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• W2046003922 abstract "Photoporation is a rapidly expanding technique for the introduction of macromolecules into single cells. However, there remains no study into the true efficiency of this procedure. Here, we present a detailed analysis of transfection efficiency and cell viability for femtosecond optical transfection using a titanium sapphire laser at 800 nm. Photoporation of 4000 Chinese Hamster ovary cells was performed, representing the largest optical transfection study reported to date. We have investigated a range of laser fluences at the cell membrane and, at 1.2 microJ/cm(2), have found an average transfection efficiency of 50 +/- 10%. Contrary to recent literature, in which 100% efficiency is claimed, our measure of efficiency accounts for all irradiated cells, including those lost as a result of laser treatment, thereby providing a true biological measure of the technique." @default.
• W2046003922 created "2016-06-24" @default.
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• W2046003922 date "2006-01-01" @default.
• W2046003922 modified "2023-10-09" @default.
• W2046003922 title "Femtosecond optical transfection of cells:viability and efficiency" @default.
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• W2046003922 doi "https://doi.org/10.1364/oe.14.007125" @default.
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