Matches in SemOpenAlex for { <https://semopenalex.org/work/W2045954702> ?p ?o ?g. }
- W2045954702 endingPage "e47482" @default.
- W2045954702 startingPage "e47482" @default.
- W2045954702 abstract "Renal interstitial fibrosis is characterized by increased extracellular matrix (ECM) synthesis. Epithelial-mesenchymal transition (EMT) in kidneys is driven by regulated expression of fibrogenic cytokines such as transforming growth factor-beta (TGF-β). Yam, or Dioscorea alata (DA) is an important herb in Chinese medicine widely used for the treatment of clinical diabetes mellitus. However, the fibrosis regulatory effect of DA is unclear. Thus, we examined TGF-β signaling mechanisms against EMT in rat fibroblast cells (NRK-49F). The characterization of DA water-extracts used various methods; after inducing cellular fibrosis in NRK-49F cells by treatment with β-hydroxybutyrate (β-HB) (10 mM), we used Western blotting to examine the protein expression in the TGF-β-related signal protein type I and type II TGF-β receptors, Smads2 and Smad3 (Smad2/3), pSmad2 and Smad3 (pSmad2/3), Smads4, Smads7, and EMT markers. These markers included E-cadherin, alpha-smooth muscle actin (α-SMA), and matrix metalloproteinase-2 (MMP-2). Bioactive TGF-β and fibronectin levels in the culture media were determined using ELISA. Expressions of fibronectin and Snail transcription factor, an EMT-regulatory transcription factor, were assessed by immunofluorescence staining. DA extract dose-dependently (50–200 µg/mL) suppressed β-HB-induced expression of fibronectin in NRK-49F cells concomitantly with the inhibition of Smad2/3, pSmad2/3, and Smad4. By contrast, Smad7 expression was significantly increased. DA extract caused a decrease in α-SMA (α-smooth muscle actin) and MMP-2 levels, and an increase in E-cadherin expression. We propose that DA extract might act as a novel fibrosis antagonist, which acts partly by down regulating the TGF-β/smad signaling pathway and modulating EMT expression." @default.
- W2045954702 created "2016-06-24" @default.
- W2045954702 creator A5000432258 @default.
- W2045954702 creator A5006125769 @default.
- W2045954702 creator A5009249253 @default.
- W2045954702 creator A5033571558 @default.
- W2045954702 creator A5034774683 @default.
- W2045954702 creator A5047673446 @default.
- W2045954702 creator A5048686813 @default.
- W2045954702 creator A5048947681 @default.
- W2045954702 creator A5055286127 @default.
- W2045954702 creator A5058564653 @default.
- W2045954702 creator A5085289129 @default.
- W2045954702 date "2012-11-08" @default.
- W2045954702 modified "2023-09-26" @default.
- W2045954702 title "Dioscorea alata Attenuates Renal Interstitial Cellular Fibrosis by Regulating Smad- and Epithelial-Mesenchymal Transition Signaling Pathways" @default.
- W2045954702 cites W1584414955 @default.
- W2045954702 cites W1964177773 @default.
- W2045954702 cites W1984760520 @default.
- W2045954702 cites W2001048683 @default.
- W2045954702 cites W2027693573 @default.
- W2045954702 cites W2083970331 @default.
- W2045954702 cites W2088876544 @default.
- W2045954702 cites W2091153586 @default.
- W2045954702 cites W2092344861 @default.
- W2045954702 cites W2106887481 @default.
- W2045954702 cites W2113456660 @default.
- W2045954702 cites W2128520582 @default.
- W2045954702 cites W2132803823 @default.
- W2045954702 cites W2133193835 @default.
- W2045954702 cites W2144891220 @default.
- W2045954702 cites W2148184356 @default.
- W2045954702 cites W2166890244 @default.
- W2045954702 cites W2316067769 @default.
- W2045954702 cites W2318993770 @default.
- W2045954702 cites W2333269488 @default.
- W2045954702 cites W4244774747 @default.
- W2045954702 doi "https://doi.org/10.1371/journal.pone.0047482" @default.
- W2045954702 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3493576" @default.
- W2045954702 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23144821" @default.
- W2045954702 hasPublicationYear "2012" @default.
- W2045954702 type Work @default.
- W2045954702 sameAs 2045954702 @default.
- W2045954702 citedByCount "22" @default.
- W2045954702 countsByYear W20459547022013 @default.
- W2045954702 countsByYear W20459547022014 @default.
- W2045954702 countsByYear W20459547022015 @default.
- W2045954702 countsByYear W20459547022016 @default.
- W2045954702 countsByYear W20459547022017 @default.
- W2045954702 countsByYear W20459547022019 @default.
- W2045954702 countsByYear W20459547022020 @default.
- W2045954702 countsByYear W20459547022022 @default.
- W2045954702 crossrefType "journal-article" @default.
- W2045954702 hasAuthorship W2045954702A5000432258 @default.
- W2045954702 hasAuthorship W2045954702A5006125769 @default.
- W2045954702 hasAuthorship W2045954702A5009249253 @default.
- W2045954702 hasAuthorship W2045954702A5033571558 @default.
- W2045954702 hasAuthorship W2045954702A5034774683 @default.
- W2045954702 hasAuthorship W2045954702A5047673446 @default.
- W2045954702 hasAuthorship W2045954702A5048686813 @default.
- W2045954702 hasAuthorship W2045954702A5048947681 @default.
- W2045954702 hasAuthorship W2045954702A5055286127 @default.
- W2045954702 hasAuthorship W2045954702A5058564653 @default.
- W2045954702 hasAuthorship W2045954702A5085289129 @default.
- W2045954702 hasBestOaLocation W20459547021 @default.
- W2045954702 hasConcept C104317684 @default.
- W2045954702 hasConcept C118131993 @default.
- W2045954702 hasConcept C126322002 @default.
- W2045954702 hasConcept C127561419 @default.
- W2045954702 hasConcept C189165786 @default.
- W2045954702 hasConcept C2778229498 @default.
- W2045954702 hasConcept C2780559512 @default.
- W2045954702 hasConcept C2781080636 @default.
- W2045954702 hasConcept C502942594 @default.
- W2045954702 hasConcept C55493867 @default.
- W2045954702 hasConcept C62478195 @default.
- W2045954702 hasConcept C71924100 @default.
- W2045954702 hasConcept C76419328 @default.
- W2045954702 hasConcept C86492073 @default.
- W2045954702 hasConcept C86803240 @default.
- W2045954702 hasConcept C95444343 @default.
- W2045954702 hasConceptScore W2045954702C104317684 @default.
- W2045954702 hasConceptScore W2045954702C118131993 @default.
- W2045954702 hasConceptScore W2045954702C126322002 @default.
- W2045954702 hasConceptScore W2045954702C127561419 @default.
- W2045954702 hasConceptScore W2045954702C189165786 @default.
- W2045954702 hasConceptScore W2045954702C2778229498 @default.
- W2045954702 hasConceptScore W2045954702C2780559512 @default.
- W2045954702 hasConceptScore W2045954702C2781080636 @default.
- W2045954702 hasConceptScore W2045954702C502942594 @default.
- W2045954702 hasConceptScore W2045954702C55493867 @default.
- W2045954702 hasConceptScore W2045954702C62478195 @default.
- W2045954702 hasConceptScore W2045954702C71924100 @default.
- W2045954702 hasConceptScore W2045954702C76419328 @default.
- W2045954702 hasConceptScore W2045954702C86492073 @default.
- W2045954702 hasConceptScore W2045954702C86803240 @default.
- W2045954702 hasConceptScore W2045954702C95444343 @default.
- W2045954702 hasIssue "11" @default.