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- W2045978620 abstract "Cardiac myocytes isolated from adult rats can be kept in culture with physiological calcium concentrations up to four days. Over this time cardiocytes preserve their normal ultrastructure. At intercalated disc area they round up and develop pseudopodia. With exogenous creatine energy rich metabolites are maintained at levels known from intact tissue. Isolated cells are mechanically at rest, their O2 consumption is comparable with values measured in resting hearts. With 50 μm 2,4-DNP they can be stimulated to values comparable with maximal O2 consumption of working hearts. Freshly isolated cells are either morphologically and metabolically intact and electromechanically coupled or rounded up and metabolically inactive. After 3h in culture a preparation of almost 100% intact cells can be obtained, since only intact cells attach to the dish." @default.
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- W2045978620 date "1982-07-01" @default.
- W2045978620 modified "2023-10-18" @default.
- W2045978620 title "Culturing of calcium stable adult cardiac myocytes" @default.
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- W2045978620 doi "https://doi.org/10.1016/0022-2828(82)90171-7" @default.
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