Matches in SemOpenAlex for { <https://semopenalex.org/work/W2046179624> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W2046179624 abstract "Abstract Three control mechanisms have been demonstrated in Bacillus megaterium which regulate the level of Δ5-desaturating enzyme, and hence the rate of unsaturated fatty acid biosynthesis, in response to temperature changes in the growth or incubation medium. One control process mediated by temperature is that of desaturase induction. A culture growing at 35° does not synthesize unsaturated fatty acids. When the culture is transferred to 20°, however, the synthesis of desaturase begins and continues at an accelerating rate for at least 1 hour. The induction process is blocked by chloramphenicol and the evidence suggests that both the desaturase synthesizing system and the desaturase itself are absent in cultures growing at 35° and must be induced at 20° by a process requiring protein synthesis. A second control process responsive to temperature is the irreversible inactivation of the desaturating enzyme. The inactivation process, in vivo, follows strict first order kinetics at all temperatures and the enzyme half-life is determined solely by the incubation temperature. The rate of inactivation of the desaturase is extremely sensitive to slight changes in temperature. At temperatures near 20° a decrease of less than 2° in the temperature of the incubation medium results in a more than 2-fold increase in the half-life of the desaturating enzyme. Temperature-mediiated irreversible inactivation is probably the most important mechanism for regulating the steady state level of desaturase during the exponential phase of cell growth at temperatures near 20°. A third process, the decay of the desaturase synthesizing system, is observed when a culture that has undergone induction at 20° is transferred back to 34°. Not only is the irreversible first order inactivation of the desaturating enzyme accelerated after transfer, but the desaturase-synthesizing system itself immediately begins to decay at a rate which follows zero order kinetics. The kinetics of decay as well as several other characteristics of the process are consistent with (but do not conclusively demonstrate) a mechanism involving the temperature-triggered synthesis of a repressor for the desaturase synthesis system. There is also some evidence for a rapid, temperature-reversible denaturation of desaturase at 34° which is superimposed on the slower, irreversible first order inactivation of the desaturating enzyme. Finally, equations are derived which enable one to calculate, as a function of time, the individual contribution of each of these temperature-dependent processes in regulating the level of desaturating enzyme at a given temperature in cultures of B. megaterium." @default.
- W2046179624 created "2016-06-24" @default.
- W2046179624 creator A5003397600 @default.
- W2046179624 date "1972-10-31" @default.
- W2046179624 modified "2023-09-27" @default.
- W2046179624 title "BIOSYNTHESIS OF UNSATURATED FATTY ACIDS BY BACILLI. IV. TEMPERATURE- MEDIATED CONTROL MECHANISMS." @default.
- W2046179624 cites W1518654272 @default.
- W2046179624 cites W1893289977 @default.
- W2046179624 cites W1899255695 @default.
- W2046179624 cites W1992258492 @default.
- W2046179624 cites W2008872573 @default.
- W2046179624 cites W2024611108 @default.
- W2046179624 cites W2033590997 @default.
- W2046179624 cites W2041079513 @default.
- W2046179624 cites W2077147114 @default.
- W2046179624 doi "https://doi.org/10.2172/4738479" @default.
- W2046179624 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4624119" @default.
- W2046179624 hasPublicationYear "1972" @default.
- W2046179624 type Work @default.
- W2046179624 sameAs 2046179624 @default.
- W2046179624 citedByCount "8" @default.
- W2046179624 crossrefType "report" @default.
- W2046179624 hasAuthorship W2046179624A5003397600 @default.
- W2046179624 hasBestOaLocation W20461796243 @default.
- W2046179624 hasConcept C104317684 @default.
- W2046179624 hasConcept C185592680 @default.
- W2046179624 hasConcept C2776870249 @default.
- W2046179624 hasConcept C31903555 @default.
- W2046179624 hasConcept C523546767 @default.
- W2046179624 hasConcept C54355233 @default.
- W2046179624 hasConcept C553450214 @default.
- W2046179624 hasConcept C55493867 @default.
- W2046179624 hasConcept C86803240 @default.
- W2046179624 hasConcept C89423630 @default.
- W2046179624 hasConceptScore W2046179624C104317684 @default.
- W2046179624 hasConceptScore W2046179624C185592680 @default.
- W2046179624 hasConceptScore W2046179624C2776870249 @default.
- W2046179624 hasConceptScore W2046179624C31903555 @default.
- W2046179624 hasConceptScore W2046179624C523546767 @default.
- W2046179624 hasConceptScore W2046179624C54355233 @default.
- W2046179624 hasConceptScore W2046179624C553450214 @default.
- W2046179624 hasConceptScore W2046179624C55493867 @default.
- W2046179624 hasConceptScore W2046179624C86803240 @default.
- W2046179624 hasConceptScore W2046179624C89423630 @default.
- W2046179624 hasLocation W20461796241 @default.
- W2046179624 hasLocation W20461796242 @default.
- W2046179624 hasLocation W20461796243 @default.
- W2046179624 hasOpenAccess W2046179624 @default.
- W2046179624 hasPrimaryLocation W20461796241 @default.
- W2046179624 hasRelatedWork W2027286229 @default.
- W2046179624 hasRelatedWork W2037061166 @default.
- W2046179624 hasRelatedWork W2067503442 @default.
- W2046179624 hasRelatedWork W2074089773 @default.
- W2046179624 hasRelatedWork W2132814393 @default.
- W2046179624 hasRelatedWork W2364408457 @default.
- W2046179624 hasRelatedWork W2368409219 @default.
- W2046179624 hasRelatedWork W2441940490 @default.
- W2046179624 hasRelatedWork W2444161153 @default.
- W2046179624 hasRelatedWork W2607069924 @default.
- W2046179624 isParatext "false" @default.
- W2046179624 isRetracted "false" @default.
- W2046179624 magId "2046179624" @default.
- W2046179624 workType "report" @default.