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• W2046163105 abstract "It remains a challenge to establish a straightforward genetic approach for controlling the probability of gene activation or knockout at a desired level. Here, we developed a method termed STARS: stochastic gene activation with genetically regulated sparseness. The stochastic expression was achieved by two cross-linked, mutually-exclusive Cre-mediated recombinations. The stochastic level was further controlled by regulating Cre/lox reaction kinetics through varying the intrachromosomal distance between the lox sites mediating one of the recombinations. In mammalian cell lines stably transfected with a single copy of different STARS transgenes, the activation/knockout of reporter genes was specifically controlled to occur in from 5% to 50% of the cell population. STARS can potentially provide a convenient way for genetic labeling as well as gene expression/knockout in a population of cells with a desired sparseness level." @default.
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• W2046163105 date "2009-01-15" @default.
• W2046163105 modified "2023-09-26" @default.
• W2046163105 title "A Genetic Strategy for Stochastic Gene Activation with Regulated Sparseness (STARS)" @default.
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• W2046163105 doi "https://doi.org/10.1371/journal.pone.0004200" @default.
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