Matches in SemOpenAlex for { <https://semopenalex.org/work/W2046127556> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W2046127556 endingPage "S134" @default.
- W2046127556 startingPage "S134" @default.
- W2046127556 abstract "OBJECTIVE: The sperm nucleus contains diverse populations of RNA that are potentially transmitted to the oocyte at the time of fertilization. The functional role of these transcripts is uncertain, and could include contribution to embryogenesis and/or transcriptional gene silencing. This study investigated sperm RNA profiles in correlation with subsequent blastocyst development. DESIGN: Retrospective study. MATERIALS AND METHODS: Infertile couples (n=16) undergoing IVF using donor oocytes (female factor standardized), donated normozoospermic samples with consent. Cycles were divided into 2 groups relative to blastocyst quality: Group A (Good) = ≥25% of D5 blastocysts ≥Grade 3BB, and Group B (Poor) = <15% of D5 blastocysts ≥Grade 3BB. Total RNA was isolated from about 1 million sperm (ICSI preparation, Group A=8 and Group B=8) and reverse transcribed for quantitative real-time PCR. RESULTS: No significant differences were observed for paternal age, sperm parameters, fertilization rates, cleavage stage embryo grading or number of D5 blastocysts transferred between Groups A and B. Gene expression analysis of 17 transcripts involved in spermatogenesis and/or development were examined by quantitative real-time PCR relative to the internal housekeeping gene, PPIA. Three genes, AKAP4, CLU and HSBP, exhibited significantly decreased expression in Group B sperm samples compared with Group A (P<0.05). Both AKAP4 and CLU participate in biological processes related to development. Following D5 blastocyst transfer, implantation rate with fetal cardiac activity indicated a trend towards greater competence of Group A blastocysts (A=66.7% v. B=43.8%, ns). CONCLUSION: Differential sperm RNA profiles from donor oocyte IVF cycles reflected subsequent D5 blastocyst quality. In particular, genes related to development showed a decrease in expression in association with poor blastocyst development and competence. Further studies are required to determine if these sperm transcripts indeed play a functional role during embryogenesis." @default.
- W2046127556 created "2016-06-24" @default.
- W2046127556 creator A5007700345 @default.
- W2046127556 creator A5014315368 @default.
- W2046127556 creator A5024836733 @default.
- W2046127556 creator A5037237135 @default.
- W2046127556 creator A5064030969 @default.
- W2046127556 creator A5072168750 @default.
- W2046127556 date "2010-09-01" @default.
- W2046127556 modified "2023-09-25" @default.
- W2046127556 title "Differential sperm RNA profiles are associated with subsequent blastocyst development" @default.
- W2046127556 doi "https://doi.org/10.1016/j.fertnstert.2010.07.539" @default.
- W2046127556 hasPublicationYear "2010" @default.
- W2046127556 type Work @default.
- W2046127556 sameAs 2046127556 @default.
- W2046127556 citedByCount "0" @default.
- W2046127556 crossrefType "journal-article" @default.
- W2046127556 hasAuthorship W2046127556A5007700345 @default.
- W2046127556 hasAuthorship W2046127556A5014315368 @default.
- W2046127556 hasAuthorship W2046127556A5024836733 @default.
- W2046127556 hasAuthorship W2046127556A5037237135 @default.
- W2046127556 hasAuthorship W2046127556A5064030969 @default.
- W2046127556 hasAuthorship W2046127556A5072168750 @default.
- W2046127556 hasConcept C104317684 @default.
- W2046127556 hasConcept C150194340 @default.
- W2046127556 hasConcept C16685009 @default.
- W2046127556 hasConcept C196843134 @default.
- W2046127556 hasConcept C2776690073 @default.
- W2046127556 hasConcept C2778177303 @default.
- W2046127556 hasConcept C2781087480 @default.
- W2046127556 hasConcept C28748434 @default.
- W2046127556 hasConcept C54355233 @default.
- W2046127556 hasConcept C71924100 @default.
- W2046127556 hasConcept C86803240 @default.
- W2046127556 hasConcept C87073359 @default.
- W2046127556 hasConcept C88972607 @default.
- W2046127556 hasConceptScore W2046127556C104317684 @default.
- W2046127556 hasConceptScore W2046127556C150194340 @default.
- W2046127556 hasConceptScore W2046127556C16685009 @default.
- W2046127556 hasConceptScore W2046127556C196843134 @default.
- W2046127556 hasConceptScore W2046127556C2776690073 @default.
- W2046127556 hasConceptScore W2046127556C2778177303 @default.
- W2046127556 hasConceptScore W2046127556C2781087480 @default.
- W2046127556 hasConceptScore W2046127556C28748434 @default.
- W2046127556 hasConceptScore W2046127556C54355233 @default.
- W2046127556 hasConceptScore W2046127556C71924100 @default.
- W2046127556 hasConceptScore W2046127556C86803240 @default.
- W2046127556 hasConceptScore W2046127556C87073359 @default.
- W2046127556 hasConceptScore W2046127556C88972607 @default.
- W2046127556 hasIssue "4" @default.
- W2046127556 hasLocation W20461275561 @default.
- W2046127556 hasOpenAccess W2046127556 @default.
- W2046127556 hasPrimaryLocation W20461275561 @default.
- W2046127556 hasRelatedWork W1972453963 @default.
- W2046127556 hasRelatedWork W2033082795 @default.
- W2046127556 hasRelatedWork W2046745697 @default.
- W2046127556 hasRelatedWork W2113458525 @default.
- W2046127556 hasRelatedWork W2166971475 @default.
- W2046127556 hasRelatedWork W2349296746 @default.
- W2046127556 hasRelatedWork W2378094771 @default.
- W2046127556 hasRelatedWork W2389182588 @default.
- W2046127556 hasRelatedWork W2793393990 @default.
- W2046127556 hasRelatedWork W4223472953 @default.
- W2046127556 hasVolume "94" @default.
- W2046127556 isParatext "false" @default.
- W2046127556 isRetracted "false" @default.
- W2046127556 magId "2046127556" @default.
- W2046127556 workType "article" @default.