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• W2045950745 abstract "(1 → 4)-N-Acetyl-β-d-galactosaminyltransferase (GalNAcT-1) and (1 → 3)-N-acetyl-β-d-galacatosaminyltranferase (GalNAcT-2), which are involved in the in vitro biosynthesis of GM2 and GbOse4Cer glycosphingolipids, respectively, have been solubilized and separated by differential detergent extraction from a membrane preparation of 19-day-old embryonic chicken brain. The separated GalNAcT-1 activity had a pH optima of 7.8–8.0, and the separated GalNAcT-2 activity a single pH optimum of 7.2. Furthermore, the partially purified GalNAcT-2 preparation catalyzed the transfer of N-acetylgalactosamine from UDP-d-[3H]GalNAc to only GbOse3Cer and nLcOse5Cer. Both GalNAcT-1 and GalNAcT-2 activities were purified to ∼ 316- and 428-fold, respectively, by use of UDP-hexanolamine-Sepharose 4B affinity-column chromatography. However, the partially purified GalNAcT-1 preparation appeared to be active only with GM3, lactosylceramide, and lactotriaosylceramide. The proposed linkage of the N-acetylgalactosamine unit incorporated into GM3 is β-d-GalpNAc-(1 → 4)-GM3 from the isolation of [3H]threitol after hydrolysis of the desialylated, lead tetraacetate-treated, enzymic product, β-d-GalpNAc-(1 → 4)-β-d-[6-3H]Galp-(1 → 4)-β-d-Glcp-(1 → 1)-Cer. In addition, β-d-GalpNAc-(1 → 3)-GbOse3Cer was produced, as shown by the identification of 2,4,6-tri-O-methylgalactose after permethylation and hydrolysis of the GalNAcT-2 enzymic product, GalpNAc-[6-3H]Galp- → Gal → Glc → Cer." @default.
• W2045950745 created "2016-06-24" @default.
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• W2045950745 date "1992-12-01" @default.
• W2045950745 modified "2023-10-16" @default.
• W2045950745 title "In vitro biosynthesis of GbOse4Cer (globoside) and GM2 ganglioside by the (1 → 3) and (1 → 4)-N-acetyl β-d-galactosaminyltransferases from embryonic chicken brain. Solubilization, purification, and characterization of the transferases" @default.
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• W2045950745 doi "https://doi.org/10.1016/0008-6215(92)85018-u" @default.
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