Matches in SemOpenAlex for { <https://semopenalex.org/work/W2045958324> ?p ?o ?g. }
- W2045958324 endingPage "594" @default.
- W2045958324 startingPage "591" @default.
- W2045958324 abstract "Development of recombinant Chinese Hamster Ovary (CHO) cells producing therapeutic proteins requires analyzing the quality, such as sialic acid content, of proteins produced by many cell clones. In order to perform these analyses, high-throughput methods are required. Conventional methods for quantifying sialic acid, however, require protein purification, which is time consuming and cannot be used for high-throughput analysis. Here we used a high-throughput method (HTM) that we recently developed to analyze the intraclonal variability of 24 CHO cell subclones. The sialic acid content varied significantly from 1 to 70 mg sialic acid/g protein, and the concentration of total proteins secreted by the cells varied from 41 to 214 mg/L. In addition, the sialic acid content was negatively correlated with total protein concentration. This trend agrees with previous theoretical and experimental studies. Overall, the HTM can finish these analyses in 15 minutes, while conventional methods used in previous studies will require at least 24 days. Thus, the HTM can significantly accelerate the analyses of clonal and intraclonal variability in cell line development" @default.
- W2045958324 created "2016-06-24" @default.
- W2045958324 creator A5036630572 @default.
- W2045958324 creator A5056807501 @default.
- W2045958324 date "2011-11-07" @default.
- W2045958324 modified "2023-10-17" @default.
- W2045958324 title "High-throughput analysis of intraclonal variability of glycoprotein sialylation" @default.
- W2045958324 cites W1573656797 @default.
- W2045958324 cites W1798528947 @default.
- W2045958324 cites W1967681366 @default.
- W2045958324 cites W1971680669 @default.
- W2045958324 cites W1972148441 @default.
- W2045958324 cites W1982168520 @default.
- W2045958324 cites W1983692406 @default.
- W2045958324 cites W1985159109 @default.
- W2045958324 cites W1992708827 @default.
- W2045958324 cites W1996060786 @default.
- W2045958324 cites W2006994535 @default.
- W2045958324 cites W2014720377 @default.
- W2045958324 cites W2027515477 @default.
- W2045958324 cites W2035433348 @default.
- W2045958324 cites W2039083746 @default.
- W2045958324 cites W2042797223 @default.
- W2045958324 cites W2045486179 @default.
- W2045958324 cites W2049324939 @default.
- W2045958324 cites W2052231275 @default.
- W2045958324 cites W2053953032 @default.
- W2045958324 cites W2066499437 @default.
- W2045958324 cites W2068391557 @default.
- W2045958324 cites W2068623317 @default.
- W2045958324 cites W2069349248 @default.
- W2045958324 cites W2070890352 @default.
- W2045958324 cites W2086627779 @default.
- W2045958324 cites W2102649152 @default.
- W2045958324 cites W2122094998 @default.
- W2045958324 cites W2125007780 @default.
- W2045958324 cites W2139312302 @default.
- W2045958324 cites W2139404681 @default.
- W2045958324 cites W2154258517 @default.
- W2045958324 cites W2155218352 @default.
- W2045958324 cites W2157766920 @default.
- W2045958324 cites W2157858653 @default.
- W2045958324 cites W2160650963 @default.
- W2045958324 doi "https://doi.org/10.1002/btpr.738" @default.
- W2045958324 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22065488" @default.
- W2045958324 hasPublicationYear "2011" @default.
- W2045958324 type Work @default.
- W2045958324 sameAs 2045958324 @default.
- W2045958324 citedByCount "3" @default.
- W2045958324 countsByYear W20459583242012 @default.
- W2045958324 countsByYear W20459583242013 @default.
- W2045958324 countsByYear W20459583242015 @default.
- W2045958324 crossrefType "journal-article" @default.
- W2045958324 hasAuthorship W2045958324A5036630572 @default.
- W2045958324 hasAuthorship W2045958324A5056807501 @default.
- W2045958324 hasConcept C104317684 @default.
- W2045958324 hasConcept C108625454 @default.
- W2045958324 hasConcept C153911025 @default.
- W2045958324 hasConcept C175656101 @default.
- W2045958324 hasConcept C206212055 @default.
- W2045958324 hasConcept C2780115458 @default.
- W2045958324 hasConcept C40767141 @default.
- W2045958324 hasConcept C54355233 @default.
- W2045958324 hasConcept C55493867 @default.
- W2045958324 hasConcept C81885089 @default.
- W2045958324 hasConcept C86803240 @default.
- W2045958324 hasConcept C95444343 @default.
- W2045958324 hasConceptScore W2045958324C104317684 @default.
- W2045958324 hasConceptScore W2045958324C108625454 @default.
- W2045958324 hasConceptScore W2045958324C153911025 @default.
- W2045958324 hasConceptScore W2045958324C175656101 @default.
- W2045958324 hasConceptScore W2045958324C206212055 @default.
- W2045958324 hasConceptScore W2045958324C2780115458 @default.
- W2045958324 hasConceptScore W2045958324C40767141 @default.
- W2045958324 hasConceptScore W2045958324C54355233 @default.
- W2045958324 hasConceptScore W2045958324C55493867 @default.
- W2045958324 hasConceptScore W2045958324C81885089 @default.
- W2045958324 hasConceptScore W2045958324C86803240 @default.
- W2045958324 hasConceptScore W2045958324C95444343 @default.
- W2045958324 hasIssue "2" @default.
- W2045958324 hasLocation W20459583241 @default.
- W2045958324 hasLocation W20459583242 @default.
- W2045958324 hasOpenAccess W2045958324 @default.
- W2045958324 hasPrimaryLocation W20459583241 @default.
- W2045958324 hasRelatedWork W1998519759 @default.
- W2045958324 hasRelatedWork W2037741753 @default.
- W2045958324 hasRelatedWork W2067069447 @default.
- W2045958324 hasRelatedWork W2083274803 @default.
- W2045958324 hasRelatedWork W2367939407 @default.
- W2045958324 hasRelatedWork W2548757857 @default.
- W2045958324 hasRelatedWork W2771266500 @default.
- W2045958324 hasRelatedWork W2996675449 @default.
- W2045958324 hasRelatedWork W3022964734 @default.
- W2045958324 hasRelatedWork W4233442397 @default.
- W2045958324 hasVolume "28" @default.
- W2045958324 isParatext "false" @default.
- W2045958324 isRetracted "false" @default.
- W2045958324 magId "2045958324" @default.