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- W2045978669 abstract "The anatomical distribution of specific [3H]kainate binding in goldfish brain was investigated by membrane binding and autoradiographical techniques. Saturation binding of the radioligand was determined in 8 anatomically defined regions and demonstrated a single class of high affinity sites with Kd values ranging from 290 to 650 nM. Kainate receptor densities, however, varied significantly. The cerebellum contained the highest concentration of binding sites (964 pmol/mg prot.), while the optic tectum had the lowest (96 pmol/mg prot.). Binding site distributions determined by autoradiographic studies demonstrated the same regional variation and allowed more specific localization of the binding sites. Within the cerebellum, the molecular layers of the corpus, valvula and lobus caudalis displayed a uniform and highly intense image while the granule cell layers (except for the medial granule cell mass of the lobus caudalis) did not. Other areas of intense binding were the posterior tubercle of the diencephalon, inferior lobes of the hypothalamus and layers 1 and 2 of the optic tectum (deep to the periventricular granule cells)." @default.
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- W2045978669 date "1990-09-01" @default.
- W2045978669 modified "2023-10-18" @default.
- W2045978669 title "[3H]Kainate localization in goldfish brain: receptor autoradiography and membrane binding" @default.
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- W2045978669 doi "https://doi.org/10.1016/0006-8993(90)91151-6" @default.
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