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• W2045941738 abstract "X inactivation is effected by a large <i>cis</i>-acting RNA molecule termed the X inactive specific transcript <i>(Xist)</i>. Exon IV of <i>Xist</i> RNA is highly conserved at the primary sequence level and is predicted to form a stable stem-loop structure. These features suggest that it is important for <i>Xist</i> RNA function. We have used homologous recombination to delete exon IV of the mouse <i>Xist</i> gene. Surprisingly we found no detectable effects on X inactivation. Heterozygous female animals show normal random X inactivation and transcripts from the mutant allele were seen to localise <i>in cis</i> over the length of the inactive X chromosome. There was however a reduced steady state level of mutant relative to wild type <i>Xist</i> RNA. This effect was not attributable to decreased stability, suggesting that the deletion affects transcription or processing of <i>Xist</i> RNA." @default.
• W2045941738 created "2016-06-24" @default.
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• W2045941738 date "2002-01-01" @default.
• W2045941738 modified "2023-10-17" @default.
• W2045941738 title "Functional analysis of the highly conserved exon IV of <i>Xist</i> RNA" @default.
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• W2045941738 doi "https://doi.org/10.1159/000071580" @default.
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