FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2046163248> ?p ?o ?g. }

• W2046163248 endingPage "323" @default.
• W2046163248 startingPage "316" @default.
• W2046163248 abstract "The RTEM-1 thiol β-lactamase (Sigal, I. S., Harwood, B. G., Arentzen, R., Proc. Natl. Acad. Sci. USA. 79:7157–7160, 1982) is inactivated by thiol-selective reagents such as iodoacetamide, methyl methanethiosulfonate, and 4,4′-dipyridyldisulfide, which modify the active site thiol group. The pH-rate profiles of these inactivation reactions show that there are two nucleophilic forms of the enzyme, EH2 and EH, both of which, by analogy with the situation with cysteine proteinases, probably contain the active site nucleophile in the thiolate form. The pKa of the active site thiol is therefore shown by the data to be below 4.0. This low pKa is thought to reflect the presence of adjacent functionality which stabilizes the thiolate anion. The low nucleophilicity of the thiolate in both EH2 and EH, with respect to that of cysteine proteinases and model compounds, suggests that the thiolate of the thiol β-lactamase is stabilized by two hydrogen-bond donors. One of these, of pKa greater than 9.0, is suggested to be the conserved and essential Lys-73 ammonium group, of pKa around 6.7, is less clear, but may be the conserved Glu-166 carboxylic acid. β-Lactamase activity is associated with the EH2 form, and thus the β-lactamase active site is proposed to contain one basic or nucleophilic group (the thiolate in the thiol β-lactamase) and two acidic (hydrogen-bond donor) groups (one of which is likely to be the above-mentioned lysine ammonium group)." @default.
• W2046163248 created "2016-06-24" @default.
• W2046163248 creator A5067548741 @default.
• W2046163248 creator A5082781225 @default.
• W2046163248 date "1989-01-01" @default.
• W2046163248 modified "2023-10-14" @default.
• W2046163248 title "Chemical modification of the RTEM-1 thiol β-lactamase by thiol-selective reagents: Evidence for activation of the primary nucleophile of the β-lactamase active site by adjacent functional groups" @default.
• W2046163248 cites W124983324 @default.
• W2046163248 cites W1543749024 @default.
• W2046163248 cites W1862088956 @default.
• W2046163248 cites W1887614213 @default.
• W2046163248 cites W1922438834 @default.
• W2046163248 cites W1954141158 @default.
• W2046163248 cites W1978777049 @default.
• W2046163248 cites W1980629578 @default.
• W2046163248 cites W1998312296 @default.
• W2046163248 cites W2006370393 @default.
• W2046163248 cites W2014853483 @default.
• W2046163248 cites W2021996491 @default.
• W2046163248 cites W2026130809 @default.
• W2046163248 cites W2036474910 @default.
• W2046163248 cites W2036857388 @default.
• W2046163248 cites W2059215022 @default.
• W2046163248 cites W2069580699 @default.
• W2046163248 cites W2070527414 @default.
• W2046163248 cites W2079461414 @default.
• W2046163248 cites W2084514212 @default.
• W2046163248 cites W2095541771 @default.
• W2046163248 cites W2112636022 @default.
• W2046163248 cites W2119594150 @default.
• W2046163248 cites W2143874510 @default.
• W2046163248 cites W2189392965 @default.
• W2046163248 cites W2229311549 @default.
• W2046163248 cites W2232664651 @default.
• W2046163248 cites W2250613068 @default.
• W2046163248 cites W2279429215 @default.
• W2046163248 cites W2320037322 @default.
• W2046163248 cites W4211227459 @default.
• W2046163248 doi "https://doi.org/10.1002/prot.340060314" @default.
• W2046163248 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2695930" @default.
• W2046163248 hasPublicationYear "1989" @default.
• W2046163248 type Work @default.
• W2046163248 sameAs 2046163248 @default.
• W2046163248 citedByCount "13" @default.
• W2046163248 countsByYear W20461632482013 @default.
• W2046163248 countsByYear W20461632482020 @default.
• W2046163248 crossrefType "journal-article" @default.
• W2046163248 hasAuthorship W2046163248A5067548741 @default.
• W2046163248 hasAuthorship W2046163248A5082781225 @default.
• W2046163248 hasConcept C112887158 @default.
• W2046163248 hasConcept C155647269 @default.
• W2046163248 hasConcept C160964918 @default.
• W2046163248 hasConcept C161790260 @default.
• W2046163248 hasConcept C178790620 @default.
• W2046163248 hasConcept C178907741 @default.
• W2046163248 hasConcept C181199279 @default.
• W2046163248 hasConcept C185592680 @default.
• W2046163248 hasConcept C2776515853 @default.
• W2046163248 hasConcept C2779201268 @default.
• W2046163248 hasConcept C2781324293 @default.
• W2046163248 hasConcept C32909587 @default.
• W2046163248 hasConcept C40875361 @default.
• W2046163248 hasConcept C41183919 @default.
• W2046163248 hasConcept C71240020 @default.
• W2046163248 hasConceptScore W2046163248C112887158 @default.
• W2046163248 hasConceptScore W2046163248C155647269 @default.
• W2046163248 hasConceptScore W2046163248C160964918 @default.
• W2046163248 hasConceptScore W2046163248C161790260 @default.
• W2046163248 hasConceptScore W2046163248C178790620 @default.
• W2046163248 hasConceptScore W2046163248C178907741 @default.
• W2046163248 hasConceptScore W2046163248C181199279 @default.
• W2046163248 hasConceptScore W2046163248C185592680 @default.
• W2046163248 hasConceptScore W2046163248C2776515853 @default.
• W2046163248 hasConceptScore W2046163248C2779201268 @default.
• W2046163248 hasConceptScore W2046163248C2781324293 @default.
• W2046163248 hasConceptScore W2046163248C32909587 @default.
• W2046163248 hasConceptScore W2046163248C40875361 @default.
• W2046163248 hasConceptScore W2046163248C41183919 @default.
• W2046163248 hasConceptScore W2046163248C71240020 @default.
• W2046163248 hasIssue "3" @default.
• W2046163248 hasLocation W20461632481 @default.
• W2046163248 hasLocation W20461632482 @default.
• W2046163248 hasOpenAccess W2046163248 @default.
• W2046163248 hasPrimaryLocation W20461632481 @default.
• W2046163248 hasRelatedWork W1607200279 @default.
• W2046163248 hasRelatedWork W1823861229 @default.
• W2046163248 hasRelatedWork W1829095039 @default.
• W2046163248 hasRelatedWork W1998294214 @default.
• W2046163248 hasRelatedWork W2035970110 @default.
• W2046163248 hasRelatedWork W2046163248 @default.
• W2046163248 hasRelatedWork W2066451762 @default.
• W2046163248 hasRelatedWork W2080068879 @default.
• W2046163248 hasRelatedWork W2232664651 @default.
• W2046163248 hasRelatedWork W67226878 @default.
• W2046163248 hasVolume "6" @default.
• W2046163248 isParatext "false" @default.
• W2046163248 isRetracted "false" @default.
• W2046163248 magId "2046163248" @default.

• next