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- W2045952826 endingPage "493" @default.
- W2045952826 startingPage "493" @default.
- W2045952826 abstract "Background Bacterial skin infections are common in dogs and humans. Keratinocytes have phenotypic features of nonprofessional antigen‐presenting cells and express various cytokines. However, little is known about the effects of antibiotics on inflammatory markers in canine keratinocytes. Hypothesis/Objectives To investigate inflammatory markers in canine progenitor epidermal keratinocytes ( CPEK s) and to determine the effects of selected antibiotics on these markers. Methods The CPEK s were exposed for 2–24 h to three concentrations of amoxicillin, cefalexin, sulfadimethoxine, sulfamethoxazole (or its nitroso metabolite), amikacin or enrofloxacin. Enzyme‐linked immunosorbent assay ( ELISA ) and immunocytochemistry were used to detect major histocompatibility complex ( MHC ) II . CD 40 and CXCR 1 [interleukin ( IL )‐8 receptor] were detected using ELISA . Secreted cytokines/chemokines were quantified using a multiplex kit. Results No MHC II protein was detected. CD 40 protein was found at 24 h, with levels being significantly increased by enrofloxacin. The CPEK s secreted no detectable monocyte chemotactic protein‐1; undetectable to low (picogram per millilitre range) concentrations of IL ‐6, IL ‐7, IL ‐10, IL ‐15, tumour necrosis factor‐α, interferon‐γ and granulocyte–macrophage colony‐stimulating factor; and high (nanogram per millilitre range) concentrations of IL‐8. Levels of IL‐8 increased over 24 h following cell proliferation. They were significantly increased by enrofloxacin after 8 h, and by cefalexin, sulfadimethoxine, sulfamethoxazole, its nitroso metabolite and enrofloxacin after 24 h. The CPEK s expressed CXCR1. Conclusions and clinical importance Canine progenitor epidermal keratinocytes express various inflammatory proteins, with expression profiles being affected by certain antibiotics. This supports previous work showing keratinocytes to be mediators of inflammation and demonstrates the potential pro‐inflammatory effects of certain antibiotics in the skin." @default.
- W2045952826 created "2016-06-24" @default.
- W2045952826 creator A5022337039 @default.
- W2045952826 creator A5057909484 @default.
- W2045952826 creator A5075294890 @default.
- W2045952826 creator A5075878308 @default.
- W2045952826 date "2014-08-19" @default.
- W2045952826 modified "2023-10-16" @default.
- W2045952826 title "Canine progenitor epidermal keratinocytes express various inflammatory markers, including interleukin-8 and CD40, which are affected by certain antibiotics" @default.
- W2045952826 cites W1487149987 @default.
- W2045952826 cites W1487695181 @default.
- W2045952826 cites W1493735200 @default.
- W2045952826 cites W1508488702 @default.
- W2045952826 cites W1611935467 @default.
- W2045952826 cites W1622697170 @default.
- W2045952826 cites W1753762092 @default.
- W2045952826 cites W1836110191 @default.
- W2045952826 cites W1915043898 @default.
- W2045952826 cites W1963803921 @default.
- W2045952826 cites W1964615456 @default.
- W2045952826 cites W1964806620 @default.
- W2045952826 cites W1968046151 @default.
- W2045952826 cites W1969209002 @default.
- W2045952826 cites W1976021356 @default.
- W2045952826 cites W1976545569 @default.
- W2045952826 cites W1980199807 @default.
- W2045952826 cites W1983663861 @default.
- W2045952826 cites W1984235919 @default.
- W2045952826 cites W1987874963 @default.
- W2045952826 cites W1992322336 @default.
- W2045952826 cites W1993019119 @default.
- W2045952826 cites W1998439798 @default.
- W2045952826 cites W2004101061 @default.
- W2045952826 cites W2005067517 @default.
- W2045952826 cites W2010833545 @default.
- W2045952826 cites W2012279192 @default.
- W2045952826 cites W2013920320 @default.
- W2045952826 cites W2014048895 @default.
- W2045952826 cites W2016204658 @default.
- W2045952826 cites W2016316911 @default.
- W2045952826 cites W2016786200 @default.
- W2045952826 cites W2017251581 @default.
- W2045952826 cites W2019117353 @default.
- W2045952826 cites W2026207566 @default.
- W2045952826 cites W2027488940 @default.
- W2045952826 cites W2028765057 @default.
- W2045952826 cites W2028797852 @default.
- W2045952826 cites W2033860529 @default.
- W2045952826 cites W2036011337 @default.
- W2045952826 cites W2036766995 @default.
- W2045952826 cites W2037598877 @default.
- W2045952826 cites W2042317361 @default.
- W2045952826 cites W2043553473 @default.
- W2045952826 cites W2043773693 @default.
- W2045952826 cites W2047344004 @default.
- W2045952826 cites W2049136621 @default.
- W2045952826 cites W2049242209 @default.
- W2045952826 cites W2051577060 @default.
- W2045952826 cites W2052110525 @default.
- W2045952826 cites W2058195223 @default.
- W2045952826 cites W2059900785 @default.
- W2045952826 cites W2063638849 @default.
- W2045952826 cites W2064596960 @default.
- W2045952826 cites W2065289488 @default.
- W2045952826 cites W2065612614 @default.
- W2045952826 cites W2066405897 @default.
- W2045952826 cites W2068385730 @default.
- W2045952826 cites W2070050941 @default.
- W2045952826 cites W2071069256 @default.
- W2045952826 cites W2075158987 @default.
- W2045952826 cites W2076582065 @default.
- W2045952826 cites W2079357438 @default.
- W2045952826 cites W2080576360 @default.
- W2045952826 cites W2082530948 @default.
- W2045952826 cites W2085563465 @default.
- W2045952826 cites W2087747552 @default.
- W2045952826 cites W2088158890 @default.
- W2045952826 cites W2089081860 @default.
- W2045952826 cites W2089466202 @default.
- W2045952826 cites W2091464318 @default.
- W2045952826 cites W2095302068 @default.
- W2045952826 cites W2095774013 @default.
- W2045952826 cites W2098382778 @default.
- W2045952826 cites W2099843909 @default.
- W2045952826 cites W2100023667 @default.
- W2045952826 cites W2101553565 @default.
- W2045952826 cites W2101791639 @default.
- W2045952826 cites W2103593498 @default.
- W2045952826 cites W2104247216 @default.
- W2045952826 cites W2107566528 @default.
- W2045952826 cites W2110235919 @default.
- W2045952826 cites W2136492742 @default.
- W2045952826 cites W2140247836 @default.
- W2045952826 cites W2146829832 @default.
- W2045952826 cites W2148767345 @default.
- W2045952826 cites W2149085603 @default.
- W2045952826 cites W2150417288 @default.
- W2045952826 cites W2156086375 @default.