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• W2045972663 endingPage "988" @default.
• W2045972663 startingPage "971" @default.
• W2045972663 abstract "A protein-protein Förster resonance energy transfer (FRET) system, employing probes at multiple positions, was designed to specifically monitor the dissociation of the H2A-H2B dimer from the nucleosome core particle (NCP). Tryptophan donors and Cys-AEDANS acceptors were chosen because, compared to previous NCP FRET fluorophores, they: (1) are smaller and less hydrophobic, which should minimize perturbations of histone and NCP structure; and (2) have an R0 of 20 A, which is much less than the dimensions of the NCP (approximately 50 A width and approximately 100 A diameter). Equilibrium protein unfolding titrations indicate that the donor and acceptor moieties have minimal effects on the stability of the H2A-H2B dimer and (H3-H4)2 tetramer. NCPs containing the various FRET pairs were reconstituted with the 601 DNA positioning element. Equilibrium NaCl-induced dissociation of the modified NCPs showed that the 601 sequence stabilized the NCP to dimer dissociation relative to weaker positioning sequences. This finding implies a significant role for the H2A-H2B dimers in determining the DNA sequence dependence of NCP stability. The free energy of dissociation determined from reversible and well-defined sigmoidal transitions revealed two distinct phases reflecting the dissociation of individual H2A-H2B dimers, confirming cooperativity as suggested previously; these data allow quantitative description of the cooperativity. The FRET system was then used to study the effects of the histone variant H2A.Z on NCP stability; previous studies have reported both destabilizing and stabilizing effects. H2A.Z FRET NCP dissociation transitions suggest a slight increase in stability but a significant increase in cooperativity of the dimer dissociations. Thus, the utility of this protein-protein FRET system to monitor the effects of histone variants on NCP dynamics has been demonstrated, and the system appears equally well-suited for dissection of the kinetic processes of dimer association and dissociation from the NCP." @default.
• W2045972663 created "2016-06-24" @default.
• W2045972663 creator A5010411578 @default.
• W2045972663 creator A5039282339 @default.
• W2045972663 creator A5076969060 @default.
• W2045972663 date "2007-08-01" @default.
• W2045972663 modified "2023-10-05" @default.
• W2045972663 title "Protein–Protein Förster Resonance Energy Transfer Analysis of Nucleosome Core Particles Containing H2A and H2A.Z" @default.
• W2045972663 cites W1485551308 @default.
• W2045972663 cites W1489564189 @default.
• W2045972663 cites W1569973595 @default.
• W2045972663 cites W1571482483 @default.
• W2045972663 cites W1575582671 @default.
• W2045972663 cites W160911776 @default.
• W2045972663 cites W166696829 @default.
• W2045972663 cites W1766348042 @default.
• W2045972663 cites W1796485970 @default.
• W2045972663 cites W1822988701 @default.
• W2045972663 cites W1876643910 @default.
• W2045972663 cites W1963611529 @default.
• W2045972663 cites W1965416025 @default.
• W2045972663 cites W1968817845 @default.
• W2045972663 cites W1970071803 @default.
• W2045972663 cites W1976179026 @default.
• W2045972663 cites W1977387171 @default.
• W2045972663 cites W1986046103 @default.
• W2045972663 cites W1986625994 @default.
• W2045972663 cites W1986915838 @default.
• W2045972663 cites W1987020026 @default.
• W2045972663 cites W1988997227 @default.
• W2045972663 cites W2000029728 @default.
• W2045972663 cites W2002057067 @default.
• W2045972663 cites W2003379794 @default.
• W2045972663 cites W2005986251 @default.
• W2045972663 cites W2008156174 @default.
• W2045972663 cites W2009702999 @default.
• W2045972663 cites W2009723835 @default.
• W2045972663 cites W2010048885 @default.
• W2045972663 cites W2010405881 @default.
• W2045972663 cites W2011847751 @default.
• W2045972663 cites W2015128418 @default.
• W2045972663 cites W2017076970 @default.
• W2045972663 cites W2017559669 @default.
• W2045972663 cites W2018145138 @default.
• W2045972663 cites W2020884463 @default.
• W2045972663 cites W2020979431 @default.
• W2045972663 cites W2021568742 @default.
• W2045972663 cites W2028270123 @default.
• W2045972663 cites W2030023744 @default.
• W2045972663 cites W2032969093 @default.
• W2045972663 cites W2034892963 @default.
• W2045972663 cites W2034961486 @default.
• W2045972663 cites W2039154165 @default.
• W2045972663 cites W2039564969 @default.
• W2045972663 cites W2052842503 @default.
• W2045972663 cites W2056422999 @default.
• W2045972663 cites W2061111901 @default.
• W2045972663 cites W2067939598 @default.
• W2045972663 cites W2073467330 @default.
• W2045972663 cites W2073552107 @default.
• W2045972663 cites W2083369623 @default.
• W2045972663 cites W2083762869 @default.
• W2045972663 cites W2088565678 @default.
• W2045972663 cites W2090674036 @default.
• W2045972663 cites W2091429260 @default.
• W2045972663 cites W2102123683 @default.
• W2045972663 cites W2102350205 @default.
• W2045972663 cites W2108608169 @default.
• W2045972663 cites W2113478190 @default.
• W2045972663 cites W2115369674 @default.
• W2045972663 cites W2116076986 @default.
• W2045972663 cites W2117747873 @default.
• W2045972663 cites W2122911345 @default.
• W2045972663 cites W2127205858 @default.
• W2045972663 cites W2128542570 @default.
• W2045972663 cites W2129314778 @default.
• W2045972663 cites W2132440338 @default.
• W2045972663 cites W2144792857 @default.
• W2045972663 cites W2145924925 @default.
• W2045972663 cites W2150402790 @default.
• W2045972663 cites W2151553287 @default.
• W2045972663 cites W2152406248 @default.
• W2045972663 cites W2461465024 @default.
• W2045972663 cites W3193208203 @default.
• W2045972663 cites W4247418383 @default.
• W2045972663 cites W995681685 @default.
• W2045972663 doi "https://doi.org/10.1016/j.jmb.2007.05.075" @default.
• W2045972663 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2031861" @default.
• W2045972663 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17597150" @default.
• W2045972663 hasPublicationYear "2007" @default.
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