FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2046119079> ?p ?o ?g. }

• W2046119079 endingPage "269" @default.
• W2046119079 startingPage "259" @default.
• W2046119079 abstract "20beta-hydroxysteroid dehydrogenase (20beta-HSD) synthesizes 17alpha,20beta-dihdroxy-4-pregnen-3-one, the steroid required for resumption of prophase-I arrested oocytes in teleosts. Though 20beta-HSD cDNAs have been cloned from few fish species, its role in final oocyte maturation (FOM) is still questionable. To study the role of 20beta-HSD in FOM more explicitly, we cloned and characterized 20beta-HSD from ovary of air-breathing catfish, Clarias gariepinus. Interestingly, Escherichia coli expressed recombinant proteins, both full-length and an N-terminal truncated proteins catalyzed the reduction of steroids and xenobiotics, however there was significant difference between them. Semi-quantitative RT-PCR and Western blots demonstrated the presence of 20beta-HSD transcript and protein in various tissues with relatively higher level in gonads, gill, kidney and brain. A positive correlation of 20beta-HSD expression was observed in different phases of ovarian cycles. Immunocytochemical/immunofluoroscence analysis with specific antibody identified presence of 20beta-HSD in follicular layer of ovary. Real-time RT-PCR and Western blotting showed an induction of 20beta-HSD expression during human chorionic gonadotropin (hCG)-induced oocyte maturation, in vitro and in vivo. Concomitantly, a rise in 20beta-HSD enzyme activity was also noticed. Specific inhibitors of carbonyl reductase inhibited not only recombinant protein catalytic activity but also hCG-induced oocyte maturation in a dose-dependent manner as evidenced by blocking of germinal vesicle break down. These results together provide new evidences for the involvement of 20beta-HSD in the FOM/meiotic maturation." @default.
• W2046119079 created "2016-06-24" @default.
• W2046119079 creator A5027095634 @default.
• W2046119079 creator A5058365668 @default.
• W2046119079 date "2009-09-01" @default.
• W2046119079 modified "2023-09-23" @default.
• W2046119079 title "New evidences for the involvement of 20β-hydroxysteroid dehydrogenase in final oocyte maturation of air-breathing catfish" @default.
• W2046119079 cites W1519712150 @default.
• W2046119079 cites W1680105951 @default.
• W2046119079 cites W1972999545 @default.
• W2046119079 cites W1981909865 @default.
• W2046119079 cites W1983389305 @default.
• W2046119079 cites W1988992341 @default.
• W2046119079 cites W2008053811 @default.
• W2046119079 cites W2014234155 @default.
• W2046119079 cites W2022728827 @default.
• W2046119079 cites W2023606507 @default.
• W2046119079 cites W2030270383 @default.
• W2046119079 cites W2047076612 @default.
• W2046119079 cites W2047894301 @default.
• W2046119079 cites W2050431727 @default.
• W2046119079 cites W2051620814 @default.
• W2046119079 cites W2056550338 @default.
• W2046119079 cites W2061789423 @default.
• W2046119079 cites W2064265703 @default.
• W2046119079 cites W2076857075 @default.
• W2046119079 cites W2078279857 @default.
• W2046119079 cites W2081676841 @default.
• W2046119079 cites W2085457011 @default.
• W2046119079 cites W2085643546 @default.
• W2046119079 cites W2111500030 @default.
• W2046119079 cites W2121638331 @default.
• W2046119079 cites W2126754491 @default.
• W2046119079 cites W2135319921 @default.
• W2046119079 cites W2138585688 @default.
• W2046119079 cites W2149410074 @default.
• W2046119079 cites W2158635396 @default.
• W2046119079 cites W4250778245 @default.
• W2046119079 cites W4293247451 @default.
• W2046119079 cites W528192684 @default.
• W2046119079 doi "https://doi.org/10.1016/j.ygcen.2009.04.022" @default.
• W2046119079 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19401204" @default.
• W2046119079 hasPublicationYear "2009" @default.
• W2046119079 type Work @default.
• W2046119079 sameAs 2046119079 @default.
• W2046119079 citedByCount "27" @default.
• W2046119079 countsByYear W20461190792012 @default.
• W2046119079 countsByYear W20461190792013 @default.
• W2046119079 countsByYear W20461190792014 @default.
• W2046119079 countsByYear W20461190792015 @default.
• W2046119079 countsByYear W20461190792016 @default.
• W2046119079 countsByYear W20461190792017 @default.
• W2046119079 countsByYear W20461190792020 @default.
• W2046119079 countsByYear W20461190792021 @default.
• W2046119079 countsByYear W20461190792022 @default.
• W2046119079 countsByYear W20461190792023 @default.
• W2046119079 crossrefType "journal-article" @default.
• W2046119079 hasAuthorship W2046119079A5027095634 @default.
• W2046119079 hasAuthorship W2046119079A5058365668 @default.
• W2046119079 hasConcept C153911025 @default.
• W2046119079 hasConcept C196843134 @default.
• W2046119079 hasConcept C2776690073 @default.
• W2046119079 hasConcept C2778189562 @default.
• W2046119079 hasConcept C2909208804 @default.
• W2046119079 hasConcept C2994119143 @default.
• W2046119079 hasConcept C505870484 @default.
• W2046119079 hasConcept C56366318 @default.
• W2046119079 hasConcept C86803240 @default.
• W2046119079 hasConcept C95444343 @default.
• W2046119079 hasConceptScore W2046119079C153911025 @default.
• W2046119079 hasConceptScore W2046119079C196843134 @default.
• W2046119079 hasConceptScore W2046119079C2776690073 @default.
• W2046119079 hasConceptScore W2046119079C2778189562 @default.
• W2046119079 hasConceptScore W2046119079C2909208804 @default.
• W2046119079 hasConceptScore W2046119079C2994119143 @default.
• W2046119079 hasConceptScore W2046119079C505870484 @default.
• W2046119079 hasConceptScore W2046119079C56366318 @default.
• W2046119079 hasConceptScore W2046119079C86803240 @default.
• W2046119079 hasConceptScore W2046119079C95444343 @default.
• W2046119079 hasIssue "3" @default.
• W2046119079 hasLocation W20461190791 @default.
• W2046119079 hasLocation W20461190792 @default.
• W2046119079 hasOpenAccess W2046119079 @default.
• W2046119079 hasPrimaryLocation W20461190791 @default.
• W2046119079 hasRelatedWork W1607994503 @default.
• W2046119079 hasRelatedWork W1993582742 @default.
• W2046119079 hasRelatedWork W2022326994 @default.
• W2046119079 hasRelatedWork W2085490784 @default.
• W2046119079 hasRelatedWork W2087602002 @default.
• W2046119079 hasRelatedWork W2144905883 @default.
• W2046119079 hasRelatedWork W2328243806 @default.
• W2046119079 hasRelatedWork W2388861505 @default.
• W2046119079 hasRelatedWork W2411058307 @default.
• W2046119079 hasRelatedWork W4224123241 @default.
• W2046119079 hasVolume "163" @default.
• W2046119079 isParatext "false" @default.
• W2046119079 isRetracted "false" @default.
• W2046119079 magId "2046119079" @default.

• next