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- W2046167275 abstract "The cortical actomyosin layer below the membrane of dividing cells and the microtubules of the mitotic spindle are coupled through internal feedback mechanisms. Identifying the factors that control molecular motor distribution is essential for understanding cell division. The equatorial contractile ring forms by myosin assembly in the middle and disassembly in the flanking regions. To better understand the mechanical and biochemical mechanisms of contractile ring assembly, we imaged HeLa cells expressing MRLC-GFP by confocal microscopy at high spatial and temporal resolution. MRLC-GFP also marks spindle poles; this allowed us to study the correlation between mitotic spindle movements and cortical myosin distribution. We confirmed that the onset of myosin assembly in the contractile ring occurs in anaphase. We observed that the mitotic spindle exhibited oscillations upon entry into anaphase, similar to oscillations observed during the asymmetric division of C. elegans embryos by other groups. We used particle tracking software to follow spindle position and JFilament to track cortical myosin intensity distribution during these oscillations. We further exerted controlled and localized forces while following the cellular response using fluorescence imaging. Anti-integrin coated micro-beads were attached to HeLa cells expressing MRLC-GFP. We applied external forces to a bead by an optical tweezers and imaged the dynamics of myosin and mitotic spindle during cell division. Experimental results showed that the spindle appeared to move to an asymmetric post perturbation position. This observation is consistent with the expected coupling between force-dependent cortical flow and spindle position and will be useful in more detailed studies of the response of cytoskeleton to external or internal forces." @default.
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- W2046167275 date "2011-02-01" @default.
- W2046167275 modified "2023-09-28" @default.
- W2046167275 title "Myosin Cortical Assembly and Spindle Oscillations During Hela Cell Division" @default.
- W2046167275 doi "https://doi.org/10.1016/j.bpj.2010.12.2632" @default.
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