FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2046084246> ?p ?o ?g. }

• W2046084246 endingPage "29" @default.
• W2046084246 startingPage "29" @default.
• W2046084246 abstract "Evaluation of antidiphtheria toxin nanobodies Ghada H ShakerDepartment of Microbiology and Immunology, Faculty of Pharmacy, King Saud University, Riyadh, Kingdom of Saudi ArabiaAbstract: Nanobodies are the smallest fragments of naturally occurring single-domain antibodies that have evolved to be fully functional in the absence of a light chain. Conventional antibodies are glycoproteins comprising two heavy and two light chains. Surprisingly, all members of the Camelidae family possess a fraction of antibodies devoid of both light chains and the first constant domain. These types of antibodies are known as heavy-chain antibody (HcAb) nanobodies. There are three subclasses of IgG in dromedaries, namely IgG1, IgG2, and IgG3 of which IgG2 and IgG3 are of the HcAb type. These heavy chain antibodies constitute approximately 50% of the IgG in llama serum and as much as 75% of the IgG in camel serum. In the present work, the different IgG subclasses from an immunized camel (Camelus dromedarius) with divalent diphtheria-tetanus vaccine were purified using their different affinity for protein A and protein G and their absorbance measured at 280 nm. Purity control and characterization by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis of IgG subclasses was done under reducing conditions. Protein bands were visualized after staining with Coomassie Blue, showing two bands at 50 kDa and 30 kDa for IgG1, while IgG2 and IgG3 produced only one band at 46 kDa and 43 kDa, respectively. An enzyme-linked immunosorbent assay test using diphtheria toxin and purified IgG subclasses from the immunized camel were performed to evaluate their efficiency. Compared with conventional IgG1, heavy chain antibodies (nanobodies) were shown to be more efficient in binding to diphtheria toxin antigen. This study revealed the possibility of using IgG2 and IgG3 nanobodies as an effective antitoxin for the treatment of diphtheria in humans.Keywords: camel, heavy chain antibody, HcAb, nanobodies, immunoglobulin, IgG subclasses, diphtheria toxin" @default.
• W2046084246 created "2016-06-24" @default.
• W2046084246 creator A5051467292 @default.
• W2046084246 date "2010-06-01" @default.
• W2046084246 modified "2023-09-25" @default.
• W2046084246 title "Evaluation of antidiphtheria toxin nanobodies" @default.
• W2046084246 cites W1649136725 @default.
• W2046084246 cites W1961017490 @default.
• W2046084246 cites W1984969969 @default.
• W2046084246 cites W1989672802 @default.
• W2046084246 cites W2001144575 @default.
• W2046084246 cites W2011376078 @default.
• W2046084246 cites W2020368956 @default.
• W2046084246 cites W2027285268 @default.
• W2046084246 cites W2029386192 @default.
• W2046084246 cites W2033079214 @default.
• W2046084246 cites W2034516179 @default.
• W2046084246 cites W2037594142 @default.
• W2046084246 cites W2039226199 @default.
• W2046084246 cites W2055273433 @default.
• W2046084246 cites W2057278101 @default.
• W2046084246 cites W2062653170 @default.
• W2046084246 cites W2066485051 @default.
• W2046084246 cites W2073940609 @default.
• W2046084246 cites W2075024118 @default.
• W2046084246 cites W2075673875 @default.
• W2046084246 cites W2076718235 @default.
• W2046084246 cites W2079971222 @default.
• W2046084246 cites W2087240786 @default.
• W2046084246 cites W2095553811 @default.
• W2046084246 cites W2099092534 @default.
• W2046084246 cites W2100151882 @default.
• W2046084246 cites W2100837269 @default.
• W2046084246 cites W2104341428 @default.
• W2046084246 cites W2161709523 @default.
• W2046084246 cites W2347134342 @default.
• W2046084246 doi "https://doi.org/10.2147/nsa.s9591" @default.
• W2046084246 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3781745" @default.
• W2046084246 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24198468" @default.
• W2046084246 hasPublicationYear "2010" @default.
• W2046084246 type Work @default.
• W2046084246 sameAs 2046084246 @default.
• W2046084246 citedByCount "9" @default.
• W2046084246 countsByYear W20460842462015 @default.
• W2046084246 countsByYear W20460842462017 @default.
• W2046084246 countsByYear W20460842462018 @default.
• W2046084246 countsByYear W20460842462019 @default.
• W2046084246 countsByYear W20460842462020 @default.
• W2046084246 countsByYear W20460842462022 @default.
• W2046084246 countsByYear W20460842462023 @default.
• W2046084246 crossrefType "journal-article" @default.
• W2046084246 hasAuthorship W2046084246A5051467292 @default.
• W2046084246 hasBestOaLocation W20460842461 @default.
• W2046084246 hasConcept C153911025 @default.
• W2046084246 hasConcept C159654299 @default.
• W2046084246 hasConcept C185592680 @default.
• W2046084246 hasConcept C203014093 @default.
• W2046084246 hasConcept C2777367657 @default.
• W2046084246 hasConcept C2778913249 @default.
• W2046084246 hasConcept C2780761556 @default.
• W2046084246 hasConcept C33057221 @default.
• W2046084246 hasConcept C36394416 @default.
• W2046084246 hasConcept C54355233 @default.
• W2046084246 hasConcept C55493867 @default.
• W2046084246 hasConcept C74864618 @default.
• W2046084246 hasConcept C86803240 @default.
• W2046084246 hasConceptScore W2046084246C153911025 @default.
• W2046084246 hasConceptScore W2046084246C159654299 @default.
• W2046084246 hasConceptScore W2046084246C185592680 @default.
• W2046084246 hasConceptScore W2046084246C203014093 @default.
• W2046084246 hasConceptScore W2046084246C2777367657 @default.
• W2046084246 hasConceptScore W2046084246C2778913249 @default.
• W2046084246 hasConceptScore W2046084246C2780761556 @default.
• W2046084246 hasConceptScore W2046084246C33057221 @default.
• W2046084246 hasConceptScore W2046084246C36394416 @default.
• W2046084246 hasConceptScore W2046084246C54355233 @default.
• W2046084246 hasConceptScore W2046084246C55493867 @default.
• W2046084246 hasConceptScore W2046084246C74864618 @default.
• W2046084246 hasConceptScore W2046084246C86803240 @default.
• W2046084246 hasLocation W20460842461 @default.
• W2046084246 hasLocation W20460842462 @default.
• W2046084246 hasLocation W20460842463 @default.
• W2046084246 hasLocation W20460842464 @default.
• W2046084246 hasOpenAccess W2046084246 @default.
• W2046084246 hasPrimaryLocation W20460842461 @default.
• W2046084246 hasRelatedWork W136714500 @default.
• W2046084246 hasRelatedWork W1912318859 @default.
• W2046084246 hasRelatedWork W1977733520 @default.
• W2046084246 hasRelatedWork W1984249145 @default.
• W2046084246 hasRelatedWork W2037307292 @default.
• W2046084246 hasRelatedWork W2062722834 @default.
• W2046084246 hasRelatedWork W2092172148 @default.
• W2046084246 hasRelatedWork W2886840708 @default.
• W2046084246 hasRelatedWork W2886877484 @default.
• W2046084246 hasRelatedWork W3217412447 @default.
• W2046084246 isParatext "false" @default.
• W2046084246 isRetracted "false" @default.
• W2046084246 magId "2046084246" @default.

• next