FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2045952105> ?p ?o ?g. }

• W2045952105 endingPage "3030" @default.
• W2045952105 startingPage "3020" @default.
• W2045952105 abstract "ABSTRACT The human immunodeficiency virus type 1 (HIV-1) particle contains two identical RNA strands, each corresponding to the entire genome. The 5′ untranslated region (UTR) of each RNA strand contains extensive secondary and tertiary structures that are instrumental in different steps of the viral replication cycle. We have characterized the 5′ UTRs of nine different HIV-1 isolates representing subtypes A through G and, by comparing their homodimerization and heterodimerization potentials, found that complementarity between the palindromic sequences in the dimerization initiation site (DIS) hairpins is necessary and sufficient for in vitro dimerization of two subtype RNAs. The 5′ UTR sequences were used to design donor and acceptor templates for a coupled in vitro dimerization-reverse transcription assay. We showed that template switching during reverse transcription is increased with a matching DIS palindrome and further stimulated proportional to the level of homology between the templates. The presence of the HIV-1 nucleocapsid protein NCp7 increased the template-switching efficiency for matching DIS palindromes twofold, whereas the recombination efficiency was increased sevenfold with a nonmatching palindrome. Since NCp7 did not effect the dimerization of nonmatching palindromes, we concluded that the protein most likely stimulates the strand transfer reaction. An analysis of the distribution of template-switching events revealed that it occurs throughout the 5′ UTR. Together, these results demonstrate that the template switching of HIV-1 reverse transcriptase occurs frequently in vitro and that this process is facilitated mainly by template proximity and the level of homology." @default.
• W2045952105 created "2016-06-24" @default.
• W2045952105 creator A5019287445 @default.
• W2045952105 creator A5058499082 @default.
• W2045952105 creator A5072940644 @default.
• W2045952105 creator A5090186785 @default.
• W2045952105 creator A5091604665 @default.
• W2045952105 date "2003-03-01" @default.
• W2045952105 modified "2023-10-17" @default.
• W2045952105 title "Dimerization and Template Switching in the 5′ Untranslated Region between Various Subtypes of Human Immunodeficiency Virus Type 1" @default.
• W2045952105 cites W1480821243 @default.
• W2045952105 cites W1495312602 @default.
• W2045952105 cites W1547414471 @default.
• W2045952105 cites W1711343352 @default.
• W2045952105 cites W1819107170 @default.
• W2045952105 cites W1870661610 @default.
• W2045952105 cites W1955113362 @default.
• W2045952105 cites W1973901059 @default.
• W2045952105 cites W1980779739 @default.
• W2045952105 cites W1981307279 @default.
• W2045952105 cites W1982297728 @default.
• W2045952105 cites W1985665571 @default.
• W2045952105 cites W2001534158 @default.
• W2045952105 cites W2001939628 @default.
• W2045952105 cites W2012148532 @default.
• W2045952105 cites W2020790990 @default.
• W2045952105 cites W2022824829 @default.
• W2045952105 cites W2027298939 @default.
• W2045952105 cites W2032467322 @default.
• W2045952105 cites W2039724561 @default.
• W2045952105 cites W2045074917 @default.
• W2045952105 cites W2054506114 @default.
• W2045952105 cites W2059513540 @default.
• W2045952105 cites W2061476669 @default.
• W2045952105 cites W2064475194 @default.
• W2045952105 cites W2067145794 @default.
• W2045952105 cites W2070911609 @default.
• W2045952105 cites W2071941492 @default.
• W2045952105 cites W2072853080 @default.
• W2045952105 cites W2078508576 @default.
• W2045952105 cites W2084950106 @default.
• W2045952105 cites W2087314630 @default.
• W2045952105 cites W2089759688 @default.
• W2045952105 cites W2090131332 @default.
• W2045952105 cites W2097651244 @default.
• W2045952105 cites W2099780173 @default.
• W2045952105 cites W2105963914 @default.
• W2045952105 cites W2112513166 @default.
• W2045952105 cites W2115843873 @default.
• W2045952105 cites W2121659651 @default.
• W2045952105 cites W2135025383 @default.
• W2045952105 cites W2139653511 @default.
• W2045952105 cites W2140800845 @default.
• W2045952105 cites W2141623970 @default.
• W2045952105 cites W2151603866 @default.
• W2045952105 cites W2152114427 @default.
• W2045952105 cites W2152261749 @default.
• W2045952105 cites W2154632774 @default.
• W2045952105 cites W2158666214 @default.
• W2045952105 cites W2162607594 @default.
• W2045952105 cites W2178513559 @default.
• W2045952105 cites W31182719 @default.
• W2045952105 doi "https://doi.org/10.1128/jvi.77.5.3020-3030.2003" @default.
• W2045952105 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/149732" @default.
• W2045952105 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12584327" @default.
• W2045952105 hasPublicationYear "2003" @default.
• W2045952105 type Work @default.
• W2045952105 sameAs 2045952105 @default.
• W2045952105 citedByCount "43" @default.
• W2045952105 countsByYear W20459521052012 @default.
• W2045952105 countsByYear W20459521052013 @default.
• W2045952105 countsByYear W20459521052014 @default.
• W2045952105 countsByYear W20459521052018 @default.
• W2045952105 countsByYear W20459521052020 @default.
• W2045952105 countsByYear W20459521052021 @default.
• W2045952105 countsByYear W20459521052023 @default.
• W2045952105 crossrefType "journal-article" @default.
• W2045952105 hasAuthorship W2045952105A5019287445 @default.
• W2045952105 hasAuthorship W2045952105A5058499082 @default.
• W2045952105 hasAuthorship W2045952105A5072940644 @default.
• W2045952105 hasAuthorship W2045952105A5090186785 @default.
• W2045952105 hasAuthorship W2045952105A5091604665 @default.
• W2045952105 hasBestOaLocation W20459521052 @default.
• W2045952105 hasConcept C104317684 @default.
• W2045952105 hasConcept C12464233 @default.
• W2045952105 hasConcept C138885662 @default.
• W2045952105 hasConcept C141231307 @default.
• W2045952105 hasConcept C153911025 @default.
• W2045952105 hasConcept C156719811 @default.
• W2045952105 hasConcept C159047783 @default.
• W2045952105 hasConcept C179926584 @default.
• W2045952105 hasConcept C22667442 @default.
• W2045952105 hasConcept C2776294106 @default.
• W2045952105 hasConcept C2779535198 @default.
• W2045952105 hasConcept C41258723 @default.
• W2045952105 hasConcept C41895202 @default.
• W2045952105 hasConcept C44667518 @default.
• W2045952105 hasConcept C54355233 @default.

• next