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• W2046093285 abstract "The objective of the present study was to produce a generic monoclonal antibody for phenothiazine drugs. Phenothiazine was derivatized with sodium chloroacetate to synthesize a generic hapten that was then coupled with bovine serum albumin to prepare the immunogen by use of n-hydroxysuccinimide active ester method. Hybridomas were obtained by fusing mouse myeloma cells SP2/0 with the splenocyte cells from the mice immunized with the immunogen. Then, a competitive indirect enzyme-linked immunosorbent assay was used to determine the recognization capacity of the obtained monoclonal antibody for phenothiazine drugs. Results showed that the monoclonal antibody of phenothiazine simultaneously recognized five phenothiazine drugs (chlorpromazine, promethazine, acepromazin, perphenazine, and fluphenazine). The cross-reactivities were in the range of 42%–87% and the limits of detection were in the range of 0.2–0.7 ng/mL. Therefore, the obtained monoclonal antibody in this study was suitable for the development of an immunoassay to multi-detect the five phenothiazine drugs." @default.
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• W2046093285 date "2014-03-12" @default.
• W2046093285 modified "2023-10-01" @default.
• W2046093285 title "Synthesis of novel hapten and production of the generic monoclonal antibody for phenothiazine drugs" @default.
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• W2046093285 doi "https://doi.org/10.1080/03601234.2014.882159" @default.
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