010; 120: 1097-1110Crossref PubMed Scopus (275) Google Scholar, 21.Hu J. Shibata Y. Zhu P.P. Voss C. Rismanchi N. Prinz W.A. Rapoport T.A. Blackstone C. A class of dynamin-like GTPases involved in the generation of the tubular ER network.Cell. 2009; 138: 549-561Abstract Full Text Full Text PDF PubMed Scopus (410) Google Scholar, 33.Voeltz G.K. Prinz W.A. Shibata Y. Rist J.M. Rapoport T.A. A class of membrane proteins shaping the tubular endoplasmic reticulum.Cell. 2006; 124: 573-586Abstract Full Text Full Text PDF PubMed Scopus (850) Google Scholar). These protein families and the MT-severing protein spastin interact with one another and coordinate MT interactions with the tubular ER network (20.Park S.H. Zhu P.P. Parker R.L. Blackstone C. Hereditary spastic paraplegia proteins REEP1, spastin, and atlastin-1 coordinate microtubule interactions with the tubular ER network.J. Clin. Invest. 2010; 120: 1097-1110Crossref PubMed Scopus (275) Google Scholar). As shown in Fig. 7 (B and C), Reep family members (Reep1 and Reep5 each represent two distinct Reep1 subfamilies (20.Park S.H. Zhu P.P. Parker R.L. Blackstone C. Hereditary spastic paraplegia proteins REEP1, spastin, and atlastin-1 coordinate microtubule interactions with the tubular ER network.J. Clin. Invest. 2010; 120: 1097-1110Crossref PubMed Scopus (275) Google Scholar)), ATL1, RTN3, RTN4a, RTN4b, and RTN4c were found not to bind to FLAG-VIMP. To determine the distribution of VIMP in the ER, we first performed subcellular fractionation using a 5–25% OptiPrep gradient. In our protocol, VIMP almost completely co-sedimented with CLIMP-63 with a peak in fractions 6 and 7, whereas the smooth ER marker RTN4 sedimented in fractions 4–9 without a clear peak (Fig. 8A). Next, we sought to examine the distribution of VIMP by immunoelectron microscopy. Because the immunoreactivity of our antibody to VIMP was lost upon fixation of cells with 4% PFA plus 0.1% glutaraldehyde for 30 min or with 4% PFA for 2 h, we observed cells fixed with 4% PFA for 30 min. Because of mild fixation, the ultrastructure was slightly obscure, but the presence of VIMP on ER membranes was seen (Fig. 8B, arrows). As we could not clearly demonstrate the presence of VIMP on the rough ER, we examined whether VIMP behaves like rough ER membrane proteins upon Syn18 depletion. Our previous work showed that knockdown of Syn18, a SNARE localized in the ER (34.Hatsuzawa K. Hirose H. Tani K. Yamamoto A. Scheller R.H. Tagaya M. Syntaxin 18, a SNAP receptor that functions in the endoplasmic reticulum, intermediate compartment, and cis-Golgi vesicle trafficking.J. Biol. Chem. 2000; 275: 13713-13720Abstract Full Text Full Text PDF PubMed Scopus (96) Google Scholar), causes segregation of rough and smooth ER membranes (29.Iinuma T. Aoki T. Arasaki K. Hirose H. Yamamoto A. Samata R. Hauri H.-P. Arimitsu N. Tagaya M. Tani K. Role of syntaxin 18 in the organization of endoplasmic reticulum subdomains.J. Cell Sci. 2009; 122: 1680-1690Crossref PubMed Scopus (43) Google Scholar), although the precise mechanism underlying this phenomenon remains unclear. In Syn18-depleted cells, smooth ER membrane proteins such as RTN4 aggregate to form patch-like structures, whereas rough ER membrane proteins such as CLIMP-63 and Sec61β remain largely unchanged and are almost completely excluded from the patch-like structures (29.Iinuma T. Aoki T. Arasaki K. Hirose H. Yamamoto A. Samata R. Hauri H.-P. Arimitsu N. Tagaya M. Tani K. Role of syntaxin 18 in the organization of endoplasmic reticulum subdomains.J. Cell Sci. 2009; 122: 1680-1690Crossref PubMed Scopus (43) Google Scholar). We knocked down Syn18 and stained cells with antibodies against VIMP and smooth or rough ER markers. In mock-treated cells, the distribution of all proteins examined was almost indistinguishable at the level of immunofluorescence microscopy (Fig. 9, A and B, left). As reported previously (29.Iinuma T. Aoki T. Arasaki K. Hirose H. Yamamoto A. Samata R. Hauri H.-P. Arimitsu N. Tagaya M. Tani K. Role of syntaxin 18 in the organization of endoplasmic reticulum subdomains.J. Cell Sci. 2009; 122: 1680-1690Crossref PubMed Scopus (43) Google Scholar), in Syn18-depleted cells, RTN4 became aggregated, whereas CLIMP-63 and Sec61β largely retained their distributions except for the absence in RTN4-positive patches (Fig. 9A, right). Similarly, VIMP did not accumulate at the aggregates and largely retained normal morphology (Fig. 9B, right), consistent with the finding that VIMP specifically interacts with rough ER membrane proteins. MTs play an important role in the organization of the ER in mammalian cells. The static interaction between ER membranes and MTs is mediated by several ER-residing proteins (6.Vedrenne C. Hauri H.-P. Morphogenesis of the endoplasmic reticulum: beyond active membrane expansion.Traffic. 2006; 7: 639-646Crossref PubMed Scopus (129) Google Scholar). We have recently demonstrated that the ER-residing form of Syn5 (Syn5L) regulates ER structure by interacting with both CLIMP-63 and MTs (14.Miyazaki K. Wakana Y. Noda C. Arasaki K. Furuno A. Tagaya M. Contribution of the long form of syntaxin 5 to the organization of the endoplasmic reticulum.J. Cell Sci. 2012; 125: 5658-5666Crossref PubMed Scopus (19) Google Scholar). In the present study, we have shown that VIMP also contributes to the organization of the ER by interacting with both CLIMP-63 and MTs. It should be noted that VIMP binds to polymerized MTs (Fig. 2D), but not depolymerized tubulin (Fig. 2A), suggesting that VIMP directly interacts with CLIMP-63, not through tubulin. As in the case of Syn5L (14.Miyazaki K. Wakana Y. Noda C. Arasaki K. Furuno A. Tagaya M. Contribution of the long form of syntaxin 5 to the organization of the endoplasmic reticulum.J. Cell Sci. 2012; 125: 5658-5666Crossref PubMed Scopus (19) Google Scholar), ER-MT bundle formation induced by VIMP overexpression is dependent on the presence of CLIMP-63. Mutation analysis revealed a rough correlation between bundle formation and abilities to interact with CLIMP-63 and Syn5L. Of note, Lys-82 of Syn5L is responsible for the interaction with VIMP as well as with CLIMP-63 and MTs (14.Miyazaki K. Wakana Y. Noda C. Arasaki K. Furuno A. Tagaya M. Contribution of the long form of syntaxin 5 to the organization of the endoplasmic reticulum.J. Cell Sci. 2012; 125: 5658-5666Crossref PubMed Scopus (19) Google Scholar). Perhaps multivalent and cooperative interactions of VIMP with MT-binding proteins and MTs are necessary for ER-MT bundle formation. It should be emphasized that CLIMP-63 serves as a critical hub for MT-binding ER proteins. Depletion of VIMP caused spreading of the ER membrane, a characteristic phenotype observed when the link between the ER and MTs is disrupted by nocodazole treatment (14.Miyazaki K. Wakana Y. Noda C. Arasaki K. Furuno A. Tagaya M. Contribution of the long form of syntaxin 5 to the organization of the endoplasmic reticulum.J. Cell Sci. 2012; 125: 5658-5666Crossref PubMed Scopus (19) Google Scholar, 35.Lu L. Ladinsky M.S. Kirchhausen T. Cisternal organization of the endoplasmic reticulum during mitosis.Mol. Biol. Cell. 2009; 20: 3471-3480Crossref PubMed Scopus (146) Google Scholar). A difference in the effects of nocodazole treatment and VIMP depletion is that the Golgi apparatus is disassembled by MT depolymerization, whereas no Golgi disassembly is induced by VIMP depletion. In addition to a morphological change in the ER, VIMP depletion affected one round of MT-dependent Bap31 cycling after BFA washout. These results suggest that VIMP is involved in MT-dependent processes on the ER, but not in other pathways such as ER-to-Golgi transport and the maintenance of the steady-state distribution of GM130. Like CLIMP-63 (21.Hu J. Shibata Y. Zhu P.P. Voss C. Rismanchi N. Prinz W.A. Rapoport T.A. Blackstone C. A class of dynamin-like GTPases involved in the generation of the tubular ER network.Cell. 2009; 138: 549-561Abstract Full Text Full Text PDF PubMed Scopus (410) Google Scholar), VIMP was found not to interact with smooth ER-shaping proteins. Moreover, our data show that VIMP did not accumulate in smooth ER patches in Syn18-depleted cells, although we could not clearly demonstrate that VIMP is localized in rough ER membranes by immunoelectron microscopy. Blackstone and co-workers (20.Park S.H. Zhu P.P. Parker R.L. Blackstone C. Hereditary spastic paraplegia proteins REEP1, spastin, and atlastin-1 coordinate microtubule interactions with the tubular ER network.J. Clin. Invest. 2010; 120: 1097-1110Crossref PubMed Scopus (275) Google Scholar) suggested the possibility that Reep1 and CLIMP-63 play similar roles in mediating ER-MT interactions in different ER subdomains. Extending their idea, we have hypothesized that multivalent and cooperative interactions between ER membrane proteins, such as CLIMP-63 (15.Klopfenstein D.R. Kappeler F. Hauri H.-P. A novel direct interaction of endoplasmic reticulum with microtubules.EMBO J. 1998; 17: 6168-6177Crossref PubMed Scopus (151) Google Scholar, 16.Klopfenstein D.R. Klumperman J. Lustig A. Kammerer R.A. Oorschot V. Hauri H.-P. Subdomain-specific localization of CLIMP-63 (p63) in the endoplasmic reticulum is mediated by its luminal α-helical segment.J. Cell Biol. 2001; 153: 1287-1300Crossref PubMed Scopus (102) Google Scholar), Syn5L (14.Miyazaki K. Wakana Y. Noda C. Arasaki K. Furuno A. Tagaya M. Contribution of the long form of syntaxin 5 to the organization of the endoplasmic reticulum.J. Cell Sci. 2012; 125: 5658-5666Crossref PubMed Scopus (19) Google Scholar), Syn5-interacting proteins (polycystins (36.Geng L. Boehmerle W. Maeda Y. Okuhara D.Y. Tian X. Yu Z. Choe C.U. Anyatonwu G.I. Ehrlich B.E. Somlo S. Syntaxin 5 regulates the endoplasmic reticulum channel-release properties of polycystin-2.Proc. Natl. Acad. Sci. U.S.A. 2008; 105: 15920-15925Crossref PubMed Scopus (54) Google Scholar)), and those between these proteins and MTs are required for the organization of the rough ER (14.Miyazaki K. Wakana Y. Noda C. Arasaki K. Furuno A. Tagaya M. Contribution of the long form of syntaxin 5 to the organization of the endoplasmic reticulum.J. Cell Sci. 2012; 125: 5658-5666Crossref PubMed Scopus (19) Google Scholar). Our present results reveal that VIMP is such an ER-shaping protein. We thank Naohiko Hirota and Yuki Konno for technical assistance and Drs. Craig Blackstone and Jennifer Lippincott-Schwartz for kind gifts of plasmids." @default.
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• W2045878597 title "Valosin-containing Protein-interacting Membrane Protein (VIMP) Links the Endoplasmic Reticulum with Microtubules in Concert with Cytoskeleton-linking Membrane Protein (CLIMP)-63" @default.
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