FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2046165061> ?p ?o ?g. }

• W2046165061 endingPage "126" @default.
• W2046165061 startingPage "118" @default.
• W2046165061 abstract "To facilitate the use of large-insert bacterial clones for functional analysis, we have constructed new bacterial artificial chromosome vectors, pPAC4 and pBACe4. These vectors contain two genetic elements that enable stable maintenance of the clones in mammalian cells: (1) The Epstein–Barr virus replicon, oriP, is included to ensure stable episomal propagation of the large insert clones upon transfection into mammalian cells. (2) The blasticidin deaminase gene is placed in a eukaryotic expression cassette to enable selection for the desired mammalian clones by using the nucleoside antibiotic blasticidin. Sequences important to select for loxP-specific genome targeting in mammalian chromosomes are also present. In addition, we demonstrate that the attTn7 sequence present on the vectors permits specific addition of selected features to the library clones. Unique sites have also been included in the vector to enable linearization of the large-insert clones, e.g., for optical mapping studies. The pPAC4 vector has been used to generate libraries from the human, mouse, and rat genomes. We believe that clones from these libraries would serve as an important reagent in functional experiments, including the identification or validation of candidate disease genes, by transferring a particular clone containing the relevant wildtype gene into mutant cells or transgenic or knock-out animals." @default.
• W2046165061 created "2016-06-24" @default.
• W2046165061 creator A5004413418 @default.
• W2046165061 creator A5023203908 @default.
• W2046165061 creator A5024248420 @default.
• W2046165061 creator A5033523495 @default.
• W2046165061 creator A5048678488 @default.
• W2046165061 creator A5049498078 @default.
• W2046165061 creator A5053961735 @default.
• W2046165061 creator A5059316091 @default.
• W2046165061 creator A5066231280 @default.
• W2046165061 creator A5091250120 @default.
• W2046165061 date "2000-09-01" @default.
• W2046165061 modified "2023-10-16" @default.
• W2046165061 title "Modular Bacterial Artificial Chromosome Vectors for Transfer of Large Inserts into Mammalian Cells" @default.
• W2046165061 cites W1508946785 @default.
• W2046165061 cites W1594478849 @default.
• W2046165061 cites W1675216 @default.
• W2046165061 cites W1827441151 @default.
• W2046165061 cites W1978836807 @default.
• W2046165061 cites W1994578639 @default.
• W2046165061 cites W1996749102 @default.
• W2046165061 cites W2004198466 @default.
• W2046165061 cites W2012288527 @default.
• W2046165061 cites W2015221134 @default.
• W2046165061 cites W2015327873 @default.
• W2046165061 cites W2020024953 @default.
• W2046165061 cites W2021608281 @default.
• W2046165061 cites W2033122034 @default.
• W2046165061 cites W2049650255 @default.
• W2046165061 cites W2050558334 @default.
• W2046165061 cites W2051818544 @default.
• W2046165061 cites W2052840428 @default.
• W2046165061 cites W2054933900 @default.
• W2046165061 cites W2059391361 @default.
• W2046165061 cites W2064788529 @default.
• W2046165061 cites W2065587065 @default.
• W2046165061 cites W2069546130 @default.
• W2046165061 cites W2069903573 @default.
• W2046165061 cites W2072295407 @default.
• W2046165061 cites W2077003408 @default.
• W2046165061 cites W2080118179 @default.
• W2046165061 cites W2081756307 @default.
• W2046165061 cites W2085453035 @default.
• W2046165061 cites W2104060691 @default.
• W2046165061 cites W2104854307 @default.
• W2046165061 cites W2114940149 @default.
• W2046165061 cites W2156617601 @default.
• W2046165061 cites W4238475667 @default.
• W2046165061 doi "https://doi.org/10.1006/geno.2000.6286" @default.
• W2046165061 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10964509" @default.
• W2046165061 hasPublicationYear "2000" @default.
• W2046165061 type Work @default.
• W2046165061 sameAs 2046165061 @default.
• W2046165061 citedByCount "39" @default.
• W2046165061 countsByYear W20461650612012 @default.
• W2046165061 countsByYear W20461650612013 @default.
• W2046165061 countsByYear W20461650612014 @default.
• W2046165061 crossrefType "journal-article" @default.
• W2046165061 hasAuthorship W2046165061A5004413418 @default.
• W2046165061 hasAuthorship W2046165061A5023203908 @default.
• W2046165061 hasAuthorship W2046165061A5024248420 @default.
• W2046165061 hasAuthorship W2046165061A5033523495 @default.
• W2046165061 hasAuthorship W2046165061A5048678488 @default.
• W2046165061 hasAuthorship W2046165061A5049498078 @default.
• W2046165061 hasAuthorship W2046165061A5053961735 @default.
• W2046165061 hasAuthorship W2046165061A5059316091 @default.
• W2046165061 hasAuthorship W2046165061A5066231280 @default.
• W2046165061 hasAuthorship W2046165061A5091250120 @default.
• W2046165061 hasConcept C104317684 @default.
• W2046165061 hasConcept C127413603 @default.
• W2046165061 hasConcept C141231307 @default.
• W2046165061 hasConcept C145640855 @default.
• W2046165061 hasConcept C182901222 @default.
• W2046165061 hasConcept C22744801 @default.
• W2046165061 hasConcept C2776441376 @default.
• W2046165061 hasConcept C2780098074 @default.
• W2046165061 hasConcept C40767141 @default.
• W2046165061 hasConcept C42411736 @default.
• W2046165061 hasConcept C54355233 @default.
• W2046165061 hasConcept C547475151 @default.
• W2046165061 hasConcept C58337764 @default.
• W2046165061 hasConcept C70721500 @default.
• W2046165061 hasConcept C73445445 @default.
• W2046165061 hasConcept C78519656 @default.
• W2046165061 hasConcept C81089528 @default.
• W2046165061 hasConcept C86803240 @default.
• W2046165061 hasConcept C92087593 @default.
• W2046165061 hasConceptScore W2046165061C104317684 @default.
• W2046165061 hasConceptScore W2046165061C127413603 @default.
• W2046165061 hasConceptScore W2046165061C141231307 @default.
• W2046165061 hasConceptScore W2046165061C145640855 @default.
• W2046165061 hasConceptScore W2046165061C182901222 @default.
• W2046165061 hasConceptScore W2046165061C22744801 @default.
• W2046165061 hasConceptScore W2046165061C2776441376 @default.
• W2046165061 hasConceptScore W2046165061C2780098074 @default.
• W2046165061 hasConceptScore W2046165061C40767141 @default.
• W2046165061 hasConceptScore W2046165061C42411736 @default.

• next