FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2046004902> ?p ?o ?g. }

• W2046004902 endingPage "494" @default.
• W2046004902 startingPage "485" @default.
• W2046004902 abstract "GABA is an important inhibitory transmitter in the CNS. In the enteric nervous system, however, both excitatory and inhibitory actions have been reported. Here, we investigated the effects of GABA on the intracellular Ca2+ concentration of guinea-pig myenteric neurons (at 35°C) using Fura-2-AM. Neurons were identified by 75mM K+ depolarization (5 s), which evoked a transient intracellular Ca2+ concentration increase. GABA (10 s) induced a dose dependent (5nM–1μM) transient intracellular Ca2+ concentration rise in the majority of neurons (500nM GABA: 251±17nM, n=232/289). Interestingly, the response to 5μM GABA (n=18) lasted several minutes and did not fully recover. GABA response amplitudes were significantly (P<0.001) reduced by GABAA and GABAB receptor antagonists (10μM) bicuculline and phaclofen. The GABAA agonist isoguvacine (10μM) and GABAB agonist baclofen (10μM) induced similar responses as 50nM GABA, while the GABAC agonist cis-4-aminocrotonic acid (CACA) (10μM) only elicited small responses in a minority of neurons. Removal of extracellular Ca2+ abolished all responses while depletion of intracellular Ca2+ stores by thapsigargin (5μM) did not alter the responses to 500nM GABA (n=13), but reduction of Ca2+ influx through voltage-dependent Ca2+ channels did. The nicotinic antagonist hexamethonium (100μM) also reduced GABA responses by almost 70% suggesting that GABA stimulates cholinergic pathways, while the purinergic receptor blocker pyridoxal-phosphate-6-azophenyl-2′,4′-disulfonic acid (PPADS) and the 5-HT3 receptor blocker ondansetron only had minor effects. Conclusion: GABA elicits transient intracellular Ca2+ concentration responses in the majority of myenteric neurons through activation of GABAA and GABAB receptors and much of the response can be attributed to facilitation of ACh release. Thus GABA may act mainly as a modulator that sets the state of excitability of the enteric nerve network. A concentration of 5μM GABA, although frequently used in pharmacological experiments, seems to cause a detrimental response reminiscent of the neurotoxic effects glutamate has in the CNS." @default.
• W2046004902 created "2016-06-24" @default.
• W2046004902 creator A5010992910 @default.
• W2046004902 creator A5044812020 @default.
• W2046004902 creator A5064085748 @default.
• W2046004902 creator A5082202542 @default.
• W2046004902 date "2006-01-01" @default.
• W2046004902 modified "2023-09-23" @default.
• W2046004902 title "GABA-induced calcium signaling in cultured enteric neurons is reinforced by activation of cholinergic pathways" @default.
• W2046004902 cites W1520420711 @default.
• W2046004902 cites W1565700832 @default.
• W2046004902 cites W1677265049 @default.
• W2046004902 cites W18581828 @default.
• W2046004902 cites W1968806070 @default.
• W2046004902 cites W1969838876 @default.
• W2046004902 cites W1970189677 @default.
• W2046004902 cites W1974097137 @default.
• W2046004902 cites W1974684277 @default.
• W2046004902 cites W1975384712 @default.
• W2046004902 cites W1976514842 @default.
• W2046004902 cites W1978100753 @default.
• W2046004902 cites W1982344195 @default.
• W2046004902 cites W1985100400 @default.
• W2046004902 cites W1987314539 @default.
• W2046004902 cites W1987404669 @default.
• W2046004902 cites W1992220407 @default.
• W2046004902 cites W1994262885 @default.
• W2046004902 cites W1996954690 @default.
• W2046004902 cites W2002356340 @default.
• W2046004902 cites W2007522610 @default.
• W2046004902 cites W2008078824 @default.
• W2046004902 cites W2010449653 @default.
• W2046004902 cites W2011367927 @default.
• W2046004902 cites W2019132666 @default.
• W2046004902 cites W2026530081 @default.
• W2046004902 cites W2028558015 @default.
• W2046004902 cites W2029325710 @default.
• W2046004902 cites W2036605182 @default.
• W2046004902 cites W2045725221 @default.
• W2046004902 cites W2051918554 @default.
• W2046004902 cites W2053738062 @default.
• W2046004902 cites W2057018076 @default.
• W2046004902 cites W2057444949 @default.
• W2046004902 cites W2065719749 @default.
• W2046004902 cites W2072472560 @default.
• W2046004902 cites W2072974257 @default.
• W2046004902 cites W2079625703 @default.
• W2046004902 cites W2082101497 @default.
• W2046004902 cites W2085420542 @default.
• W2046004902 cites W2088840821 @default.
• W2046004902 cites W2096905627 @default.
• W2046004902 cites W2134766199 @default.
• W2046004902 cites W2153178009 @default.
• W2046004902 cites W2213569814 @default.
• W2046004902 cites W2334465950 @default.
• W2046004902 doi "https://doi.org/10.1016/j.neuroscience.2005.12.023" @default.
• W2046004902 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16446040" @default.
• W2046004902 hasPublicationYear "2006" @default.
• W2046004902 type Work @default.
• W2046004902 sameAs 2046004902 @default.
• W2046004902 citedByCount "15" @default.
• W2046004902 countsByYear W20460049022012 @default.
• W2046004902 countsByYear W20460049022013 @default.
• W2046004902 countsByYear W20460049022014 @default.
• W2046004902 countsByYear W20460049022015 @default.
• W2046004902 countsByYear W20460049022018 @default.
• W2046004902 countsByYear W20460049022022 @default.
• W2046004902 crossrefType "journal-article" @default.
• W2046004902 hasAuthorship W2046004902A5010992910 @default.
• W2046004902 hasAuthorship W2046004902A5044812020 @default.
• W2046004902 hasAuthorship W2046004902A5064085748 @default.
• W2046004902 hasAuthorship W2046004902A5082202542 @default.
• W2046004902 hasConcept C101027131 @default.
• W2046004902 hasConcept C126322002 @default.
• W2046004902 hasConcept C134018914 @default.
• W2046004902 hasConcept C168258287 @default.
• W2046004902 hasConcept C170493617 @default.
• W2046004902 hasConcept C17077164 @default.
• W2046004902 hasConcept C185592680 @default.
• W2046004902 hasConcept C22885893 @default.
• W2046004902 hasConcept C2775893369 @default.
• W2046004902 hasConcept C2775910092 @default.
• W2046004902 hasConcept C2778938600 @default.
• W2046004902 hasConcept C2780763569 @default.
• W2046004902 hasConcept C55493867 @default.
• W2046004902 hasConcept C71924100 @default.
• W2046004902 hasConcept C86803240 @default.
• W2046004902 hasConcept C98274493 @default.
• W2046004902 hasConceptScore W2046004902C101027131 @default.
• W2046004902 hasConceptScore W2046004902C126322002 @default.
• W2046004902 hasConceptScore W2046004902C134018914 @default.
• W2046004902 hasConceptScore W2046004902C168258287 @default.
• W2046004902 hasConceptScore W2046004902C170493617 @default.
• W2046004902 hasConceptScore W2046004902C17077164 @default.
• W2046004902 hasConceptScore W2046004902C185592680 @default.
• W2046004902 hasConceptScore W2046004902C22885893 @default.
• W2046004902 hasConceptScore W2046004902C2775893369 @default.
• W2046004902 hasConceptScore W2046004902C2775910092 @default.

• next