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• W2045879251 endingPage "e1002305" @default.
• W2045879251 startingPage "e1002305" @default.
• W2045879251 abstract "Meiotic DNA double-strand breaks (DSBs) initiate crossover (CO) recombination, which is necessary for accurate chromosome segregation, but DSBs may also repair as non-crossovers (NCOs). Multiple recombination pathways with specific intermediates are expected to lead to COs and NCOs. We revisited the mechanisms of meiotic DSB repair and the regulation of CO formation, by conducting a genome-wide analysis of strand-transfer intermediates associated with recombination events. We performed this analysis in a SK1 × S288C Saccharomyces cerevisiae hybrid lacking the mismatch repair (MMR) protein Msh2, to allow efficient detection of heteroduplex DNAs (hDNAs). First, we observed that the anti-recombinogenic activity of MMR is responsible for a 20% drop in CO number, suggesting that in MMR-proficient cells some DSBs are repaired using the sister chromatid as a template when polymorphisms are present. Second, we observed that a large fraction of NCOs were associated with trans-hDNA tracts constrained to a single chromatid. This unexpected finding is compatible with dissolution of double Holliday junctions (dHJs) during repair, and it suggests the existence of a novel control point for CO formation at the level of the dHJ intermediate, in addition to the previously described control point before the dHJ formation step. Finally, we observed that COs are associated with complex hDNA patterns, confirming that the canonical double-strand break repair model is not sufficient to explain the formation of most COs. We propose that multiple factors contribute to the complexity of recombination intermediates. These factors include repair of nicks and double-stranded gaps, template switches between non-sister and sister chromatids, and HJ branch migration. Finally, the good correlation between the strand transfer properties observed in the absence of and in the presence of Msh2 suggests that the intermediates detected in the absence of Msh2 reflect normal intermediates." @default.
• W2045879251 created "2016-06-24" @default.
• W2045879251 creator A5003525592 @default.
• W2045879251 creator A5019531434 @default.
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• W2045879251 creator A5046206406 @default.
• W2045879251 creator A5049852238 @default.
• W2045879251 creator A5056148043 @default.
• W2045879251 creator A5062584758 @default.
• W2045879251 date "2011-09-29" @default.
• W2045879251 modified "2023-10-17" @default.
• W2045879251 title "Genome-Wide Analysis of Heteroduplex DNA in Mismatch Repair–Deficient Yeast Cells Reveals Novel Properties of Meiotic Recombination Pathways" @default.
• W2045879251 cites W1491342176 @default.
• W2045879251 cites W1663920100 @default.
• W2045879251 cites W1787998249 @default.
• W2045879251 cites W1833842636 @default.
• W2045879251 cites W1848421747 @default.
• W2045879251 cites W1893937409 @default.
• W2045879251 cites W1903900184 @default.
• W2045879251 cites W1921105195 @default.
• W2045879251 cites W1964714687 @default.
• W2045879251 cites W1971201699 @default.
• W2045879251 cites W1974440260 @default.
• W2045879251 cites W1981372238 @default.
• W2045879251 cites W1982893758 @default.
• W2045879251 cites W1984829206 @default.
• W2045879251 cites W1986730363 @default.
• W2045879251 cites W1988875152 @default.
• W2045879251 cites W1991624307 @default.
• W2045879251 cites W1991744923 @default.
• W2045879251 cites W2001396107 @default.
• W2045879251 cites W2001680897 @default.
• W2045879251 cites W2002454928 @default.
• W2045879251 cites W2008144158 @default.
• W2045879251 cites W2012838063 @default.
• W2045879251 cites W2013737559 @default.
• W2045879251 cites W2013818568 @default.
• W2045879251 cites W2016505307 @default.
• W2045879251 cites W2016544300 @default.
• W2045879251 cites W2018845677 @default.
• W2045879251 cites W2019551788 @default.
• W2045879251 cites W2019661860 @default.
• W2045879251 cites W2026175627 @default.
• W2045879251 cites W2034252391 @default.
• W2045879251 cites W2035792235 @default.
• W2045879251 cites W2037019891 @default.
• W2045879251 cites W2039316942 @default.
• W2045879251 cites W2039516370 @default.
• W2045879251 cites W2045349139 @default.
• W2045879251 cites W2050060181 @default.
• W2045879251 cites W2056885612 @default.
• W2045879251 cites W2066792579 @default.
• W2045879251 cites W2066976424 @default.
• W2045879251 cites W2067331893 @default.
• W2045879251 cites W2068119185 @default.
• W2045879251 cites W2068246844 @default.
• W2045879251 cites W2068317960 @default.
• W2045879251 cites W2068480338 @default.
• W2045879251 cites W2070650108 @default.
• W2045879251 cites W2071106544 @default.
• W2045879251 cites W2075152713 @default.
• W2045879251 cites W2075319181 @default.
• W2045879251 cites W2080408951 @default.
• W2045879251 cites W2082447947 @default.
• W2045879251 cites W2082471729 @default.
• W2045879251 cites W2095835610 @default.
• W2045879251 cites W2101769299 @default.
• W2045879251 cites W2114700051 @default.
• W2045879251 cites W2115367924 @default.
• W2045879251 cites W2116471216 @default.
• W2045879251 cites W2123960763 @default.
• W2045879251 cites W2126187754 @default.
• W2045879251 cites W2127513751 @default.
• W2045879251 cites W2134357164 @default.
• W2045879251 cites W2134556593 @default.
• W2045879251 cites W2135657415 @default.
• W2045879251 cites W2136144795 @default.
• W2045879251 cites W2137148725 @default.
• W2045879251 cites W2138791190 @default.
• W2045879251 cites W2140824976 @default.
• W2045879251 cites W2143542418 @default.
• W2045879251 cites W2144550200 @default.
• W2045879251 cites W2145015866 @default.
• W2045879251 cites W2145982363 @default.
• W2045879251 cites W2147946762 @default.
• W2045879251 cites W2153820061 @default.
• W2045879251 cites W2155237411 @default.
• W2045879251 cites W2159487855 @default.
• W2045879251 cites W2160354893 @default.
• W2045879251 cites W2160563322 @default.
• W2045879251 cites W2162262302 @default.
• W2045879251 cites W2164936754 @default.
• W2045879251 cites W2165870010 @default.
• W2045879251 cites W2166018709 @default.
• W2045879251 cites W2170200928 @default.
• W2045879251 cites W2170366746 @default.
• W2045879251 cites W2170392103 @default.

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