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- W204607515 abstract "MUTIHA PANJAITAN. Construction of Mutant Pseudomonas sp. CRB 17 to Increase Indole Acetic Acid Production by Transposon Mutagenesis. Supervised by ARIS TRI WAHYUDI and NISA RACHMANIA MUBARIK. Many bacteria living in the rhizosphere contribute as a plant growth promoter called as plant growth promoting rhizobacteria. One of them is Pseudomonas sp.. One hundred isolates categorized as Pseudomonas sp. was succesfully isolated from soybean rhizosphere soil. The isolate Pseudomonas sp. CRB 17 produce highest amount of indole acetic acid (IAA) at 16.02 ppm. To increase IAA production, transposon mutagenesis had been done using Escherichia coli S17-1 (λ pir), carrying miniTn5Km1 (pUTmini-Tn5Km1) as the donor and Pseudomonas sp. CRB 17 as the recipient strain. The frequency of conjugation obtained was approximately 3.1 x 10 cell per recipient for 24 hours incubation time of conjugation. Forty nine mutants have been analyzed for their IAA production. These mutants produced a varying amount of IAA, ranging from mutants which have decreasing IAA production down to 55.31 %, mutants with moderate IAA production, and the remaining with increasing IAA production up to 77.52 %. From the results of this research can be concluded that transposon mutagenesis can be used to increase IAA production of Pseudomonas sp. CRB 17. KONSTRUKSI MUTAN Pseudomonas sp. CRB 17 UNTUK MENINGKATKAN PRODUKSI ASAM INDOL ASETAT MELALUI MUTAGENESIS DENGAN TRANSPOSON" @default.
- W204607515 created "2016-06-24" @default.
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- W204607515 date "2007-01-01" @default.
- W204607515 modified "2023-09-27" @default.
- W204607515 title "Konstruksi Mutan Pseudomonas sp. CRB17 untuk Meningkatkan Produksi Asam Indol Asetat melalui Mutagenesis dengan Transposon" @default.
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- W204607515 hasPublicationYear "2007" @default.
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