FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2046035394> ?p ?o ?g. }

• W2046035394 endingPage "121" @default.
• W2046035394 startingPage "117" @default.
• W2046035394 abstract "As a tool for the study of the capping-methylation process of yeast mRNA, we developed a procedure for the purification of the mRNA (guanine-7-)methyltransferase using the commercial cap analog guanosine(5′)triphospho(5′)guanosine as a substrate and radioactive S-adenosylmethionine (AdoMet) as the methyl group donor. The osmotic-sensitive yeast strain VY 1160 was used as the enzyme source. Little methyltransferase activity was detectable in a crude lysate obtained after osmotic shock. We showed that this was due to the presence of a low-molecular-weight inhibitor which could easily be eliminated by Sephadex G-25 gel filtration. The 10000 ×g supernatant from the crude lysate was submitted to DEAE-cellulose and DNA-agarose chromatography. The resulting preparation was enriched about 450-fold in specific activity. Under standard assay conditions, the incorporation rate remained constant for at least 6 h at 30°C. Transmethylation was not stimulated by KCl nor NaCl. Divalent cations were strong inhibitors. The partially purified enzyme was able to methylate undermethylated poly(A)-rich mRNA isolated from an AdoMet auxotrophic yeast strain briefly exposed to AdoMet-free medium." @default.
• W2046035394 created "2016-06-24" @default.
• W2046035394 creator A5009991935 @default.
• W2046035394 creator A5030702597 @default.
• W2046035394 creator A5044049055 @default.
• W2046035394 date "1983-07-01" @default.
• W2046035394 modified "2023-09-26" @default.
• W2046035394 title "Partial Purification and Characterization of mRNA (Guanine-7-) Methyltransferase from the Yeast Saccharomyces cerevisiae" @default.
• W2046035394 cites W108564182 @default.
• W2046035394 cites W1487743630 @default.
• W2046035394 cites W1503468971 @default.
• W2046035394 cites W1575815794 @default.
• W2046035394 cites W1980622219 @default.
• W2046035394 cites W2000154625 @default.
• W2046035394 cites W2046828533 @default.
• W2046035394 cites W2061270677 @default.
• W2046035394 cites W2063916020 @default.
• W2046035394 cites W2068598934 @default.
• W2046035394 cites W2100837269 @default.
• W2046035394 cites W2128635872 @default.
• W2046035394 cites W2503681065 @default.
• W2046035394 cites W41220400 @default.
• W2046035394 cites W4238099995 @default.
• W2046035394 doi "https://doi.org/10.1111/j.1432-1033.1983.tb07539.x" @default.
• W2046035394 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6345158" @default.
• W2046035394 hasPublicationYear "1983" @default.
• W2046035394 type Work @default.
• W2046035394 sameAs 2046035394 @default.
• W2046035394 citedByCount "10" @default.
• W2046035394 crossrefType "journal-article" @default.
• W2046035394 hasAuthorship W2046035394A5009991935 @default.
• W2046035394 hasAuthorship W2046035394A5030702597 @default.
• W2046035394 hasAuthorship W2046035394A5044049055 @default.
• W2046035394 hasBestOaLocation W20460353941 @default.
• W2046035394 hasConcept C104317684 @default.
• W2046035394 hasConcept C153911025 @default.
• W2046035394 hasConcept C163994747 @default.
• W2046035394 hasConcept C178790620 @default.
• W2046035394 hasConcept C181199279 @default.
• W2046035394 hasConcept C185592680 @default.
• W2046035394 hasConcept C193363816 @default.
• W2046035394 hasConcept C2777576037 @default.
• W2046035394 hasConcept C2777995097 @default.
• W2046035394 hasConcept C2778301229 @default.
• W2046035394 hasConcept C2779222958 @default.
• W2046035394 hasConcept C2781259782 @default.
• W2046035394 hasConcept C28774466 @default.
• W2046035394 hasConcept C33288867 @default.
• W2046035394 hasConcept C43617362 @default.
• W2046035394 hasConcept C512185932 @default.
• W2046035394 hasConcept C552990157 @default.
• W2046035394 hasConcept C55493867 @default.
• W2046035394 hasConcept C57409179 @default.
• W2046035394 hasConcept C67705224 @default.
• W2046035394 hasConcept C74884574 @default.
• W2046035394 hasConcept C86803240 @default.
• W2046035394 hasConcept C91965660 @default.
• W2046035394 hasConceptScore W2046035394C104317684 @default.
• W2046035394 hasConceptScore W2046035394C153911025 @default.
• W2046035394 hasConceptScore W2046035394C163994747 @default.
• W2046035394 hasConceptScore W2046035394C178790620 @default.
• W2046035394 hasConceptScore W2046035394C181199279 @default.
• W2046035394 hasConceptScore W2046035394C185592680 @default.
• W2046035394 hasConceptScore W2046035394C193363816 @default.
• W2046035394 hasConceptScore W2046035394C2777576037 @default.
• W2046035394 hasConceptScore W2046035394C2777995097 @default.
• W2046035394 hasConceptScore W2046035394C2778301229 @default.
• W2046035394 hasConceptScore W2046035394C2779222958 @default.
• W2046035394 hasConceptScore W2046035394C2781259782 @default.
• W2046035394 hasConceptScore W2046035394C28774466 @default.
• W2046035394 hasConceptScore W2046035394C33288867 @default.
• W2046035394 hasConceptScore W2046035394C43617362 @default.
• W2046035394 hasConceptScore W2046035394C512185932 @default.
• W2046035394 hasConceptScore W2046035394C552990157 @default.
• W2046035394 hasConceptScore W2046035394C55493867 @default.
• W2046035394 hasConceptScore W2046035394C57409179 @default.
• W2046035394 hasConceptScore W2046035394C67705224 @default.
• W2046035394 hasConceptScore W2046035394C74884574 @default.
• W2046035394 hasConceptScore W2046035394C86803240 @default.
• W2046035394 hasConceptScore W2046035394C91965660 @default.
• W2046035394 hasIssue "1" @default.
• W2046035394 hasLocation W20460353941 @default.
• W2046035394 hasLocation W20460353942 @default.
• W2046035394 hasOpenAccess W2046035394 @default.
• W2046035394 hasPrimaryLocation W20460353941 @default.
• W2046035394 hasRelatedWork W1562281848 @default.
• W2046035394 hasRelatedWork W1596097154 @default.
• W2046035394 hasRelatedWork W1793371687 @default.
• W2046035394 hasRelatedWork W1985480577 @default.
• W2046035394 hasRelatedWork W2023386970 @default.
• W2046035394 hasRelatedWork W2046035394 @default.
• W2046035394 hasRelatedWork W2076854625 @default.
• W2046035394 hasRelatedWork W2389579098 @default.
• W2046035394 hasRelatedWork W2399538604 @default.
• W2046035394 hasRelatedWork W2414763538 @default.
• W2046035394 hasVolume "134" @default.
• W2046035394 isParatext "false" @default.
• W2046035394 isRetracted "false" @default.

• next