Matches in SemOpenAlex for { <https://semopenalex.org/work/W2045932408> ?p ?o ?g. }
Showing items 1 to 64 of
64
with 100 items per page.
- W2045932408 endingPage "le23" @default.
- W2045932408 startingPage "le23" @default.
- W2045932408 abstract "Reading this paper (1) carefully, we do not find that “S100A4 regulates IL-1β-dependent induction of MMP-13,” as stated in the title, is convincingly demonstrated. Thorough interpretation of the data raises several compelling concerns, and we doubt conclusions drawn based on comments specified below.The authors state that “the immunofluorescence data (Fig. 1A) showed that under basal conditions S100A4 was predominantly localized in the cytoplasm.” We agree that the red channel (middle row, Fig. 1A) shows S100A4 at all time points, and in Fig. 1B cytoplasmic staining is equally strong at all time points. Therefore, why is cytoplasmic staining of S100A4 absent at time 0 and 120 min in the overlay image?In Fig. 2B, why does the SUMO antibody pick up S100A4 that is not SUMOylated, and could the claimed SUMO-S100A4 be instead a multimeric form of SUMO?For clarification, basal levels of endogenous and extracellular S100A4 in chondrocytes should be included in Figs. 3B and 4C, respectively.In our opinion, Fig. 4, A and B, lacks the IL-1β-treated pEGFP empty vector-transfected control. This is, however, included in Fig. 4D and gives an ∼2.25-fold increase in MMP-13 mRNA. When transferring these data into Fig. 4B and comparing it to the ∼2.75-fold increase induced by overexpressed S100A4, a significant difference is not evident. Moreover, in Fig. 4D, no p value or comment is given on the effect of IL-1β treatment alone (bar 1 versus 4) compared to overexpressed S100A4-GFP (bar 2 versus 5).Why does the effect of IL-1β on S100A4-GFP-transfected cells change from ∼2.75-fold in Fig. 4A (bar 2) to ∼4-fold in Fig. 4B (bar 5)?" @default.
- W2045932408 created "2016-06-24" @default.
- W2045932408 creator A5002092097 @default.
- W2045932408 creator A5003610591 @default.
- W2045932408 creator A5053950619 @default.
- W2045932408 creator A5066128117 @default.
- W2045932408 date "2010-12-01" @default.
- W2045932408 modified "2023-09-28" @default.
- W2045932408 title "Comment on the Importance of S100A4 in Regulation of MMP-13" @default.
- W2045932408 cites W2002255802 @default.
- W2045932408 doi "https://doi.org/10.1074/jbc.l110.125898" @default.
- W2045932408 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3009943" @default.
- W2045932408 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21186294" @default.
- W2045932408 hasPublicationYear "2010" @default.
- W2045932408 type Work @default.
- W2045932408 sameAs 2045932408 @default.
- W2045932408 citedByCount "2" @default.
- W2045932408 countsByYear W20459324082017 @default.
- W2045932408 crossrefType "journal-article" @default.
- W2045932408 hasAuthorship W2045932408A5002092097 @default.
- W2045932408 hasAuthorship W2045932408A5003610591 @default.
- W2045932408 hasAuthorship W2045932408A5053950619 @default.
- W2045932408 hasAuthorship W2045932408A5066128117 @default.
- W2045932408 hasBestOaLocation W20459324082 @default.
- W2045932408 hasConcept C109523444 @default.
- W2045932408 hasConcept C12554922 @default.
- W2045932408 hasConcept C15744967 @default.
- W2045932408 hasConcept C169760540 @default.
- W2045932408 hasConcept C185592680 @default.
- W2045932408 hasConcept C55493867 @default.
- W2045932408 hasConcept C86803240 @default.
- W2045932408 hasConcept C95444343 @default.
- W2045932408 hasConceptScore W2045932408C109523444 @default.
- W2045932408 hasConceptScore W2045932408C12554922 @default.
- W2045932408 hasConceptScore W2045932408C15744967 @default.
- W2045932408 hasConceptScore W2045932408C169760540 @default.
- W2045932408 hasConceptScore W2045932408C185592680 @default.
- W2045932408 hasConceptScore W2045932408C55493867 @default.
- W2045932408 hasConceptScore W2045932408C86803240 @default.
- W2045932408 hasConceptScore W2045932408C95444343 @default.
- W2045932408 hasIssue "53" @default.
- W2045932408 hasLocation W20459324081 @default.
- W2045932408 hasLocation W20459324082 @default.
- W2045932408 hasLocation W20459324083 @default.
- W2045932408 hasLocation W20459324084 @default.
- W2045932408 hasOpenAccess W2045932408 @default.
- W2045932408 hasPrimaryLocation W20459324081 @default.
- W2045932408 hasRelatedWork W1437588494 @default.
- W2045932408 hasRelatedWork W1754220768 @default.
- W2045932408 hasRelatedWork W2091139028 @default.
- W2045932408 hasRelatedWork W2156234950 @default.
- W2045932408 hasRelatedWork W2746421487 @default.
- W2045932408 hasRelatedWork W2748952813 @default.
- W2045932408 hasRelatedWork W287587132 @default.
- W2045932408 hasRelatedWork W2899084033 @default.
- W2045932408 hasRelatedWork W3029784168 @default.
- W2045932408 hasRelatedWork W74938519 @default.
- W2045932408 hasVolume "285" @default.
- W2045932408 isParatext "false" @default.
- W2045932408 isRetracted "false" @default.
- W2045932408 magId "2045932408" @default.
- W2045932408 workType "article" @default.