FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2046127532> ?p ?o ?g. }

• W2046127532 endingPage "6346" @default.
• W2046127532 startingPage "6338" @default.
• W2046127532 abstract "Methods to fine-tune the rate of a fast conformational electron transfer (ET) gate involving a His−heme alkaline conformer of iso-1-cytochrome c (iso-1-Cytc) and to adjust the pH accessibility of a slow ET gate involving a Lys−heme alkaline conformer are described. Fine-tuning the fast ET gate employs a strategy of making surface mutations in a substructure unfolded in the alkaline conformer. To make the slow ET gate accessible at neutral pH, the strategy involves mutations at buried sequence positions which are expected to more strongly perturb the stability of native versus alkaline iso-1-Cytc. To fine-tune the rate of the fast His 73−heme ET gate, we mutate the surface-exposed Lys 79 to Ala (A79H73 variant). This mutation also simplifies ET gating by removing Lys 79, which can serve as a ligand in the alkaline conformer of iso-1-Cytc. To adjust the pH accessibility of the slow Lys 73−heme ET gate, we convert the buried side chain Asn 52 to Gly and also mutate Lys 79 to Ala to simplify ET gating (A79G52 variant). ET kinetics is studied as a function of pH using hexaammineruthenium(II) chloride (a6Ru2+) to reduce the variants. Both variants show fast direct ET reactions dependent on [a6Ru2+] and slower gated ET reactions that are independent of [a6Ru2+]. The observed gated ET rates correlate well with rates for the alkaline-to-native state conformational change measured independently. Together with the previously reported H73 variant (Baddam, S.; Bowler, B. E. J. Am. Chem. Soc. 2005, 127, 9702−9703), the A79H73 variant allows His 73−heme-mediated ET gating to be fine-tuned from 75 to 200 ms. The slower Lys 73−heme (15−20 s time scale) ET gate for the A79G52 variant is now accessible over the pH range 6−8." @default.
• W2046127532 created "2016-06-24" @default.
• W2046127532 creator A5023826967 @default.
• W2046127532 creator A5074549149 @default.
• W2046127532 date "2006-08-01" @default.
• W2046127532 modified "2023-09-25" @default.
• W2046127532 title "Tuning the Rate and pH Accessibility of a Conformational Electron Transfer Gate" @default.
• W2046127532 cites W1486510394 @default.
• W2046127532 cites W1497306863 @default.
• W2046127532 cites W152087508 @default.
• W2046127532 cites W1555570350 @default.
• W2046127532 cites W1556387976 @default.
• W2046127532 cites W1572420301 @default.
• W2046127532 cites W1840994082 @default.
• W2046127532 cites W1965532585 @default.
• W2046127532 cites W1971915692 @default.
• W2046127532 cites W1973664438 @default.
• W2046127532 cites W1980120138 @default.
• W2046127532 cites W1984055082 @default.
• W2046127532 cites W1991859850 @default.
• W2046127532 cites W1994088455 @default.
• W2046127532 cites W1994571394 @default.
• W2046127532 cites W1996796347 @default.
• W2046127532 cites W1997787755 @default.
• W2046127532 cites W2009142811 @default.
• W2046127532 cites W2016709299 @default.
• W2046127532 cites W2018783426 @default.
• W2046127532 cites W2025001414 @default.
• W2046127532 cites W2031779266 @default.
• W2046127532 cites W2048829910 @default.
• W2046127532 cites W2049436067 @default.
• W2046127532 cites W2053508860 @default.
• W2046127532 cites W2053613163 @default.
• W2046127532 cites W2054702380 @default.
• W2046127532 cites W2055563200 @default.
• W2046127532 cites W2058626036 @default.
• W2046127532 cites W2059736644 @default.
• W2046127532 cites W2062468561 @default.
• W2046127532 cites W2067130595 @default.
• W2046127532 cites W2072110215 @default.
• W2046127532 cites W2081082999 @default.
• W2046127532 cites W2081291394 @default.
• W2046127532 cites W2081736815 @default.
• W2046127532 cites W2085070970 @default.
• W2046127532 cites W2091387166 @default.
• W2046127532 cites W2109683405 @default.
• W2046127532 cites W2134248388 @default.
• W2046127532 cites W2170485699 @default.
• W2046127532 cites W2949217956 @default.
• W2046127532 cites W2950219173 @default.
• W2046127532 cites W2952436916 @default.
• W2046127532 cites W2952953177 @default.
• W2046127532 cites W658839705 @default.
• W2046127532 doi "https://doi.org/10.1021/ic0603712" @default.
• W2046127532 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16878944" @default.
• W2046127532 hasPublicationYear "2006" @default.
• W2046127532 type Work @default.
• W2046127532 sameAs 2046127532 @default.
• W2046127532 citedByCount "16" @default.
• W2046127532 countsByYear W20461275322012 @default.
• W2046127532 countsByYear W20461275322013 @default.
• W2046127532 countsByYear W20461275322014 @default.
• W2046127532 countsByYear W20461275322015 @default.
• W2046127532 countsByYear W20461275322016 @default.
• W2046127532 countsByYear W20461275322018 @default.
• W2046127532 crossrefType "journal-article" @default.
• W2046127532 hasAuthorship W2046127532A5023826967 @default.
• W2046127532 hasAuthorship W2046127532A5074549149 @default.
• W2046127532 hasConcept C121332964 @default.
• W2046127532 hasConcept C123669783 @default.
• W2046127532 hasConcept C12554922 @default.
• W2046127532 hasConcept C148898269 @default.
• W2046127532 hasConcept C178790620 @default.
• W2046127532 hasConcept C181199279 @default.
• W2046127532 hasConcept C185592680 @default.
• W2046127532 hasConcept C18705241 @default.
• W2046127532 hasConcept C194544171 @default.
• W2046127532 hasConcept C2776217839 @default.
• W2046127532 hasConcept C28859421 @default.
• W2046127532 hasConcept C29311851 @default.
• W2046127532 hasConcept C32123652 @default.
• W2046127532 hasConcept C32909587 @default.
• W2046127532 hasConcept C55493867 @default.
• W2046127532 hasConcept C62520636 @default.
• W2046127532 hasConcept C71240020 @default.
• W2046127532 hasConcept C75473681 @default.
• W2046127532 hasConcept C8010536 @default.
• W2046127532 hasConcept C86803240 @default.
• W2046127532 hasConceptScore W2046127532C121332964 @default.
• W2046127532 hasConceptScore W2046127532C123669783 @default.
• W2046127532 hasConceptScore W2046127532C12554922 @default.
• W2046127532 hasConceptScore W2046127532C148898269 @default.
• W2046127532 hasConceptScore W2046127532C178790620 @default.
• W2046127532 hasConceptScore W2046127532C181199279 @default.
• W2046127532 hasConceptScore W2046127532C185592680 @default.
• W2046127532 hasConceptScore W2046127532C18705241 @default.
• W2046127532 hasConceptScore W2046127532C194544171 @default.
• W2046127532 hasConceptScore W2046127532C2776217839 @default.

• next